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JUP_pLX307
Plasmid#98347PurposeLentiviral expression of JUPDepositorInsertJUP (JUP Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterE1FaAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBT281
Plasmid#122611Purposeexpress evoAPOBEC1-BE4max in mammalian cellsDepositorInsertevoAPOBEC1-BE4max
UseTagsExpressionMammalianMutationE4K H109N H122L D124N R154H A165S P201S F205SPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_GLP1R-NTEV-TCS-GV-2xHA
Plasmid#194378PurposeSplit TEV assaysDepositorInsertGLP1R-NTEV-TCS-GV-2xHA (GLP1R Human, Synthetic)
UseTags2xHAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBECKP-spe
Plasmid#117236Purposencas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickaseDepositorInsertncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCB301TOR-GFP
Plasmid#164799PurposeExpression of GFP in oomycetes using Agrobacterium-mediated transformationDepositorInsertGFP
UseOomycete expressionTagsExpressionMutationPromoterAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-Lifeact_Halo_T2A_EGFP
Plasmid#135445PurposeMamalian cell expression of HaloTag7 localized at F-actin and cytosolic EGFPDepositorInsertLifact-HaloTag7 and EGFP
UseTagsLifactExpressionMammalianMutationPromoterCMV/TetOAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLS-SV40-mP-Rluc
Plasmid#106292PurposeLuciferase lentivirus based enhancer assay vectorDepositorInsertLuciferase
UseLentiviral and LuciferaseTagsExpressionMutationPromoterDerived from pGL4.23Available sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-flag-GFP-NCAM-1
Plasmid#205190PurposeThis vector encodes of a synCAM (NCAM1) and GFPDepositorInsertsynCAM NCAM1 and GFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMCB306-GFP-Rab12
Plasmid#198471Purposelentiviral expression of GFP-Rab12DepositorInsertGFP-Rab12 (RAB12 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterEF1AAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
LRTi
Plasmid#214072PurposeLuminescent reporter system to measure EJC-enhanced NMD activity. No intron in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationLinker sequence present between firefly luciferas…PromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC0079 RESCUE-S
Plasmid#130662PurposepCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375A/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/S661T) V16SDepositorInsertdRanCas13b-ADAR2dd
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC590 - pCMV ER-iRFP
Plasmid#59136PurposeA plasmid that express endoplasmic reticulum-targeted iRFP under the CMV promoter.DepositorInsertER-localized Infrared Fluorescent Protein
UseTagsER retention signal and signal peptideExpressionMammalianMutationPromoterCMV-IEAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SIGLEC7-Fc(DAPA)-AviTag-6xHis
Plasmid#156773PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertSIGLEC7 (SIGLEC7 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-RUNX2
Plasmid#192926PurposeBarcoded piggybac transposon vector with Dox-inducible expression of RUNX2DepositorInsertRUNX2 (RUNX2 Human)
UseTagsHAExpressionMammalianMutationPromoterTET3G-miniCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTDpelB-NTwinStrep
Plasmid#45940Purposeuse to create N-terminal PelB-Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal PelB-Twin-Strep-tag and C-terminal 6x His-tag for periplasmic translocation via PelB signal sequenceDepositorTypeEmpty backboneUseTagsHIS-6-tag, PelB export signal, and Twin-Strep-tag…ExpressionBacterialMutationPromoterlacIq-PtrcAvailable sinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
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AbVec2.0-mIghg1
Plasmid#127158PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG1 isotypeDepositorInsertIghg1 (Ighg1 Mouse)
UseTagsExpressionMammalianMutationPromoterhCMV IE1 gene promoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSV40-mCherry-P2A-Hygro
Plasmid#106478PurposeExpression of mCherry and Hygromycin resistance gene from SV40 promoterDepositorInsertsmCherry
Hygromycin B resistance
UseTagsExpressionMammalian and PlantMutationPromoterSV40 promoterAvailable sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-FlaviA-mNeptune-puro
Plasmid#140091PurposeFluorescence activatable reporter of flavivirus NS2B-NS3 protease activityDepositorInsertFlaviA-mNeptune
UseLentiviral; High expressionTagsmNeptuneExpressionMammalianMutationPromoterCMVAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only
