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Plasmid#65673PurposeExpresses NLS-CNL in mammalian cellsDepositorInsertthree copies of SV40 nuclear localization signal
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYNL-N1_3xNLS
Plasmid#65672PurposeExpresses NLS-YNL in mammalian cellsDepositorInsertthree copies of SV40 nuclear localization signal
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNUT N6His mICA NG
Plasmid#70162PurposeExpresses N-His tagged nonglycosylated mouse inhibitor of carbonic anhydraseDepositorInsertmouse inhibitor of carbonic anhydrase
UseTagsN-terminal signal peptide, 4 aa link, 6 His, Fact…ExpressionMammalianMutationAsn470Asp and Asn645AspPromoterSV40Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
p5A7-T
Plasmid#92020PurposeExpresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain and a second in the C-terminal domain, anhydrotetracycline inducible TEV proteaseDepositorInsertsTALE targeting lac operator with N and C-terminal TEV protease cleavage sites
Tobacco Etch Virus Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…ExpressionMutationS219VPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
p5A3-T
Plasmid#92019PurposeExpresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV proteaseDepositorInsertsTALE targeting lac operator with N-terminal TEV cleavage site
Tobacco Etch Virus Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…ExpressionMutationS219VPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
FCIV1-GR SS287AA/S155A
Plasmid#72700PurposeLentiviral expression of GR-2A phosphodeficient mutant (2nd generation)DepositorInsertGR (Nr3c1 Rat)
UseLentiviralTagsIRES-VenusExpressionMammalianMutationSS287AA/S155APromoterubiquitinAvailable sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_3xNLS
Plasmid#65674PurposeExpresses NLS-ONL in mammalian cellsDepositorInsertthree copies of SV40 nuclear localization signal
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL1855
Plasmid#53041PurposePLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st3):csrADepositorInsertsGFP
tetR
csrA
UseSynthetic BiologyTagsLVA, RBS(st3), and glgC leaderExpressionBacterialMutationPromoterpConNoHind and pLlac0-1Available sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEG610
Plasmid#40195DepositorInsertMex-5 (aa1-355) (RNAi resistant) (mex-5 Nematode)
UseTagsDendra2 PAFP (sequence optimized for worm express…ExpressionWormMutationcontains aa 1-355; exon 2 recoded to be RNAi-resi…Promoter4.4 kb mex-5 promoter fragmentAvailable sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
CA-0234 CMVTO-sumIL2-rensGS.3
Plasmid#231198PurposeCleaveIL-2DepositorInsertsumIL2-rensGS.3 (IL2 Human)
UseTagsExpressionMammalianMutationL80F, R81D, L85V, I86V, I92FPromoterAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
CA-0210 CMVTO-hIL2-rens-del.1
Plasmid#231227PurposeCleaveIL-2_loop1_delDepositorInserthIL2-rens-del.1 (IL2 Human)
UseTagsExpressionMammalianMutationL80F, R81D, L85V, I86V, I92FPromoterAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
CA-0211 CMVTO-hIL2-rens-del.2
Plasmid#231228PurposeCleaveIL-2_loop3_delDepositorInserthIL2-rens-del.2 (IL2 Human)
UseTagsExpressionMammalianMutationL80F, R81D, L85V, I86V, I92FPromoterAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
CA-0201 CMVTO-hIL6-rens.1
Plasmid#231197PurposeCleaveIL-6DepositorInserthIL6-rens.1 (IL6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
CA-0212 CMVTO-hIL2-rens-del.3
Plasmid#231229PurposeCleaveIL-2_loop2_delDepositorInserthIL2-rens-del.3 (IL2 Human)
UseTagsExpressionMammalianMutationL80F, R81D, L85V, I86V, I92FPromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-OuIAD(E502Q)
Plasmid#226973PurposeExpressing Olsenella uli indoleacetate decarboxylase (IAD) E502Q variant in E. coliDepositorInsertOuIAD(E502Q)
UseTagsHis6 (on backbone)ExpressionBacterialMutationE502QPromoterAvailable sinceJan. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
E547* FL USP7
Plasmid#229001PurposeE547-mutant of human USP7DepositorInsertUSP7 1-546 (USP7 Human)
UseTags6xHis cleavable with thrombinExpressionBacterialMutationE547 mutated to stop codonPromoterT7Available sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-4
Plasmid#228986PurposeFor bacterial expression of anti-GST nanobody GST-4, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-4
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-2
Plasmid#228984PurposeFor bacterial expression of anti-GST nanobody GST-2, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-2
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-GST-7
Plasmid#228989PurposeFor bacterial expression of anti-GST nanobody GST-7, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertGST-7
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaTdT-38
Plasmid#228979PurposeFor bacterial expression of anti-tdTomato nanobody LaTdT-38, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertLaTdT-38
UseTags6xHISExpressionBacterialMutationPromoterT7Available sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only