We narrowed to 16,198 results for: grna

1. pLKO.1-TST-EGFP-GPIBY55

   Plasmid

   #213707

   Purpose

   For lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIBY55 driven by an hPGK promoter.

   Depositor

   Qilai Huang

   Article

   Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

   Insert

   EGFP
   

   Tags

   Twin-strep-tag

   Expression

   Mammalian

   Promoter

   hPGK

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC57-ITR2-H1- sgCTG Merry-eCMV-SaSpD10A+linker (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #210736

   Purpose

   Coding for SaSp D10A Cas9 alongside Merry sgRNA targeting CAG repeats

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Inserts

   SaSp D10A Cas9
   Merry sgCAG
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, PolyA signal

   Expression

   Mammalian

   Mutation

   D10A, N-terminal Sa Cas9, C-terminal Sp Cas9

   Promoter

   H1 and eCMV

   Available Since

   Jan. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC57-ITR2-H1- sgCTG Gollum-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #210735

   Purpose

   Coding for SaSp D10A Cas9 alongside Gollum sgRNA targeting CAG repeats

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Inserts

   SaSp D10A Cas9
   Gollum sgCAG
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, PolyA signal

   Expression

   Mammalian

   Mutation

   D10A, N terminal Sa Cas9, C-terminal Sp Cas9

   Promoter

   H1 and eCMV

   Available Since

   Jan. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. hLig4 guide2 in pX458

   Plasmid

   #211536

   Purpose

   sgRNA-2 against Lig4

   Depositor

   Agnel Sfeir

   Article

   Brambati et al Science. 2023 Aug 11;381(6658):653-660.

   Insert

   sgRNA hLig4 (LIG4 Synthetic)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   u6

   Available Since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. hLig4 guide1 in pX458

   Plasmid

   #211535

   Purpose

   sgRNA-1 against Lig4

   Depositor

   Agnel Sfeir

   Article

   Brambati et al Science. 2023 Aug 11;381(6658):653-660.

   Insert

   sgRNA hLig4 (LIG4 Synthetic)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   u6

   Available Since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiCRISPR-v2 hygro sgUCK1

   Plasmid

   #211524

   Purpose

   Deletes UCK1

   Depositor

   Richard Possemato

   Article

   Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

   Insert

   sgUCK1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAf-CRISPR-ctfR1

   Plasmid

   #207992

   Purpose

   CRISPR vector used with pAf-CRISPR-phoA (#207991) to delete both aflatoxin and cyclopiazonic acid gene clusters of Aspergillus flavus

   Depositor

   Perng-Kuang Chang

   Article

   Chang et al Fungal Genet Biol. 2023 Dec 27;170:10386

   Insert

   ctfR1
   

   Use

   CRISPR

   Promoter

   Aspergillus flavus U6

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACBE-ASR (pXK-607)

   Plasmid

   #208822

   Purpose

   Universal Antibiotic Screening Reporter for adenine and cytosine base editors. Key Construct: CMV-PuroR*(ATAACG)-eGFP-hU6-gRNA.

   Depositor

   Kun Xu

   Article

   Ma et al J Biol Chem. 2022 Jul;298(7):102103. doi

   Type

   Empty backbone

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV, U6

   Available Since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACBE-FSR (pXK-1648)

   Plasmid

   #208821

   Purpose

   Universal Fluorescent Surrogate Reporter for adenine and cytosine base editors. Key Construct: CMV-mCherry-CMV-eGFP* (ATAACG)-hU6-gRNA.

   Depositor

   Kun Xu

   Article

   Ma et al J Biol Chem. 2022 Jul;298(7):102103. doi

   Type

   Empty backbone

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV, U6

   Available Since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB-scrCtnnd2.1-mCherry-CAAX

    Plasmid

    #209108

    Purpose

    Membrane tethered mCherry scramble of shRNA targeting Ctnnd2 N-terminus

    Depositor

    Cagla Eroglu

    Article

    Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

    Insert

    scrCtnnd2.1
    

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPB-shCtnnd2.1-mCherry-CAAX

    Plasmid

    #209107

    Purpose

    Membrane tethered mCherry shRNA targeting Ctnnd2 N-terminus

    Depositor

    Cagla Eroglu

    Article

    Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

    Insert

    shCtnnd2.1
    

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLKO.1-shCdh20.1 GFP

    Plasmid

    #209105

    Purpose

    GFP expressing shRNA targeting Cdh20

    Depositor

    Cagla Eroglu

    Article

    Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

    Insert

    shCdh20.1
    

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLKO.1-shCdh10.1 GFP

    Plasmid

    #209101

    Purpose

    GFP expressing shRNA targeting Cdh10

    Depositor

    Cagla Eroglu

    Article

    Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

    Insert

    shCdh10.1
    

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1-shCtnnd2.2-GFP

    Plasmid

    #209097

    Purpose

    GFP expressing shRNA targeting Ctnnd2 3' UTR

    Depositor

    Cagla Eroglu

    Article

    Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

    Insert

    shCtnnd2.2
    

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLKO.1-scrCtnnd2.1-GFP

    Plasmid

    #209096

    Purpose

    GFP expressing scramble of shRNA targeting Ctnnd2 N terminus

    Depositor

    Cagla Eroglu

    Article

    Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

    Insert

    scrCtnnd2.1
    

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKE011

    Plasmid

    #207912

    Purpose

    Level 0 for gRNA in C4 module (5' GCAG-3' TCAG)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU3-AarI-scaffold-term
    

    Use

    CRISPR

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCS018

    Plasmid

    #207911

    Purpose

    Level 0 for gRNA in C3 module (5' TTCG-3' GCAG)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU6-AarI-scaffold-term
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCS017

    Plasmid

    #207909

    Purpose

    Level 0 for gRNA in C1 module (5' GGCT-3' AGCC)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU6-AarI-scaffold-term
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCS020

    Plasmid

    #207915

    Purpose

    Level 0 for gRNA in D3 module (5' TCCC-3' CTGC)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU6-AarI-scaffold-term
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKE012

    Plasmid

    #207914

    Purpose

    Level 0 for gRNA in AD2 module (5' TGAC-3' TCCC)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU3-AarI-scaffold-term
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge