Addgene

We narrowed to 14,149 results for: LEA

Showing: 11521 - 11540 of 14149 results

Results per page:

LMX1B-gRNA-C-del

Plasmid

#131516

Purpose

gRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-N-del

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

LMX1B-gRNA-C-del

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
8xCTS1_WT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro_SV40polyA

Plasmid

#124546

Purpose

Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

Depositor

Tudor Fulga

Article

Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

Insert

WT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro

Use

Tags

Expression

Mammalian

Mutation

WT, WT

Promoter

Minimal ADE

Availability

Academic Institutions and Nonprofits only

Enlarge
pSR348

Plasmid

#124713

Purpose

Expression of narX under Ptac

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

narX (narX )
lacI (lacI )

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

S193* - LacI is truncated and likely deactivated.…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pBM019

Plasmid

#128179

Purpose

Construct for generating stable Cas9-expressing Toxoplasma gondii

Depositor

Sebastian Lourido

Article

Markus et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003

Inserts

sgRNA#1
SpCas9
Chloramphenicol acetyltransferase

Use

CRISPR; Toxoplasma gondii expression

Tags

3X FLAG and Nuclear Localization Signal

Expression

Mutation

Promoter

TUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29a-hNABP2

Plasmid

#128307

Purpose

Express hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

Human Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Y85C- please see depositor comments below

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pTrc99S-ssDsbA-MBP-4xDQNAT

Plasmid

#128398

Purpose

Periplasmic expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Maltose binding protein

Use

Tags

4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

Expression

Bacterial

Mutation

Y342H- please see depositor comments

Promoter

trc

Availability

Academic Institutions and Nonprofits only

Enlarge
BII-gR-PtW-eSpCas9

Plasmid

#133357

Purpose

Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

eSpCas9

Use

Tags

HA-tagged eSpCas9

Expression

Mammalian

Mutation

eSpCas9 mutations

Promoter

CMV enhancer and hEf1a (CpG free)

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA0055

Plasmid

#131774

Purpose

Guide RNA (gCASS5a) and Cas9 expression plasmid for cleaving pFA6 series deletion cassettes, including KanMX, hphMX and natMX. Used to perform CRISPR-Swap of alleles.

Depositor

Frank Albert

Article

Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

Insert

20mer CASS5a guide (gCASS5a) and 5' sgRNA

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA0057

Plasmid

#131784

Purpose

Guide RNA (gGFP) and Cas9 expression plasmid for cleaving pFA6 series GFP C-terminal tagging cassettes, including GFP-His3MX6. Used to perform CRISPR-Swap of alleles.

Depositor

Frank Albert

Article

Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

Insert

20mer GFP cassette guide (gGFP) and 5' sgRNA

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pTAMAHISTEV_PfeA

Plasmid

#128943

Purpose

expresses PfeA in E. coli

Depositor

James Naismith

Article

Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

Insert

ferric enterobactin receptor

Use

Tags

TEV protease cleavable 7xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed (1-75)

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-BaL-QES.i01.c08

Plasmid

#123214

Purpose

Mammalian expression plasmid for Env from the BaL HIV-1 isolate; QES mutant with enhanced binding to PG16

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Mutations T49D, P…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-25711-QES.c11

Plasmid

#123219

Purpose

Mammalian expression plasmid for Env from the 25711 HIV-1 isolate; QES mutant with enhanced binding to PG16

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (25711) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Mutations I181L, …

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-ZM197M-gp160-754*

Plasmid

#123220

Purpose

Mammalian expression plasmid for Env from the ZM197M HIV-1 isolate; C-terminal truncation

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (ZM197M) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Premature stop co…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-ZM197M-QES.c10

Plasmid

#123224

Purpose

Mammalian expression plasmid for Env from the ZM197M HIV-1 isolate; QES mutant with enhanced binding to PG16

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (ZM197M) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Mutations V208M, …

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-Q769gp160-754*

Plasmid

#123227

Purpose

Mammalian expression plasmid for Env from the Q769.d22 HIV-1 isolate; C-terminal truncation

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (Q769.d22) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Premature stop co…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-Q769-QES.i03.c04

Plasmid

#123229

Purpose

Mammalian expression plasmid for Env from the Q769.d22 HIV-1 isolate; QES mutant with enhanced binding to PG16

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (Q769.d22) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Mutations A200E, …

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-Q842gp160-754*

Plasmid

#123231

Purpose

Mammalian expression plasmid for Env from the Q842.d12 HIV-1 isolate; C-terminal truncation

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (Q842.d12) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Premature stop co…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-191084-gp160-754*

Plasmid

#123236

Purpose

Mammalian expression plasmid for Env from the 191084 B7-19 HIV-1 isolate; C-terminal truncation

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (191084 B7-19) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Premature stop co…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-191084-QES.i06.c07

Plasmid

#123239

Purpose

Mammalian expression plasmid for Env from the 191084 B7-19 HIV-1 isolate; QES mutant with enhanced binding to PG16

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (191084 B7-19) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Mutations P124D, …

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-DU422-QES.c12

Plasmid

#123250

Purpose

Mammalian expression plasmid for Env from the DU422 HIV-1 isolate; QES mutant with enhanced binding to PG16

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (DU422) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Mutations V181L, …

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge