Addgene

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7Gli:GFP

Plasmid

#110494

Purpose

reporter of Hedgehog signaling activity

Depositor

Michael Lewis

Article

Li et al PLoS Genet. 2016 May 19;12(5):e1006054.

Insert

7 repeats of the Gli binding site GAACACCCA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

7 repeats of Gli biding site

Available since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-uvsY

Plasmid

#163914

Purpose

Expresses uvsY for bacterial expression and affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

uvsY (uvsY Enterobacteria phage T4)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCGN-HCF-1 fl

Plasmid

#53309

Purpose

plasmid contains human HCFC1 full length gene with HA and c-myc tags

Depositor

Winship Herr

Article

Wilson et al Cell. 1993 Jul 16;74(1):115-25.

Insert

HCFC1 (HCFC1 Human)

Use

Tags

HA and c-myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 28, 2014

Availability

Academic Institutions and Nonprofits only

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pCGN-HCF-1 fl

Plasmid

#53309

Purpose

plasmid contains human HCFC1 full length gene with HA and c-myc tags

Depositor

Winship Herr

Article

Wilson et al Cell. 1993 Jul 16;74(1):115-25.

Insert

HCFC1 (HCFC1 Human)

Use

Tags

HA and c-myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 28, 2014

Availability

Academic Institutions and Nonprofits only

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WFS1-Flag-pcDNA3

Plasmid

#13011

Depositor

Fumihiko Urano

Article

Fonseca et al J Biol Chem. 2005 Nov 25. 280(47):39609-

Insert

Wolfram Syndrome 1 (WFS1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2006

Availability

Academic Institutions and Nonprofits only

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WFS1-Flag-pcDNA3

Plasmid

#13011

Depositor

Fumihiko Urano

Article

Fonseca et al J Biol Chem. 2005 Nov 25. 280(47):39609-

Insert

Wolfram Syndrome 1 (WFS1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2006

Availability

Academic Institutions and Nonprofits only

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PB-UniSAM

Plasmid

#99866

Purpose

Encodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0

Depositor

Lesley Forrester

Article

Fidanza et al Sci Rep. 2017 Jul 25;7(1):6394. doi: 10.

Insert

UniSAM-mCherry + U6-gRNA2.0

Use

CRISPR and Synthetic Biology; Dcas9-sam activation

Tags

Expression

Mammalian

Mutation

BbsI sites in CDS were ablated by consensus mutat…

Promoter

EF1a

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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PB-UniSAM

Plasmid

#99866

Purpose

Encodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0

Depositor

Lesley Forrester

Article

Fidanza et al Sci Rep. 2017 Jul 25;7(1):6394. doi: 10.

Insert

UniSAM-mCherry + U6-gRNA2.0

Use

CRISPR and Synthetic Biology; Dcas9-sam activation

Tags

Expression

Mammalian

Mutation

BbsI sites in CDS were ablated by consensus mutat…

Promoter

EF1a

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLENTI6.3/AR-GC-E2325

Plasmid

#85128

Purpose

exogenous AR expression

Depositor

Karl-Henning Kalland

Article

Olsen et al BMC Cancer. 2016 Jul 4;16:377. doi: 10.1

Insert

androgen receptor (AR Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pLENTI6.3/AR-GC-E2325

Plasmid

#85128

Purpose

exogenous AR expression

Depositor

Karl-Henning Kalland

Article

Olsen et al BMC Cancer. 2016 Jul 4;16:377. doi: 10.1

Insert

androgen receptor (AR Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEVS104

Plasmid

#207393

Purpose

Conjugal helper plasmid

Depositor

Jason Peters, Edward Ruby, Eric Stabb

Article

Hall et al MicroPubl Biol. 2023 Jul 13;2023:10.1791

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Shp2 biosensor

Plasmid

#134346

Purpose

FRET biosensor. To visualize Shp2 activity by FRET

Depositor

Yingxiao Wang

Article

Sun et al Nat Commun. 2017 Sep 7;8(1):477. doi: 10

Insert

Shp2 Biosensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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Shp2 biosensor

Plasmid

#134346

Purpose

FRET biosensor. To visualize Shp2 activity by FRET

Depositor

Yingxiao Wang

Article

Sun et al Nat Commun. 2017 Sep 7;8(1):477. doi: 10

Insert

Shp2 Biosensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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luxB:cp157Venus+luxA/pRSETB Duet

Plasmid

#197583

Purpose

BRET lux luciferase with cp157Venus for bacterial expression

Depositor

Takeharu Nagai

Article

Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

Insert

LuxA LuxBcp157Venus

Use

E.coli expression

Tags

His X 6

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLJC5-LAMP1-RFP-3xHA

Plasmid

#102932

Purpose

Express a lysosomal Tag

Depositor

David Sabatini

Article

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

LAMP1

Use

Lentiviral

Tags

RFP-HA

Expression

Mutation

Promoter

ubc

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pLJC5-LAMP1-RFP-3xHA

Plasmid

#102932

Purpose

Express a lysosomal Tag

Depositor

David Sabatini

Article

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

LAMP1

Use

Lentiviral

Tags

RFP-HA

Expression

Mutation

Promoter

ubc

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pWXC078

Plasmid

#222961

Purpose

C. elegans codon-optimized mScarlet-I3 with 3 introns cloned into pPD95.75 backbone

Depositor

Oliver Hobert

Article

Cao et al bioRxiv 2024.06.11.598534

Insert

mScarlet-I3

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAJ075_hsCRBN

Plasmid

#124214

Purpose

Insect cell expression vector for His6 tagged hsCRBN

Depositor

Eric Fischer

Article

Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

Insert

Cereblon (CRBN Human)

Use

Tags

His6-TEV

Expression

Insect

Mutation

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAJ075_hsCRBN

Plasmid

#124214

Purpose

Insect cell expression vector for His6 tagged hsCRBN

Depositor

Eric Fischer

Article

Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

Insert

Cereblon (CRBN Human)

Use

Tags

His6-TEV

Expression

Insect

Mutation

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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Human Lipid Droplet and Metabolism Library

Pooled Library

#191535

Purpose

Lentiviral CRISPR library targeting 1,196 genes related to lipid droplet biology and cell metabolism.

Depositor

James Olzmann

Article

Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

Expression

Mammalian

Use

CRISPR and Lentiviral

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only