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Plasmid#182515PurposeC-SWAT compatible vector for C-terminal tagging with AID*-6FLAG for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.DepositorInsertAID*
UseTags6FLAGExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT IAA17-6FLAG
Plasmid#182516PurposeC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration.DepositorInsertIAA17
UseTags6FLAGExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT IAA17-3HA
Plasmid#182517PurposeC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin inducible degron) and 3HA. ARF16(PB1)-P2A-osTIR1 with SIR4 homology arms for genome integration.DepositorInsertIAA17
UseTags3HAExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT IAA17-3MYC
Plasmid#182518PurposeC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin inducible degron) and 3MYC. ARF16(PB1)-P2A-osTIR1 with SIR4 homology arms for genome integration.DepositorInsertIAA17
UseTags3MYCExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCZGY2748
Plasmid#193331PurposeSite specific CRISPR/Cas9 editing of C. elegans Chr IDepositorInsertsgRNA for ttTi4348
UseTagsNoneExpressionWormMutationPromoterU6Available sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRPS5A-PTP-3Xflag-16S rRNA Left_G1397C-ABE
Plasmid#189642Purpose16S rRNA Left_G1397C-ABEDepositorInsertTALE and DddAtox and TadA 8e
UseTALENTagsExpressionPlantMutationPromoterRPS5AAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
KEE
Plasmid#187419PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationPromoterPH and p10Available sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
p09008_AF1-4+GFP5-7
Plasmid#173715PurposePlasmid backbone for the HyperXpress workflow containing new to nature hybrid GFP with strong fluorescence in E. coli cell free protein synthesis extracts.DepositorInsertshybrid GFP
mKate2
UseE. coli cell free protein synthesis extractsTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
SNG
Plasmid#187424PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationPromoterPH and p10Available sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
OST
Plasmid#187422PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationPromoterPH and p10Available sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSiMPls_N + pSiMPls_C
Plasmid#167203PurposeSiMPl plasmid pair (pSiMPls) for use with spectinomycin/streptomycinDepositorInsertsEGFP
SmR 1-75 + gp41-1 N-intein
mRuby3
gp41-1 C-intein + SmR 76-263
UseTagsExpressionBacterialMutationPromoterAmpR, CAT, pBAD, and pTrcAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
TEG
Plasmid#187421PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationPromoterPH and p10Available sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
FER
Plasmid#187418PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationPromoterPH and p10Available sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
CHI
Plasmid#187417PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationPromoterPH and p10Available sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2412
Plasmid#175965PurposeGenetic complementation plasmid of LetAB with 6PLL3-hairpinDepositorInsertsLetA
LetB
UseTagsExpressionBacterialMutationΔ701-710, replace with 347-354PromoterT7Available sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2268
Plasmid#175963PurposeGenetic complementation plasmid of LetA-[LetB PLL3->6] mutantDepositorInsertsLetA
LetB
UseTagsExpressionBacterialMutationΔ347-361, replace with 702-716PromoterT7Available sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2409
Plasmid#175964PurposeGenetic complementation plasmid of LetAB with 6PLL3-helixDepositorInsertsLetA
LetB
UseTagsExpressionBacterialMutationΔ711-715, replace with 355-359PromoterT7Available sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBA2705
Plasmid#189998PurposeInducible expression of FBP75(1–200)-VhhGFP4, integrate at 177 bp, phleomycin marker (deGradFP for cytoplasmic proteins)DepositorInsertsFBP75
VhhGFP4
UseExpression vector for trypanosoma bruceiTagsExpressionMutationN-terminal 600 bp of FBP75PromoterAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBA2675
Plasmid#189997PurposeInducible expression of FBP75 (1–200)-NLS-VhhGFP4, integrate at 177 bp, phleomycin marker (deGradFP for nuclear proteins)DepositorInsertsFBP75
VhhGFP4
UseExpression vector for trypanosoma bruceiTagsNLSExpressionMutationN-terminal 600 bp of FBP75PromoterAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBlueScript II SK-SNAP donor for Hist1h3g
Plasmid#186932PurposeGenomic targeting of SNAP tag at Hist1h3g C-terminalDepositorInsertSNAP donor for Hist1h3g (H3c8 Mouse)
UseTagsSNAPExpressionMammalianMutationPromoterT7Available sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only