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Plasmid#68606PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsNuclear localization signal-tagged Citrine (Citri…ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only
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pcDNA3.1(+)-blueAKAR(T/A)
Plasmid#118471PurposeNegative-control mutant for blueAKAR biosensor.DepositorInsertblueAKAR(T/A)
UseTags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable sinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBP-T7_RBS-His6-Thrombin
Plasmid#72979PurposeT7 promoter, RBS, His6-tag and thrombin cleave site - derived from pET15b (5' CTAT/3' CATA) fusion)DepositorInsertT7+RBS+His6+thrombin
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
nuc-yHS1
Plasmid#159166PurposeNuclear labile heme reporterDepositorInsertnuc-yHS1
UseTagsSV40 NLSExpressionYeastMutationPromoterGPDAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
NES-EGFP-cPHx1
Plasmid#116853PurposePI(3,4)P2 biosensorDepositorInsertPLEKHA1 (PLEKHA1 )
UseTagsEGFP and X.leavis map2k1.L(32-44)ExpressionMammalianMutationAmino acids 169.329PromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-NLS
Plasmid#51571PurposeConstitutively express nuclear localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsNLS and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-bArrestin2-TevC-P2A-TdTomato
Plasmid#89869Purposeexpresses iTango bArrestin2-TevC component and TdTomato bicistronicallyDepositorInsertbArrestin2-TevC-P2A-TdTomato
UseSynthetic BiologyTagsExpressionMammalianMutationR173H in TdTomato (please see depositor comments …PromoterCMVAvailable sinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-Htb2
Plasmid#51569PurposeConstitutively express nuclear localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsHTB2 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-Bsa4-ICL1
Plasmid#105822PurposeExpression of Mycobacterium tuberculosis icl1 recombinant proteinDepositorInserticl1
UseTagsHis tag with TEV cleavageExpressionBacterialMutationPromoterT7Available sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEY79
Plasmid#191074Purposeflp-12 fluorescent neural reporter driving nuclear T-Sapphire expression (refer to NeuroPAL paper for expression)DepositorInsertflp-12 (flp-12 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEY62
Plasmid#191020Purposeacr-5 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorInsertacr-5 (acr-5 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.U6gLacZ.hSyn.flex.H2B.RFP
Plasmid#170373PurposeNegative control guide, Cre-inducible plasmid expressing nuclear RFP.DepositorInsertCre inducible H2B RFP
UseAAVTagsExpressionMutationPromoterhuman SynapsinAvailable sinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_R472A_H477A
Plasmid#83483PurposeBacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 1 inactive mutant).DepositorInsertLbu_C2c2_R472A_H477A
UseCRISPRTagsHis6-MBPExpressionBacterialMutationR472A, H477APromoterAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSL1180-HR-PUbEGFP-NLS
Plasmid#176681PurposeExpression of nuclear localized EGFP under Ae. aegypti Polyubiquitin promoter (AAEL003888), EGFP version of pSL1180-HR-PUbECFP (Addgene #47917)DepositorInsertEGFP
UseTagsExpressionInsectMutationPromoterAe. aegypti poly-ubiquitin (AAEL003888)Available sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
3xFNLDD/pMXs-puro
Plasmid#49536PurposeExpresses 3xFNLDD in mammalian cellsDepositorInsert3xFNLDD
UseRetroviralTags3xFLAG tag and NLS (nuclear localization signal)ExpressionMammalianMutationPromoterLTRAvailable sinceDec. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_DiCre_59.F2
Plasmid#60207PurposeExpresses a fusion protein of FKBP12-linker peptide 2- Cre(19-59)DepositorInsertCre recombinase (CD207 Human, Synthetic, Bacteriophage P1)
UseTagsFKBP12 and Nuclear localisation signalExpressionMammalianMutationamino acids 19-59 of the humanized version of Cre…PromoterCMVAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Flag-hDcp2
Plasmid#72213Purposemammalian expression of Flag-tagged human Dcp2DepositorInsertDcp2 (DCP2 Human)
UseTagsFlagExpressionMammalianMutationQ170R (please see depositor's comment below)PromoterCMVAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-nl.EGFP-tdTomato
Plasmid#172382PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertnuclear-localized EGFP + tdTomato
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
UAS:bloSwitch
Plasmid#108355PurposeUAS driven green to red switch reporter for B3 recombinase expressionDepositorInsertUAS:[Ubiquitin-C-intron]-[blown-out site]-nuclear-GFP-[beta-globin-polyA]-[blown out site]-[membrane TagRFPT]
UseZebrafish expressionTagsExpressionMutationZebrafish codon optimizedPromoterlacAvailable sinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-BirA(R118G)-YFP-NLS-STOP-L2
Plasmid#127360Purposegateway entry vector for expressing nuclear BirA* (R118G)-YFP under a promoter of choiceDepositorInsertBirA* (R118G mutant)
UseGateway entry vectorTagsGS linker, NLS, V5, and YFPExpressionMutationR118GPromoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only