Skip to main content
Addgene

pBA2705
(Plasmid #189998)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 189998 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBA310 (Derivative of pDEX777)
  • Backbone size w/o insert (bp) 5601
  • Total vector size (bp) 6597
  • Vector type
    Expression vector for Trypanosoma brucei
  • Selectable markers
    phleomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    FBP75
  • Alt name
    Tb927.5.700
  • Species
    Trypanosoma brucei
  • Insert Size (bp)
    600
  • Mutation
    N-terminal 600 bp of FBP75
  • GenBank ID
    AAZ11185.1

Gene/Insert 2

  • Gene/Insert name
    VhhGFP4
  • Species
    Synthetic
  • Insert Size (bp)
    345

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The insert DNA was gene synthesized by GeneArt

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBA2705 was a gift from Bungo Akiyoshi (Addgene plasmid # 189998 ; http://n2t.net/addgene:189998 ; RRID:Addgene_189998)
  • For your References section:

    Targeted protein degradation using deGradFP in Trypanosoma brucei. Ishii M, Akiyoshi B. Wellcome Open Res. 2022 Jun 23;7:175. doi: 10.12688/wellcomeopenres.17964.1. eCollection 2022. 10.12688/wellcomeopenres.17964.1 PubMed 35865221