pBA2705
(Plasmid
#189998)
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PurposeInducible expression of FBP75(1–200)-VhhGFP4, integrate at 177 bp, phleomycin marker (deGradFP for cytoplasmic proteins)
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 189998 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBA310 (Derivative of pDEX777)
- Backbone size w/o insert (bp) 5601
- Total vector size (bp) 6597
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Vector typeExpression vector for Trypanosoma brucei
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Selectable markersphleomycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameFBP75
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Alt nameTb927.5.700
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SpeciesTrypanosoma brucei
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Insert Size (bp)600
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MutationN-terminal 600 bp of FBP75
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GenBank IDAAZ11185.1
Gene/Insert 2
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Gene/Insert nameVhhGFP4
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SpeciesSynthetic
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Insert Size (bp)345
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe insert DNA was gene synthesized by GeneArt
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBA2705 was a gift from Bungo Akiyoshi (Addgene plasmid # 189998 ; http://n2t.net/addgene:189998 ; RRID:Addgene_189998) -
For your References section:
Targeted protein degradation using deGradFP in Trypanosoma brucei. Ishii M, Akiyoshi B. Wellcome Open Res. 2022 Jun 23;7:175. doi: 10.12688/wellcomeopenres.17964.1. eCollection 2022. 10.12688/wellcomeopenres.17964.1 PubMed 35865221