-
Plasmid#117500PurposeExpresses human Cdt1 fused to HA tagDepositorInsertCdc10-dependent transcript-1 (CDT1 1700 bp, Human)
UseTagsHAExpressionMammalianMutationwild-typePromoterCMVAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH0006
Plasmid#135448PurposeHuman expression vector containing Ubiquitous Chromatin Opening Element (UCOE) upstream of EF1alpha promoter, dCas9 that is fused to NLS, tagBFP and a KRAB domain.DepositorInsertdCas9-BFP-KRAB
UseLentiviralTagstagBFPExpressionMammalianMutationPromoterEF1AAvailable sinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-moxGFP-GOLGA2
Plasmid#207792PurposeDonor template for Puro-2A-moxGFP insertion into the N-terminus of the GOLGA2 locus for Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 Addgene #207791DepositorArticleInsertGOLGA2 Homology Arms flanking a Puro-moxGFP Cassette (GOLGA2 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-N-Puro-moxGFP-LMNB1
Plasmid#207773PurposeDonor template for Puro-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770DepositorArticleInsertLMNB1 Homology Arms flanking a Puro-moxGFP Cassette (LMNB1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
6xHis-TEV-ATG3 (Human autophagy E2-like enzyme)
Plasmid#169079PurposeHuman ATG3DepositorInsertATG3 (ATG3 Human)
UseTags6X Histidine Tag, TEV cleavage siteExpressionBacterialMutationPromoterT7 lac promoterAvailable sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXS-hs-HMGA2
Plasmid#52727Purposeretroviral expression of human HMGA2DepositorInsertHMGA2 (HMGA2 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAGIG-H2B-V5-mScarlet
Plasmid#191093PurposeTo express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsertH2B and mScarlet
UseAAVTagsH2B-V5-mScarletExpressionMammalianMutationThe fusion protein was optimized to the Human cod…PromoterCMV and CAGAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
GLuc-PM-Venus
Plasmid#164785PurposeGaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent proteinDepositorInsertGaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Gaussia princeps, Human)
UseTagsGLuc and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDYT002
Plasmid#186550PurposeLineage tracing vector (with barcoded Target Sites and triple sgRNAsDepositorInsert14-bp random integration barcode and three target sites
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mScarlet-LMNB1
Plasmid#207771PurposeDonor template for mScarlet insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770DepositorArticleInsertLMNB1 Homology Arms flanking a mScarlet Tag (LMNB1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNAI-GAL4-CREB
Plasmid#46769PurposeExpression of GAL4 fusion to wild type CREBDepositorUseTagsGAL4ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 TAK1/F
Plasmid#44161DepositorInsertTak1 (Map3k7 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAGImC_V5-CSNK1A1
Plasmid#92014PurposeExpresses V5-CSNK1A1, includes IRES-mCherryDepositorInsertCSNK1A1 (CSNK1A1 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-lamin A
Plasmid#175107PurposeLentiviral expression of V5-tagged mouse LmnaDepositorInsertLmna (Lmna Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUBC_stdMCP_serinemod_E488QADAR_p2A_yGFP
Plasmid#154787PurposeExpresses synonymized MS2 coat protein dimer (MCP) fused to hyperactive ADAR catalytic domainDepositorInsertAdenosine deaminse (Adar Fly, Synthetic)
UseLentiviralTagsGFP, V5, and stdMCPExpressionMammalianMutationSynonymized serine to prevent autoediting of ADAR…PromoterUBCAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 E-cadherin shRNA/E-cadherin GFP-IRES-HA-H-Ras V12
Plasmid#101285PurposeLentiviral expression ,was generatedby recombining bothIRES an HA-tagged H-Ras V12 into SbfI site of pLL5.0 Ecadherin shRNA/mEcad-GFPDepositorInsertE-Cadherin-GFP-H-Ras
UseLentiviralTagsGFPExpressionMammalianMutationH-Ras V12PromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FH-AGO2-WT
Plasmid#91978PurposeExpresses FLAG-HA-AGO2 (WT)DepositorInsertAGO2 (AGO2 Human)
UseLentiviralTagsFLAG-HAExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
NLS-PyronicSF/pCMV-myc-nuc
Plasmid#124814PurposeHighly resposive GFP-based pyruvate nanosensor with a nuclear localization signal.DepositorInsertPyronicSF
UseTagsNuclear Localization SignalExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-C-Fzd6
Plasmid#123589DepositorInsertfrizzled class receptor 6 (Fzd6 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
GABA(A) receptor subunit g2SE
Plasmid#49170PurposepHluorin-tagged GABA A receptor subunit (gamma 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surfaceDepositorInsertGABA(A) receptor subunit gamma 2 (Gabrg2 Mouse)
UseTagsmyc, pHGFP (pHluorin), and thrombin cleavage site…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only