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Plasmid#187187PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionBacterialMutationD34K, D87KPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phat2-dr-cPla2-C2-Y90F-V91F-EGFP-6H
Plasmid#187186PurposeProtein ExpressionDepositorInsertENSDARG00000024546 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionBacterialMutationY90F, V91FPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-719 CTLA4-GFP R70W
Plasmid#186124PurposeCTLA4 gene knockin with mutationDepositorInsertCTLA4 (CTLA4 Human)
UseCRISPRTagsExpressionMutationR70WPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-720 CTLA4-GFP R75W
Plasmid#186125PurposeCTLA4 gene knockin with mutationDepositorInsertCTLA4 (CTLA4 Human)
UseCRISPRTagsExpressionMutationR75WPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNL-CEF-IgG1-IRES-GFP
Plasmid#187008PurposeExpression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgG1DepositorInsertHuman anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgG1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNL-CEF-IgA1-IRES-GFP
Plasmid#187007PurposeExpression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1DepositorInsertHuman anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-cat-Caspase-1/4b
Plasmid#183366PurposeStable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-1/4b
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-chPKCdelta/eDHFR(69K6)-EGFP
Plasmid#178857PurposeMammalian expression of PKCδ-eDHFR(69K6) chimera fused to EGFPDepositorInsertPKCδ(1-229)-eDHFR(69K6)-PKCδ(280-675)-EGFP (PRKCD Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-EGFP-eDHFR(69K6)-cRaf
Plasmid#178849PurposeMammalian expression of cRaf fused to EGFP-eDHFR(69K6)DepositorInsertEGFP-eDHFR(69K6)-cRaf (RAF1 Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-M13-6His-KIF1C_P176L-GFP
Plasmid#185978PurposeBaculovirus expression of human KIF1C with P176L mutation and 6xHis and GFP tags.DepositorInsertKIF1C (KIF1C Human)
UseTags6xHis and eGFPExpressionInsectMutationP176L - mutation causing HSP (SPG58)PromoterAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GFP-IRES-mSNCA(1-95)
Plasmid#185716PurposeAAV expression of GFP and C-terminal truncated (1-95 amino acid) mouse α-Synuclein from hSyn promoterDepositorInsertsynuclein, alpha (Snca Mouse)
UseAAVTagsExpressionMutationdeleted AA 95-144PromoterhSynAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX neo PFKL-GFP, K272R
Plasmid#138290PurposeMammalian Expression, Lentiviral. Expression of human phosphofructokinase, liver isoform PFKL-GFP, K272R, fusion proteinDepositorInsertPFKL (PFKL Human)
UseLentiviralTagsGFPExpressionMammalianMutationK272RPromoterCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-shRIIα-RIIαS97A-IRES-EGFP
Plasmid#183457PurposeReplacement of endogenous rat PKA-RIIα subunits with the RIIα S97A variantDepositorInsertsgRNA and Prkar2a (Prkar2a Rat)
UseLentiviralTagsExpressionMutationshRNA-resistant mutations: C135>G, G138>A, …PromotershRNA: H1 / gene: ubiquitinAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-shRIIα-RIIαS97E-IRES-EGFP
Plasmid#183458PurposeReplacement of endogenous rat PKA-RIIα subunits with the RIIα S97E variantDepositorInsertsgRNA and Prkar2a (Prkar2a Rat)
UseLentiviralTagsExpressionMutationshRNA-resistant mutations: C135>G, G138>A, …PromotershRNA: H1 / gene: ubiquitinAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
p8130 MSCV GFP Puro C-FlagHA
Plasmid#184521PurposeExpression of GFPDepositorInsertGFP
UseRetroviralTagsFlag, HAExpressionMutationPromoterMSCV LTRAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-42-DExCon-antiGFPnanobody-mCherry-R11a
Plasmid#179902PurposeDonor with homologous arms for Rab11a to knock in DExCon-antiGFPnanobody-mCherry moduleDepositorInsertRAB11A, member RAS oncogene family (RAB11A Human)
UseCRISPR; Donor for homologous based knock inTagsantiGFPnanobody-mCherryExpressionBacterial and MammalianMutationPromoterTRE3GSAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-eSpCas9-2A-GFP-sgRNA-RDH11
Plasmid#161924PurposeTo generate RDH11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against RDH11 exon 2.DepositorInsertsgRNA targeting RDH11 exon 2
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot3-F818A-dsRNAres_Q
Plasmid#147262PurposeInsect Expression of DmNot3-F818A-dsRNAresDepositorInsertDmNot3-F818A-dsRNAres (Not3 Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-horse-Caspase-4
Plasmid#183380PurposeStable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-4
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-wombat-Caspase-4
Plasmid#183379PurposeStable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-4
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only