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Plasmid#91816PurposeBacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with an R1282H mutationDepositorInsertSPT6 (SPT6 Budding Yeast)
UseTags6xHisExpressionBacterialMutationchanged arginine 1282 to histidine; deleted resid…PromoterT7Available sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3495 (pET151_Spt6 1247-1451 K1435A)
Plasmid#91817PurposeBacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with a K1435A mutationDepositorInsertSPT6 (SPT6 Budding Yeast)
UseTags6xHisExpressionBacterialMutationchanged lysine 1435 to alanine; deleted residues …PromoterT7Available sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX459-sgPLXNB2
Plasmid#86151PurposeCas9/CRISPR plasmid for cut in human PLXNB2 gene exon 3DepositorInsertPlexin-B2 (PLXNB2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shCdc20
Plasmid#160954PurposeCdc20 shRNA in pMKO.1 retroviral vectorDepositorInsertCdc20 shRNA (Cdc20 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shKif11
Plasmid#160962PurposeKif11 shRNA in pMKO.1 retroviral vectorDepositorInsertKif11 shRNA (Kif11 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shAspm
Plasmid#160947PurposeAspm shRNA in pMKO.1 retroviral vectorDepositorInsertAspm shRNA (Aspm Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shCdkn3
Plasmid#160955PurposeCdkn3 shRNA in pMKO.1 retroviral vectorDepositorInsertCdkn3 shRNA (Cdkn3 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shCcnb2
Plasmid#162794PurposeCcnb2 shRNA in pMKO.1 retroviral vectorDepositorInsertCcnb2 shRNA (Ccnb2 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA853 - mec-4p::Fynempty
Plasmid#139210PurposeControl vector for Fyn kinase expression in mec-4 neuronsDepositorInsertFyn (FYN Human)
UseTagsExpressionWormMutationdeletion and frame shift to prevent Fyn expressionPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA850 - osm-10p::FynY531F
Plasmid#139207PurposeExpress activated Fyn kinase in osm-10 neuronsDepositorInsertFyn (FYN Human)
UseTagsExpressionWormMutationY531FPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA851 - osm-10p::Fynempty
Plasmid#139208PurposeControl vector for Fyn kinase expression in osm-10 neuronsDepositorInsertFyn (FYN Human)
UseTagsExpressionWormMutationdeletion and frame shift to prevent Fyn expressionPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUS_LC_withR
Plasmid#139128Purposeexpress FUS LC with the same number and spacing of arginines as hnRNPA2 LCDepositorInsertFUS (FUS Human)
UseTagsExpressionBacterialMutationadd argininesPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_nocharge
Plasmid#139118Purposeexpress hnRNPA2 LC with no charged residuesDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutationall charged residues are changed to S/Q except fo…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_nocharge_wR
Plasmid#139119Purposeexpress hnRNPA2 LC with no charged residues with R added backDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutationremove all K, most DPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUS_LC_charge
Plasmid#139126Purposeexpress FUS LC with an hnRNPA2 LC-like charge patterningDepositorInsertFUS (FUS Human)
UseTagsExpressionBacterialMutationAdd R, K, D to make FUS LC have similar charge pa…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWPXL-p150-PIP1Δ
Plasmid#160993PurposeExpress mouse p150 protein with PIP1 deletion mutationDepositorInsertCHAF1A (Chaf1a Mouse)
UseTagsGFPExpressionMammalianMutationPIP1 (PCNA-interacting protein) motif deletionPromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsNot4_1-274_AG
Plasmid#148750PurposeMammalian Expression of HsNot4_1-274DepositorInsertHsNot4_1-274 (CNOT4 Human)
UseTagsExpressionMammalianMutationone silent mutation compared to the sequence give…PromoterAvailable sinceNov. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGAL4-DBD-OLIG2-Adel-IDR
Plasmid#145249PurposeExpresses fusion of GAL4 DNA-binding domain and OLIG2-Adel IDRDepositorInsertOLIG2-Adel-IDR (OLIG2 Human)
UseTagsExpressionMammalianMutationA-repeats deletedPromoterAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-aC-VTS-US
Plasmid#160425PurposeExpression of c-luciferase from sp. Vargula tsujii (USA)DepositorInsertluciferase
UseLuciferaseTags6x Histidine tag, Yeast alpha secretion factor, c…ExpressionYeastMutationSee depositor comments belowPromoterAOXAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-aC-MSVU-BZ
Plasmid#160426PurposeExpression of c-luciferase from sp. Maristella sp. SVU (Belize)DepositorInsertluciferase
UseLuciferaseTags6x Histidine tag, Yeast alpha secretion factor, c…ExpressionYeastMutationSee depositor comments belowPromoterAOXAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only