Addgene

We narrowed to 24,661 results for: cat

Showing: 11341 - 11360 of 24661 results

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pRSFDuet1-His-TEV-OTC_d1_Ub

Plasmid

#170729

Purpose

Scaﬀold design for studying ubiquitin interacting proteins by cryo-EM.

Depositor

Shang-Te Danny Hsu

Article

Chiu et al Biochemistry. 2021 Apr 13;60(14):1075-10

Insert

E. coli ornithine transcarbamylase (OTC), fused to human ubiquitin

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

The C-terminus of OTC was truncated by removing o…

Promoter

T7

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-Flag-stm2585_L139*

Plasmid

#174400

Purpose

S. Typhimurium gene stm2585 (sarA/steE) truncated after 139th amino acid, which removes the stretch homologous to gp130

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Use

Tags

EGFP and Flag

Expression

Mammalian

Mutation

L139* (Truncation after 139th amino acid in sarA)

Promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMHE-bNup98-DN-NLS-mClover3

Plasmid

#171495

Purpose

T7 promotor drives in vitro transcription of mClover3-tagged bovine Nup98 DN mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

Nup98-DN (NUP98 B. tarus (bovine))

Use

Pgehme

Tags

mClover3

Expression

Mutation

Promoter

T7

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pFL GST-UBXN2A

Plasmid

#169016

Purpose

Expresses GST-UBXN2A (human) in insect cells

Depositor

Hemmo Meyer

Article

Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

Insert

UBXN2A (UBXN2A Human)

Use

Tags

GST

Expression

Insect

Mutation

Promoter

polyhedrin promoter

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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Ash1-5p

Plasmid

#169712

Purpose

Bacterial Expression of truncated yeast Ash1 including encoding amino acids 420-480 with phosphorylation sites 429 and 470 removed

Depositor

Tanja Mittag

Article

Martin et al J Am Chem Soc. 2016 Nov 30;138(47):15323

Insert

Ash1 (ASH1 Budding Yeast)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Truncation including encoding amino acids 420-480…

Promoter

T7

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pWZL N-Myc-hPOT1_K90E-IRES_hygro

Plasmid

#166407

Purpose

Express hPOT1 K90E in mammalian cells

Depositor

Agnel Sfeir

Article

Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

Insert

hPOT1 (POT1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

K90E

Promoter

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCK-D390(UniProt)-FLAG

Plasmid

#164692

Purpose

Express N-terminally truncated Drosha-FLAG in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

N-terminally truncated Drosha (DROSHA Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnma1a

Plasmid

#164951

Purpose

Zebrafish kcnma1a gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnma1a (kcnma1a Zebrafish)

Use

Pcr cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET15-His-∆NGlpG(C104A/M247C)

Plasmid

#155133

Purpose

Expresses N-terminally His-tagged E. coli GlpG C104A/M247C variant (residues 87-276) from pET15b

Depositor

Sinisa Urban

Article

Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

Insert

GlpG (glpG Escherichia coli)

Use

Tags

His

Expression

Bacterial

Mutation

C104A/M247C, insert codes for GlpG residues 87-276

Promoter

T7

Available since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-XYLT2-sgRNA

Plasmid

#154862

Purpose

Lentiviral expression of Cas9 and sgRNA targeting XYLT2

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

XYLT2 sgRNA (XYLT2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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D78_Ld_362280

Plasmid

#135891

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

transmembrane emp24 domain-containing protein

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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D86_Ld_260650

Plasmid

#135899

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

Protein of unknown function (DUF2012), putative

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Ctcf-R

Plasmid

#122320

Purpose

Expresses sgRNA targeting mouse Ctcf and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Ctcf

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Kdm1a-L

Plasmid

#122327

Purpose

Expresses sgRNA targeting mouse Kdm1a and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Kdm1a

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-mHdac1-R2

Plasmid

#122308

Purpose

Expresses sgRNA targeting mouse Hdac1 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Hdac1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCas9n-Nanog-L

Plasmid

#122302

Purpose

Expresses sgRNA targeting mouse Nanog and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Nanog (Nanog Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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SNX17-PX (1-111)

Plasmid

#119098

Purpose

Bacterial expression of human phox homology (PX) domain, SNX17-PX (1-111)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX17-PX (1-111) (SNX17 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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SNX16-PX (104-216)

Plasmid

#119097

Purpose

Bacterial expression of human phox homology (PX) domain, SNX16-PX (104-216)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX16-PX (104-216) (SNX16 Human)

Use

Tags

Expression

Mutation

Promoter

N/A

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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SNX4-PX (48-186)

Plasmid

#119085

Purpose

Bacterial expression of human phox homology (PX) domain, SNX4-PX (48-186)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX4-PX (48-186) (SNX4 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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SNX32-PX (17-166)

Plasmid

#119111

Purpose

Bacterial expression of human phox homology (PX) domain, SNX32-PX (17-166)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX32-PX (17-166) (SNX32 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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