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Plasmid#228983PurposeFor bacterial expression of anti-GST nanobody GST-1, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-1
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-3
Plasmid#228985PurposeFor bacterial expression of anti-GST nanobody GST-3, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-3
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaTdT-44
Plasmid#228981PurposeFor bacterial expression of anti-tdTomato nanobody LaTdT-44, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertLaTdT-44
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaTdT-45
Plasmid#228982PurposeFor bacterial expression of anti-tdTomato nanobody LaTdT-45, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertLaTdT-45
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-8
Plasmid#228990PurposeFor bacterial expression of anti-GST nanobody GST-8, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-8
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-5
Plasmid#228987PurposeFor bacterial expression of anti-GST nanobody GST-5, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-5
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-6
Plasmid#228988PurposeFor bacterial expression of anti-GST nanobody GST-6, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-6
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCEFL-HAx2-TEADiv2
Plasmid#228873PurposeExpression of optimized TEAD inhibitor (TEADiv2): 2xHA-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signalDepositorInsertTEADiv2
UseTags2xHAExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaTdT-39
Plasmid#228980PurposeFor bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertLaTdT-39
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCEFL-EGFP-TEADiv2
Plasmid#228872PurposeExpression of optimized TEAD inhibitor (TEADiv2): GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signalDepositorInsertTEADiv2
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
EYFP-FKBP-DN-N-WASP
Plasmid#210301Purposecontrol for targeted actin nucleationDepositorInsertEYFP-FKBP-DN-N-WASP
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
EYFP-FKBP-N-WASP
Plasmid#210302Purposetargeted actin nucleationDepositorInsertEYFP-FKBP-N-WASP
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-3xGFP11-mem-3xControlgRNA
Plasmid#224583PurposeUbiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.DepositorInsert3xGFP11
UseCRISPRTagsMembrane Localization SignalExpressionMutationCodon optimizedPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-hfYFP-TEV-SAV
Plasmid#191197PurposeFusion of hfYFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purificationDepositorInserthfYFP & Streptavidin fusion
UseTags6xHis and Streptavidin core domain, amino acids 1…ExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-LSSmGFP-TEV-SAV
Plasmid#191200PurposeFusion of LSSmGFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purificationDepositorInsertLSSmGFP & Streptavidin fusion
UseTags6xHis and Streptavidin core domain, amino acids 1…ExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHC005 (∆RE)
Plasmid#226199PurposeConjugation knockout plasmid (pK18mobsacB) to delete H16_0006 (Type I restriction endonuclease subunit).DepositorInsertHomology arms for type I restriction-modification system restriction subunit H16_A0006
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX-6P-HMP1_FABD
Plasmid#186436PurposeExpresses the HMP1 F-actin Binding domain (677-927) with an N-terminal GST tag and a PRescission Protease cleavage siteDepositorInsertHMP1 (F-actin Binding Domain) (hmp-1 Nematode)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-OuIAD(W392A)
Plasmid#226980PurposeExpressing Olsenella uli indoleacetate decarboxylase (IAD) W392A variant in E. coliDepositorInsertOuIAD(W392A)
UseTagsHis6 (on backbone)ExpressionBacterialMutationW392APromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-OuIAD(W392F)
Plasmid#226979PurposeExpressing Olsenella uli indoleacetate decarboxylase (IAD) W392F variant in E. coliDepositorInsertOuIAD(W392F)
UseTagsHis6 (on backbone)ExpressionBacterialMutationW392FPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-OuIAD(F401A)
Plasmid#226978PurposeExpressing Olsenella uli indoleacetate decarboxylase (IAD) F401A variant in E. coliDepositorInsertOuIAD(F401A)
UseTagsHis6 (on backbone)ExpressionBacterialMutationF401APromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only