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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,248 results for: grna

Showing: 11321 - 11340 of 16248 results

  1. p305N-4 (nonfunctional)

    Plasmid
    #246287
    Purpose
    Evaluation of AtU6.1c1 promoter (nonfunctional) (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line
    Depositor
    Chung-Jui Tsai
    Article
    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294
    Insert
    AtU6.1c1 promoter (nonfunctional)
    Use
    CRISPR
    Expression
    Plant
    Mutation
    G-to-C at -15 from TSS
    Promoter
    AtU6.1c1 promoter (nonfunctional)
    Available Since
    Oct. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    246287-plasmid-map-sequence-id-491143

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  2. p305N-20

    Plasmid
    #246303
    Purpose
    Evaluation of MtU6.6-189 promoter (Pol III promoter) deletion (189 bp) for CRISPR-Cas9 editing of mEGFP in Nicotiana benthamiana and poplar reporter lines
    Depositor
    Chung-Jui Tsai
    Article
    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294
    Insert
    MtU6.6-189 promoter
    Use
    CRISPR
    Expression
    Plant
    Promoter
    MtU6.6-189 promoter
    Available Since
    Oct. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    246303-plasmid-map-sequence-id-491106

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  3. p305N-16

    Plasmid
    #246299
    Purpose
    Evaluation of HbU6.2m1 promoter (Pol III promoter) sequence variation for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line
    Depositor
    Chung-Jui Tsai
    Article
    Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294
    Insert
    HbU6.2m1 promoter
    Use
    CRISPR
    Expression
    Plant
    Mutation
    A-to-G (-56 from TSS), C-to-T (-29), G-to-A (-24)
    Promoter
    HbU6.2m1 promoter
    Available Since
    Oct. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    246299-plasmid-map-sequence-id-491103

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  4. pAAV-hsynapsin-DIO-adra2a-shRNA-mCherry

    Plasmid
    #245063
    Purpose
    AAV-transgene knocking down adra2a receptor (adrenergic 2a receptor) transcripts cell type-selectively. Using the pPRIME. system, this generates mir30-derived shRNAs and a marker from the same RNA.
    Depositor
    William Wisden
    Insert
    AAV-human synapsin promoter-DIO-adra2a-shRNA-mCherry (Adra2a Mouse, Synthetic)
    Use
    AAV, Cre/Lox, and RNAi
    Promoter
    human synapsin
    Available Since
    Oct. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    245063-plasmid-map-sequence-id-489355

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  8. ADEPT-pTarget-GFP

    Plasmid
    #238041
    Purpose
    Encodes sfGFP under lac promoter. Expresses a single guide RNA (under Rha promoter), which targets a noncoding region of the plasmid as part of the ADEPT system. Carries oriT and kan resistance.
    Depositor
    Lingchong You
    Article
    Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/
    Insert
    sfGFP
    Use
    Synthetic Biology
    Promoter
    lac
    Available Since
    June 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    238041-plasmid-map-sequence-id-476072

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  9. ADEPT-pTarget-RFP

    Plasmid
    #238040
    Purpose
    Encodes mRFP1 under pLux promoter. Expresses a single guide RNA (under Ara promoter), which targets a noncoding region of the plasmid as part of the ADEPT system. Carries oriT and kan resistance.
    Depositor
    Lingchong You
    Article
    Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/
    Insert
    mRFP1
    Use
    Synthetic Biology
    Promoter
    Lux
    Available Since
    June 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    238040-plasmid-map-sequence-id-476070

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  17. ADEPT-pTarget

    Plasmid
    #238033
    Purpose
    Encodes sfGFP-SsrA under constitutive promoter (J23119). Expresses a single guide RNA, which targets a noncoding region of the plasmid as part of the ADEPT system. Carries oriT and kan resistance.
    Depositor
    Lingchong You
    Article
    Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/
    Insert
    sfGFP
    Use
    Synthetic Biology
    Tags
    ssrA
    Promoter
    J23119 (constitutive)
    Available Since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    238033-plasmid-map-sequence-id-471747

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  20. pYPQAT02

    Plasmid
    #223374
    Purpose
    T-DNA vector for SpCas9 mediated mutagenesis for plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-SpCas9-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223374-plasmid-map-sequence-id-452305

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Showing: 11321 - 11340 of 16248 results