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Plasmid#177205PurposePermuted td intron with evolved initiation sequence for the expression of circular mRNADepositorInsertPermuted GFP with evolved initiation sequence for circular mRNA
UseTags6x HisExpressionBacterialMutationPermuted sfGFP after residue 52 & Changed Cyt…PromoteraraBADAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:(MCS)-3xHA-S11
Plasmid#108180PurposeBinary vectors used for the inducible expression of tripartite split-sfGFP components (with a 3xHA tag) in plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:S10-(MCS)-3xHA
Plasmid#108178PurposeBinary vectors used for the inducible expression of tripartite split-sfGFP components (with a 3xHA tag) in plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
P16ΔS:GFP1–9
Plasmid#108186PurposeBinary vectors used for the constitutive expression of tripartite split-sfGFP components (GFP1-9) in plantsDepositorInsertGFP1-9
UseSynthetic BiologyTagsExpressionPlantMutationPromoterP16ΔSAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNP1 OR input A
Plasmid#107365PurposeInput A for pNP1 OR gate sfGFPDepositorInsertOR gate input A
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNP1 OR input B
Plasmid#107366PurposeInput B for pNP1 OR gate sfGFPDepositorInsertOR gate input B
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNP1 AND input A
Plasmid#107362PurposeInput A for pNP1 AND gate sfGFPDepositorInsertAND gate input A
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNP1 AND input B
Plasmid#107363PurposeInput B for pNP1 AND gate sfGFPDepositorInsertAND gate input B
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKC52
Plasmid#226780PurposeExpresses Drosophila UNC-104(R348G) in insect cellsDepositorInsertDrosophila unc-104 (R348G) (unc-104 Fly)
UseTagssfGFP and 2xStrepIIExpressionInsectMutationchanged Arginine 348 to GlycinePromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKC50
Plasmid#226778PurposeExpresses Drosophila UNC-104(V6M) in insect cellsDepositorInsertDrosophila unc-104 (V6M) (unc-104 Fly)
UseTagssfGFP and 2xStrepIIExpressionInsectMutationchanged Valine 6 to MethioninePromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pKC31
Plasmid#226777PurposeExpresses Drosophila UNC-104(R562H) in insect cellsDepositorInsertDrosophila unc-104 (R562H) (unc-104 Fly)
UseTagssfGFP and 2xStrepIIExpressionInsectMutationchanged Arginine 562 to Histidine (bris mutation)PromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pKC51
Plasmid#226779PurposeExpresses Drosophila UNC-104(A255V) in insect cellsDepositorInsertDrosophila unc-104 (A255V) (unc-104 Fly)
UseTagssfGFP and 2xStrepIIExpressionInsectMutationchanged Alanine 255 to ValinePromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLP3
Plasmid#209958PurposeEntry vector to store Level 0 broad-host range L. plantarum replication origin partsDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYN2_1
Plasmid#184757PurposePlasmid for genome editing by CRISPR/Cas9DepositorInsertsCas9
sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme
UseSynthetic BiologyTags2xNLSExpressionBacterial and YeastMutationPromoterPGK1 and tRNA-Phe-HDV RibozymeAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRMTK-clo39
Plasmid#204014PurposeCloning plasmid containing the sfGFP insert with a chloramphenicol resistance markerDepositorInsertsuper folder green fluorescent protein
UseTagsExpressionPlantMutationPromoterN/AAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pASPIre4
Plasmid#196656PurposeDerivative of pASPIre3. Contains SpeI restriction site within the CDS of bxb1 to enable diversification of the 5’-UTR and codons 2-16.DepositorInsertBxb1-sfGFP fusion controlled by rhamnose promoter; attB/attP-flanked discriminator; exchangable 5'-UTR and CDS (codons 1-16)
UseTagsExpressionBacterialMutationSpeI site in Bxb1 CDSPromoterrhamnose-inducible promoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 ΔGRM-ΔC
Plasmid#112659PurposeExpression S.cerevisiaeDepositorInsertGFP-Glo3 ΔGRM ΔC
UseTagssfGFPExpressionYeastMutationΔ376-493 (deletion of Glo3 motif & C-terminal…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 S389,398 D
Plasmid#112661PurposeExpression S.cerevisiaeDepositorInsertGFP-Glo3 S389,398D
UseTagssfGFPExpressionYeastMutationmutations (S389D, S398D) in Glo3 motif, phosphomi…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only