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Plasmid#125071PurposeExpression of SO_4387 under PtacDepositorUseSynthetic BiologyTagsExpressionBacterialMutationS286L- Please see depositor comments belowPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only
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pET-28a-His6-SUMO-VAMP2
Plasmid#135551PurposeExpress mouse VAMP2 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertVAMP2
UseTags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_STf_LamB1
Plasmid#181999PurposeCMV overexpression of SNAP-tag fast fusion to Lamin B1 for nuclear envelope localization in mammalian cell linesDepositorInsertSTf-LamB1
UseTagsExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-alphaCaMKII-E2-Crimson-3XNLS-pA
Plasmid#183803PurposeAAV vector to express nuclear localized E2-Crimson from CaMKIIa promoterDepositorInsertE2-Crimson-NLS
UseAAVTagsNLSExpressionMutationPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_ygiF-CHAD
Plasmid#127793PurposeExpresses the CHAD domain of ygiF in E. coli cellsDepositorInsertygiF-CHAD (ygiF Escherichia coli)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationN-terminal TTM domain of ygiF (residues 201-433)PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-ZM197M-gp160
Plasmid#123226PurposeMammalian expression plasmid for Env from the ZM197M HIV-1 isolateDepositorInsertHIV-1 (ZM197M) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS-foxc1a-2a-nlstdTomato
Plasmid#132970Purposefull-length of foxc1a in frame with viral 2a peptide and nuclear localized tdTomato in pCS expression vectorDepositorInsertsUseTagsNLS and mycExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046940
Plasmid#149261PurposeBacterial expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;StrepIIExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
AQTFAATHNPWASQA_epitope
Plasmid#165019PurposeExpression of CMV UL86 (1222-1236) fused to CD74DepositorInsertCMV UL86 (1222-1236)
UseLentiviralTagsCD74 at N-term; cathepsin S cleavage site (GRWHTV…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
MCP-ADAR2-DD(E488Q, N496F)
Plasmid#170139PurposeAAV vector carrying the MCP-ADAR2-DD(E488Q, N496F) with a nuclear export signalDepositorInsertNES-MCP-ADAR2-DD(E488Q, N496F)
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJ414-PCY1
Plasmid#98150PurposeExpresses PCY1 from Saponaria vaccaria possessing a N-terminal His6-tag (TEV cleavable) in E. coli.DepositorInsertPeptide cyclase 1
UseTagsHis6-TEVExpressionBacterialMutationWTPromoterT7Available sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-ZapCY2
Plasmid#59016PurposeCytosolic zinc-binding FRET biosensorDepositorInsertNES-ZapCY2 genetically encoded cytosolic Zn(II) sensor
UseTagsNuclear Exclusion SignalExpressionMammalianMutationSee commentPromoterCMVAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSFFV-dNme2Cas9
Plasmid#193190PurposeLentiviral vector expressing nuclease-dead Cas9 from Neisseria meningitis (dNme2)DepositorInsertdNme2Cas9
UseTagsNLS and NLS-3xHA-NLSExpressionMammalianMutationH588A, D16APromoterSFFVAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
PRK5-FLAG-USP13/5-6
Plasmid#61748Purposeexpress N-terminally FLAG tagged human chimera USP13/5-6DepositorUseTagsFLAGExpressionMammalianMutationUSP13 (1-721aa) fused to USP5 (717-859aa); D23Y i…PromoterCMVAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP ratAPOBEC1 L173A/G227A
Plasmid#167169PurposeExpresses rat APOBEC1 L173A/G227A in mammalian cells. Please note that this does not express GFP.DepositorArticleInsertAPOBEC1 (Apobec1 Rat)
UseTagsExpressionMammalianMutationchanged Leucine 173 to Alanine; changed Glycine 2…PromoterCMV promoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ER-CaNARi
Plasmid#64852PurposeEndoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of ER membrane.DepositorInsertCYP450-CaNARi
UseTagsCerulean, N-terminal 27 amino acids from CYP450, …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28b – E. coli RecE residues 564-868
Plasmid#104534PurposeExpresses E. coli RecE residues 564-868DepositorInsertRecE residues 564-866 (C-terminal nuclease domain)
UseTags6X-His tag followed by thrombin siteExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEY68
Plasmid#191065Purposeceh-14 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInsertceh-14 (ceh-14 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-zfKitlgb
Plasmid#168200PurposeExpress zebrafish Kitlgb, mRNA synthesisDepositorInsertKitlgb (kitlgb Zebrafish)
UseVertebrate expression (zebrafish, chicken, xenopu…TagsExpressionMammalianMutationI85V, V222I, and D233N- please see depositor comm…PromoterAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Claspin-Flag
Plasmid#136246PurposeProtein expression of Claspin with a C terminal FLAG tagDepositorInsertClaspin
UseTagsFlagExpressionMammalianMutationE263G, Q499R, I783T, D932G and S1062G- Please see…PromoterAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only