Addgene

We narrowed to 14,149 results for: LEA

Showing: 11501 - 11520 of 14149 results

Results per page:

pAAV-EWB-DIO-myrTagRFP-T-P2A-post-eGRASP

Plasmid

#111581

Purpose

An AAV vector that expresses double floxed myristoylated TagRFP-T and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

myrTagRFP-T-P2A-post-eGRASP

Use

AAV

Tags

Expression

Mutation

Promoter

Ef1a

Availability

Academic Institutions and Nonprofits only

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pAAV-EWB-DIO-myriRFP670V5-P2A-post-eGRASP

Plasmid

#111585

Purpose

An AAV vector that expresses double floxed myristoylated iRFP670 with V5 tag and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

myriRFP670V5-P2A-post-eGRASP

Use

AAV

Tags

Expression

Mutation

Promoter

Ef1a

Availability

Academic Institutions and Nonprofits only

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pCEP4-Q769gp160-QES.i03.V255M

Plasmid

#111838

Purpose

Mammalian expression plasmid for Env from the Q769.d22 HIV-1 isolate; QES mutant with enhanced binding to antibody PG16

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (Q769.d22) Env

Use

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; mutations A200E;F…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCEP4-BaLgp160-QES.i01.c01

Plasmid

#111839

Purpose

Mammalian expression plasmid for Env from the BaL HIV-1 isolate; QES mutant with enhanced binding to PG16

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Use

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; mutations T49D;P1…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCEP4-DU422gp160-QES.c03

Plasmid

#111840

Purpose

Mammalian expression plasmid for Env from the DU422 HIV-1 isolate; QES mutant with enhanced binding to PG16

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (DU422.1) Env

Use

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; mutations V181L;V…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCEP4-25711gp160-QES.c02

Plasmid

#111842

Purpose

Mammalian expression plasmid for Env from the 25711 HIV-1 isolate; QES mutant with enhanced binding to PG16

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (25711.2) Env

Use

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; mutations I181L;V…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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N229A Lo-MetQ

Plasmid

#118268

Purpose

N229A Mutation in Methionine Binding Protein (MetQ) used to solve crystal structure

Depositor

Douglas Rees

Article

Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

Insert

MetQ (N229A) (metQ E. coli)

Use

Tags

10x His Tag and Enterokinase Cleavage Site

Expression

Bacterial

Mutation

Changed Asparagine (Asn) 229 to Alanine (Ala)

Promoter

T7 Promoter

Availability

Academic Institutions and Nonprofits only

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pLV-pGK-Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro

Plasmid

#115684

Purpose

NanoLuc-Firefly Luciferase fusion with constitutive nuclear expression for calibration of Nanoluc signal to Firefly Luciferase signal. H2B-mCherry marks chromatin with red fluorescence.

Depositor

David Suter

Article

Mandic et al Methods. 2017 May 1;120:65-75. doi: 10.1

Insert

Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCMV-sadCas9-VP64

Plasmid

#115790

Purpose

An AAV vector expressing S. aureus dCas9 fused to VP64.

Depositor

Nadav Ahituv

Article

Matharu et al Science. 2018 Dec 13. pii: science.aau06

Insert

Sa-dCas9

Use

AAV

Tags

NLS and NLS-VP64

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SP-His-mCherry-HRP-VhHGFP

Plasmid

#112157

Purpose

Expression and secretion of anti-GFP FLIPPER-bodies, small protein probes for correlated microscopy. FLIPPER-bodies are the fusion of a nanobody with a fluorescent protein and a peroxidase.

Depositor

Ben Giepmans

Article

de Beer et al Histochem Cell Biol. 2018 Mar;149(3):261

Insert

anti-GFP FLIPPER-body

Use

Tags

His and Thrombin cleavage site

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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SLF155: AAV-syn-DO-H2B-jRGECO1a

Plasmid

#112278

Purpose

For AAV based expression of nuclear jRGECO1a in neurons in Cre-negative neurons with FLEx switch

Depositor

Adam Cohen

Article

Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

Insert

H2B-jRGECO1a

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Availability

Academic Institutions and Nonprofits only

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NES-EGFP-cPHx2

Plasmid

#116854

Purpose

PI(3,4)P2 biosensor

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

PLEKHA1 (PLEKHA1 Human)

Use

Tags

EGFP and X.leavis map2k1.L(32-44)

Expression

Mammalian

Mutation

amino acids 169-329 (tandem dimer)

Promoter

Availability

Academic Institutions and Nonprofits only

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pET16b-PCNA

Plasmid

#134898

Purpose

Bacterial expression and purification of human PCNA

Depositor

Andrew Deans

Article

Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

Insert

PCNA (PCNA Human)

Use

Tags

untagged

Expression

Bacterial

Mutation

fully synthetic, codon optimized for bacterial ex…

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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GEF-H1 FLARE212.pa

Plasmid

#134930

Purpose

GEF-H1 biosensor that does not have GEF activity.

Depositor

Klaus Hahn

Article

Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097

Insert

GEF-H1(1-212)-yPet-4X linker-Cerulean3-GEF-H1(213-end)

Use

Tags

None.

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV and T7

Availability

Academic Institutions and Nonprofits only

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GEF-H1 FLARE191.pa

Plasmid

#134931

Purpose

GEF-H1 biosensor that has GEF activity.

Depositor

Klaus Hahn

Article

Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097

Insert

GEF-H1(1-191)-yPet-4X linker-Cerulean3-GEF-H1(192-end)

Use

Tags

None.

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV and T7

Availability

Academic Institutions and Nonprofits only

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p35S::IRE1a

Plasmid

#135232

Purpose

constitutive expression of IRE1a from A. thaliana (AT2G17520; upregulates UPR signaling)

Depositor

Armin Djamei

Article

Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

Insert

IRE1a (IRE1A Mustard Weed)

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Availability

Academic Institutions and Nonprofits only

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FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA

Plasmid

#137830

Purpose

MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal L190K mutant TEV protease, M cleavage sequence, and tTA transcription factor

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

Mutated Leucine 71 to Lysine in CTEVp fragment

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pET26b_Nb.AD101

Plasmid

#131402

Purpose

E. coli periplasmic expression of conformational nanobody Nb.AD101 recognizing purified active-state A2a adenosine receptor

Depositor

Andrew Kruse

Article

McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

Insert

Nanobody AD101

Use

Tags

6xHis-tag and pelB leader

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET26b_Nb.AD102

Plasmid

#131403

Purpose

E. coli periplasmic expression of conformational nanobody Nb.AD102 recognizing purified active-state A2a adenosine receptor

Depositor

Andrew Kruse

Article

McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

Insert

Nanobody AD102

Use

Tags

6xHis-tag and pelB leader

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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LMX1B-gRNA-C-del

Plasmid

#131516

Purpose

gRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-N-del

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

LMX1B-gRNA-C-del

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only