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Plasmid#68555PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only
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pG108-K - AhpC-Glow
Plasmid#185135PurposeBacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers BS2 is cloned under the Bacteroides thetaiotaomicron ahpC promoterDepositorInsertsGlow gene
Bacteroides thetaiotaomicron ahpC promoter
UsePg106TagsExpressionBacterialMutationPromoterAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSR0
Plasmid#161984PurposeAllele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile ribotype 027 and other ribotypes. Uses inducible expression of CD2517.1 toxin as a counter-selection markerDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMADM-beta
Plasmid#36891DepositorInsertsBeta-Geo
thymidine kinase
UseTagsExpressionMammalianMutationPromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpySrtA
Plasmid#51139Purposeexpression plasmid for N-terminal his tagged S.pyogenes SrtADepositorInsertS.pyogenes SrtA
UseTags6x HisExpressionBacterialMutationDeleted amino acids 1-80PromoterT7Available sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pLV 4xSTAR-sTomato-NLS-blast
Plasmid#136259PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 4 repeats
UseLentiviralTagssTomato-NLSExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSR
Plasmid#161983PurposeAllele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile 630. Uses inducible expression of CD2517.1 toxin as a counter-selection markerDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
6x-His_A1aY1_GFP
Plasmid#158753PurposeBacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purificationDepositorInsert6xHistidine_A1aY1_GFP
UseTags6x-Histidine tag, GFP, T7 leader sequence, and Th…ExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
hSyn1-tdTomatof
Plasmid#104060PurposeAlso known as hSyn1-tdTomato-f. AAV expression of Farnylsated tdtomato for tracingDepositorInserttdTomato
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV 4xSTAR-mScarletI-NLS-blast
Plasmid#136260PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 4 repeats
UseLentiviralTagsmScarletI-NLSExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-eNpHR 3.0-EYFP
Plasmid#26775Purpose3rd gen lentiviral expression of eNpHR 3.0 fused to EYFP driven by human Synapsin I promoter for optogenetic inhibitionDepositorInserteNpHR 3.0
UseLentiviralTagsEYFPExpressionMammalianMutationTrafficking Signal (TS) ER Export SignalPromoterhSynAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-DYRK1B_IDG-K
Plasmid#158183PurposeGateway destination clone of DYRK1B (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-DYRK1B (DYRK1B Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
plk1 WT GFP-myc
Plasmid#132963PurposeTo study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damageDepositorInsertpolo-like kinase 1 (PLK1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT/Caggs-NRAS G12V-IRES-ASK1
Plasmid#221074PurposeExpresses NRAS G12V and ASK1 in mammalian cellsDepositorInsertsUseTagsExpressionMammalianMutationG12VPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV 4xSTAR-mNeonGreen-NLS-blast
Plasmid#136258PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 4 repeats
UseLentiviralTagsmNeonGreen-NLSExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
hIRE1a K599A
Plasmid#20745DepositorInsertIRE1 (ERN1 Human)
UseLentiviral; Destination vectorTagsExpressionMammalianMutationK599A, dominant negative kinase mutantPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_EGFP_m6A_Tracer
Plasmid#139403Purposein vitro transcription for mRNADepositorInsertm6A-Tracer
UseIn vitro transcriptionTagsEGFPExpressionMutationPromoterT3Available sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-BRSK2-V5-TurboID_IDG-K
Plasmid#135253PurposeGateway destination clone of BRSK2 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertBRSK2-V5-TurboID (BRSK2 Human)
UseLentiviral; Gateway destinationTagsV5-TurboIDExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-CDK12-V5_IDG-K
Plasmid#135245PurposeGateway destination clone of CDK12 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertCDK12-V5 (CDK12 Human)
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only