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Plasmid#123222PurposeMammalian expression plasmid for Env from the ZM197M HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16DepositorInsertHIV-1 (ZM197M) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; Premature stop co…PromoterCMVAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-Q842-QES.i04.c05
Plasmid#123233PurposeMammalian expression plasmid for Env from the Q842.d12 HIV-1 isolate; QES mutant with enhanced binding to PG16DepositorInsertHIV-1 (Q842.d12) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; Mutations P124D, …PromoterCMVAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-BaL-QES.i01.c08-754*
Plasmid#123213PurposeMammalian expression plasmid for Env from the BaL HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16DepositorInsertHIV-1 (BaL) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; Premature stop co…PromoterCMVAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SHIV327C-HuA10-QES.i09.c07
Plasmid#123269PurposeMammalian expression plasmid for Env from the SHIV327C-Hu A10 strain; QES mutant for enhanced presentation of quaternary epitopesDepositorInsertSHIV327C-Hu A10 Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; Mutations V208M, …PromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-DU422-QES.c12-754*
Plasmid#123249PurposeMammalian expression plasmid for Env from the DU422 HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16DepositorInsertHIV-1 (DU422) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; Premature stop co…PromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-B41gp160-V200E-754*
Plasmid#123244PurposeMammalian expression plasmid for Env from the B41 HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16DepositorInsertHIV-1 (B41) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; Premature stop co…PromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-191084-QES.i06.c07-754*
Plasmid#123238PurposeMammalian expression plasmid for Env from the 191084 B7-19 HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16DepositorInsertHIV-1 (191084 B7-19) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; Premature stop co…PromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-25711-QES.c11-754*
Plasmid#123218PurposeMammalian expression plasmid for Env from the 25711 HIV-1 isolate; C-terminal truncation; QES mutant with enhanced binding to PG16DepositorInsertHIV-1 (25711) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; Premature stop co…PromoterCMVAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-GFP-Atg17(416) (L313D I314K)
Plasmid#121409PurposeExpressing GFP-Atg17DepositorInsertCUP1p-GFP-ATG17 ORF-CYC1 terminator
UseTagsExpressionYeastMutationLeucine 313 to Aspartic acid; Isoleucine 314 to …PromoterCUP1 promoterAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-Q842gp160-QES.i04.I181L
Plasmid#111837PurposeMammalian expression plasmid for Env from the Q842.d12 HIV-1 isolate; QES mutant with enhanced binding to antibody PG16DepositorInsertHIV-1 (Q842.d12) Env
UseTagsCD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic gene; mutations P124D;I…PromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR3-vsv-CenpB 1-166-dCEN-Incenp-GFP
Plasmid#108486Purposeinducible expression of vsv-Mis12-dCEN-INCENP-EGFPDepositorInsertCenpB-dCEN-INCENP (INCENP Human)
UseTagsEGFP and VSVExpressionMammalianMutationCEN box replaced by CenpB 1-166PromoterCMVAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-HF2-BE3
Plasmid#120397PurposeExpresses HF2-BE3 in mammalian cellsDepositorInsertHF2-BE3
UseTagsExpressionMammalianMutationMammalian codon-optimized Cas9-HFPromoterCMVAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-KSR R615H
Plasmid#118332PurposeExpression of mouse KSRDepositorInsertKSR R615H (Ksr1 Mouse)
UseTagsFlagExpressionMammalianMutationR615HPromoterAvailable sinceNov. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-Cα(S11D)-mPAGFP
Plasmid#114135PurposeMammalian expression of the S11D mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertthe mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11D mutation (Prkaca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_m-AKAR5
Plasmid#114139PurposeMammalian expression of the AKAR5 sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInsertAKAR5 C-terminally tagged by KRas C tail
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_cyt-AKAR5
Plasmid#114138PurposeMammalian expression of the AKAR5 sensor tagged with nucleus export signal (NES) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInsertAKAR5 with C-terminal nuclear export signal (NES)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-Cα(S11A)-mPAGFP
Plasmid#114134PurposeMammalian expression of the S11A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertthe mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11A mutation (Prkaca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN-mPAGFP
Plasmid#114114PurposeMammalian expression of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertthe N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP (Prkaca Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFL-SV40-TAK1 5'-UTR
Plasmid#115356Purposefirefly luciferase (Fluc) reporter containing the 5’-UTR of TAK1 mRNADepositorInsertTAK1 5'-UTR (MAP3K7 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1-M4
Plasmid#114036PurposeExpression of HEC1 M4 mutant tagged with GFPDepositorInsertGFP tagged - HEC1 -M4 (NDC80 Human)
UseTagsExpressionMammalianMutationM4 (K146E, R153E, K156E)PromoterCMV TetOAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only