Addgene

We narrowed to 17,065 results for: gateway

Showing: 11261 - 11280 of 17065 results

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pZA13

Plasmid

#158491

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 without STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1

Use

Golden gate entry vector

Tags

Expression

Mutation

no STOP codon

Promoter

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ1360 - Intron GG3 - smu-1

Plasmid

#159806

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG3 - smu-1

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ1358 - Intron GG1 - smu-1

Plasmid

#159804

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG1 - smu-1

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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NHA- Delta 5

Plasmid

#36440

Depositor

Edward Chan

Article

Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

Insert

TNRC6A Delta 5 (TNRC6A Human)

Use

Tags

NHA

Expression

Mammalian

Mutation

aa 1670-1962 only

Promoter

Available since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
Khc73-GFP

Plasmid

#31746

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Khc73 (Khc-73 Fly)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Metallothionein

Available since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only
pET-DEST42-SARA-V5-His6

Plasmid

#67431

Purpose

Expression of hSara with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Use

Tags

His and V5

Expression

Bacterial

Mutation

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 GTF2B

Plasmid

#67067

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

GTF2B (GTF2B Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
SIM2_pCSdest

Plasmid

#53790

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

SIM2 (SIM2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

Sept. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pME-QF2w-SV40pA

Plasmid

#155117

Purpose

Middle entry vector containing QF2w

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QF2w

Use

Gateway middle entry vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pFS337-USP44-FLAG

Plasmid

#43905

Depositor

Stephen Elledge

Article

Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

Insert

USP44 (USP44 Human)

Use

Baculoviral expression

Tags

FLAG

Expression

Insect

Mutation

Promoter

Available since

April 1, 2013

Availability

Academic Institutions and Nonprofits only
pCFJ2214 - Intron GG1 - 900 bp

Plasmid

#159880

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG1 - 900 bp

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCPP3417

Plasmid

#128724

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopY1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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D. vulgaris Cas5c/pHMGWA

Plasmid

#81182

Purpose

Expresses Desulfovibrio vulgaris Cas5c in E. coli with TEV-cleavable N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

Insert

Cas5c

Use

CRISPR

Tags

6xHis - MBP - TEV protease site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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D. vulgaris Cas8c/pHMGWA

Plasmid

#81183

Purpose

Expresses Desulfovibrio vulgaris Cas8c in E. coli with TEV-cleavable N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

Insert

Cas8c

Use

CRISPR

Tags

6xHis - MBP - TEV protease site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCellFree_G06_ZIKV PrM

Plasmid

#145350

Purpose

Cell-free expression vector for Zika virus prM protein with C-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV PrM

Use

Tags

mCherry and His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Ad-shMKP-3

Plasmid

#131530

Purpose

Silence the MKP-3 expression

Depositor

Haiyan Xu

Article

Jiao et al PLoS One. 2012;7(7):e41168. doi: 10.1371

Insert

Short hairpin mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)

Use

Adenoviral

Tags

No

Expression

Mutation

Promoter

Available since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-MXC

Plasmid

#37140

Depositor

Robert Duronio

Article

White et al J Cell Biol. 2011 May 16;193(4):677-94.

Insert

Multi Sex combs (mxc Fly)

Use

Gateway

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2012

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 IFNG

Plasmid

#67056

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

IFNG (IFNG Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
MAC_N_TEAD2

Plasmid

#167804

Purpose

Expresses TEAD2 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

TEAD2 (TEAD2 Human)

Use

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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RSPH1_pCSdest

Plasmid

#53926

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

RSPH1 (RSPH1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits