Showing: 11461 - 11480 of 14149 results
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Plasmid#67687PurposeUAS-driven 6xMyc nuclear localized tdTomato-2A-activated Dusp6 in minTol backbone; also with cry:cfp cassetteDepositorInsertdusp6 (dusp6 Zebrafish)
UseTags6xmyc-NLS-tdTomato-2AExpressionMutationS159A, S196APromoterAvailabilityAcademic Institutions and Nonprofits only -
pNvT-MHC::mCh
Plasmid#67943PurposeTransgenesis vector consisting of a 1.6-kb fragment of MyHC1 promoter region upstream of the start codon and an mCherry reporter gene, flanked by inverted binding sites of meganuclease I-SceIDepositorInsertmCherry
UseTransgenic reporter in the sea anemone, nematoste…TagsExpressionMutationPromoterMyosin Heavy Chain1 (MyHC1)AvailabilityAcademic Institutions and Nonprofits only -
mCardinal-NUP50-N-10
Plasmid#56170PurposeLocalization: Nuclear Pore, Excitation: 604, Emission: 659DepositorInsertNUP50 (NUP50 Human)
UseTagsmCardinalExpressionMammalianMutationPromoterCMVAvailabilityIndustry, Academic Institutions, and Nonprofits -
mIFP-Nup50-N-10
Plasmid#56233PurposeLocalization: Nuclear Pore, Excitation: 683, Emission: 704DepositorInsertNup50 (NUP50 Human)
UseTagsmIFPExpressionMammalianMutationPromoterCMVAvailabilityIndustry, Academic Institutions, and Nonprofits -
pEHIShqprt
Plasmid#92166PurposePlasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vectorDepositorInsertQuinolinate Phosphoribosyltransferase (QPRT Human)
UseTagsTEV protease -cleavable N-terminal 6HisExpressionBacterialMutationchanged Asp 2 to Asn to create BspHI sitePromoterT7AvailabilityAcademic Institutions and Nonprofits only -
VAMP8-mCherry
Plasmid#92424PurposeExpression of VAMP8 C-terminally conjugated to mCherry.DepositorInsertVAMP8 (Vamp8 Mouse)
UseTagsmCherryExpressionMammalianMutationA36S (Please see Depositor Comments below)PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCEP4-gp120(BaL)-CD4
Plasmid#98957PurposeMammalian expression plasmid for CD4-fused gp120 from HIV-1 BaL isolateDepositorInsertHIV-1 (BaL) gp120
UseTagsCD4 (domains D1-D2), CD5 leader sequence, FLAG ep…ExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCEP4-BaLgp160-QES.01
Plasmid#100921PurposeMammalian expression plasmid for Env from the BaL HIV-1 isolate; QES mutant with enhanced binding to PG16DepositorInsertHIV-1 (BaL) Env
UseTagsCD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic gene; mutations T49D;P1…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCEP4-BaLgp160-QES.02
Plasmid#100923PurposeMammalian expression plasmid for Env from the BaL HIV-1 isolate; QES mutant with enhanced binding to PG16DepositorInsertHIV-1 (BaL) Env
UseTagsCD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic gene; mutations T49D;P1…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-BaLgp140-8his
Plasmid#100924PurposeMammalian expression plasmid for soluble gp140 from the BaL HIV-1 strainDepositorInsertHIV-1 (BaL) Env
UseTagsCD5 leader sequence and Purification tag after D6…ExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-BaLgp120-8his
Plasmid#100925PurposeMammalian expression plasmid for gp120 subunit from the BaL HIV-1 strainDepositorInsertHIV-1 (BaL) Env
UseTagsCD5 leader sequence and Purification tag after K5…ExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Q769gp120-8his
Plasmid#100929PurposeMammalian expression plasmid for gp120 subunit from the Q769.d22 HIV-1 strainDepositorInsertHIV-1 (Q769.d22) Env
UseTagsCD5 leader sequence and Purification tag after K5…ExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCEP4-Q769gp160-QES.03
Plasmid#100930PurposeMammalian expression plasmid for Env from the Q769.d22 HIV-1 isolate; QES mutant with enhanced binding to antibody PG16DepositorInsertHIV-1 (Q769.d22) Env
UseTagsCD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic gene; mutations A200E;F…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Q842gp120-8his
Plasmid#100932PurposeMammalian expression plasmid for gp120 subunit from the Q842.d12 HIV-1 strainDepositorInsertHIV-1 (Q842.d12) Env
UseTagsCD5 leader sequence and Purification tag after K5…ExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCEP4-Q842gp160-QES.04
Plasmid#100933PurposeMammalian expression plasmid for Env from the Q842.d12 HIV-1 isolate; QES mutant with enhanced binding to antibody PG16DepositorInsertHIV-1 (Q842.d12) Env
UseTagsCD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic gene; mutations P124D;F…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLenti_CamKIIa_mKate2
Plasmid#96942PurposeTo label mature excitatory iPSC-derived human neuronsDepositorInsertfar-red fluorescent protein
UseLentiviralTagsExpressionMutationE87D (please see depositor comments below)PromoterCaMKIIaAvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1T2
Plasmid#97328PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include two additional T residues shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1T2
UseTags6xHis - TEVExpressionBacterialMutationAddition of two T residues after T25PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
Sa-dCas9-NLS-3xFLAG/pcDNA3.1
Plasmid#98041PurposeExpresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis to purify specific genomic regions of interestDepositorInsertSa-dCas9-NLS-3xFLAG
UseCRISPRTags3xFLAG tag and NLS (nuclear localization signal)ExpressionMammalianMutationD10A + H557APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pJ414-PCY1:S495A
Plasmid#98151PurposeExpresses PCY1:S495A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.DepositorInsertPeptide cyclase 1:S495A
UseTagsHis6-TEVExpressionBacterialMutationChanged serine 495 to alaninePromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pJ414-PCY1:W603A
Plasmid#98153PurposeExpresses PCY1:W603A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.DepositorInsertPeptide cyclase 1:W603A
UseTagsHis6-TEVExpressionBacterialMutationChanged tryptophan 603 to alaninePromoterT7AvailabilityAcademic Institutions and Nonprofits only
Showing: 11461 - 11480 of 14149 results