-
Plasmid#197146PurposeExpresses ApoE2 in Mammalian cellsDepositorInsertApoE2 (APOE Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
C321.∆A.opt
Bacterial Strain#87359PurposeThis is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.DepositorBacterial ResistanceGentamicinSpeciesSyntheticAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPolI_SARS-CoV-2_NLuc
Plasmid#183953PurposeEncodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoterDepositorUseTagsExpressionMammalianMutationPromoterMammalian Pol IAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-IRESWT-Gbetagamma ONE-GO
Plasmid#204339PurposeGPCR/G protein BRET biosensorDepositorInsertGbetagamma ONE-GO
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Chr8q_Centromere-Targeting_gRNA
Plasmid#195128PurposegRNA targeting centromere-proximal location on Chromosome 8q in a third generation Cas9 backbone with GFPDepositorInsertChr8q gRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA
Plasmid#20299PurposeCre-activated AAV expression of mCherryDepositorInsertmCherry
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-CD4-myc
Plasmid#58537PurposeExpresses human CD4 with a C-terminal myc tag in mammalian cellsDepositorInsertCD4 (CD4 Human)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GFP DNAJB9
Plasmid#19505DepositorInsertDNAJB9 (DNAJB9 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pT7 LasI
Plasmid#193631PurposeConstitutive T7 RNAP-mediated expression of LasI 3OC12-HSL synthase for Las quorum sensing system. LasI sequence is codon optimized for E. coli.DepositorInsertLasI
UseTags6xHisExpressionBacterialMutationPromoterT7 RNAP promoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
His6TEV-UbG76C
Plasmid#75299PurposeBacteria expression plasmid for Ubiquitin G76C with removable His6 tag by TEV proteaseDepositorInserthuman Ubiquitin mutant G76 to C
UseTagsHisExpressionBacterialMutationchanged Glycine 76 to CystinePromoterT7Available sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv2(Gaussia Luciferase)#5 in pTwist-CMV
Plasmid#216164PurposeExpression of secreted Gaussia Luciferase specifically in cells with TDP-43 loss of functionDepositorInsertGaussia princeps luciferase (with double mutation to improve stability)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX313-TP53-WT
Plasmid#118014PurposeConstitutive expression of wild-type human Tumor Protein p53 (TP53)DepositorInsertTP53 (TP53 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJL1-SpCas9
Plasmid#117051PurposeIn vitro expression of S. pyogenes Cas9 from the T7 promoterDepositorInsertS. pyogenes Cas9
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_XPO1_WT
Plasmid#81812PurposeGateway Donor vector containing XPO1 , part of the Target Accelerator Plasmid Collection.DepositorInsertXPO1 (XPO1 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)
Plasmid#175573PurposeInactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repressionDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKD4-Nter-APEX2
Plasmid#112868PurposeBacterial plasmid derived from pKD4 for chromosomal insertion of APEX2 gene in 5' of a gene of interest by using Datsenko & Wanner methodDepositorInsertAPEX2
UseTagsExpressionMutationPromoterAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV_BE4max_3xHA
Plasmid#112096PurposeC:G-to-T:A base editingDepositorInsertBE4max_3xHA
UseCRISPRTags3xHAExpressionMammalianMutationPromoterCMVAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO-shBRCA1 #2
Plasmid#44595DepositorInsertBRCA1 (Brca1 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
LC-E03
Bacterial Strain#78552PurposeE. coli strain K-12 MG1655, pTet--Cas9 cassette integrated at 186 primary attB site.DepositorBacterial ResistanceNoneAvailable sinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Flag-SPRTN-wt
Plasmid#110214PurposeExpression in mammalian cells of full-length wild-type SPRTN protein with a Flag-tag at N-terminus.DepositorInsertSPRTN (SPRTN Human)
UseTagsExpressionMammalianMutationP296L (see depositor comments below)PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only