Showing: 11201 - 11220 of 14149 results
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Plasmid#170369PurposeNegative control plasmid used for Crispr/cas9 based disruption. Expressing nuclear RFP.DepositorInsertH2B RFP
UseAAVTagsExpressionMutationPromoterhuman SynapsinAvailabilityAcademic Institutions and Nonprofits only -
pAAV.U6gLacZ.hSyn.flex.H2B.RFP
Plasmid#170373PurposeNegative control guide, Cre-inducible plasmid expressing nuclear RFP.DepositorInsertCre inducible H2B RFP
UseAAVTagsExpressionMutationPromoterhuman SynapsinAvailabilityAcademic Institutions and Nonprofits only -
pBEL2196
Plasmid#170400PurposeFor expresson of LetA-[LetB PLL6->1] operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorUseTags8xHis2xQH and TEV cleavage site on LetAExpressionBacterialMutationLetA-LetB(Δ702-717, replaced with 114-130)PromoterAvailabilityAcademic Institutions and Nonprofits only -
pBEL2197
Plasmid#170401PurposeFor expresson of LetA-[LetB PLL6->3] operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorUseTags8xHis2xQH and TEV cleavage site on LetAExpressionBacterialMutationLetA-LetB(Δ702-716, replace with 347-360)PromoterAvailabilityAcademic Institutions and Nonprofits only -
pBEL2198
Plasmid#170402PurposeFor expresson of LetA-[LetB PLL6->5] operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorUseTags8xHis2xQH and TEV cleavage site on LetAExpressionBacterialMutationLetA-LetB(Δ702-716, replace with 580-600)PromoterAvailabilityAcademic Institutions and Nonprofits only -
pBEL2251
Plasmid#170403PurposeFor expresson of LetA-[LetB PLL6->2] operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorUseTags8xHis2xQH and TEV cleavage site on LetAExpressionBacterialMutationLetA-LetB(Δ700-717, replace with 225-249)PromoterAvailabilityAcademic Institutions and Nonprofits only -
pBEL2252
Plasmid#170404PurposeFor expresson of LetA-[LetB PLL6->4] operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorUseTags8xHis2xQH and TEV cleavage site on LetAExpressionBacterialMutationLetA-LetB(Δ700-716, replace with 455-479)PromoterAvailabilityAcademic Institutions and Nonprofits only -
pBEL2253
Plasmid#170405PurposeFor expresson of LetA-[LetB PLL6->7] operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorUseTags8xHis2xQH and TEV cleavage site on LetAExpressionBacterialMutationLetA-LetB(Δ700-717, replace with 812-836)PromoterAvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-CDKL5_IDG-K
Plasmid#170578PurposeGateway destination clone of CDKL5 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-CDKL5 (CDKL5 Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianMutationE854G- please see depositor commentsPromoterUbiquitinAvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_H2B_HaloTag9_T2A_EGFP
Plasmid#169332PurposeNuclear expression of HaloTag9 in mammalian cellsDepositorInsertH2B-HaloTag9 (H2BC21 Human)
UseTagsHaloTagExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_H2B_HaloTag9
Plasmid#169334PurposeNuclear expression of HaloTag9 in mammalian cellsDepositorInsertH2B-HaloTag9 (H2BC21 Human)
UseTagsHaloTag9ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailabilityAcademic Institutions and Nonprofits only -
pYUBcLIC-GFP
Plasmid#183509PurposeThe plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in mycobacteria.DepositorTypeEmpty backboneUseTags10x His-tag and folding reporter GFPExpressionBacterialMutationPromoterT7 lacAvailabilityAcademic Institutions and Nonprofits only -
pETcLIC-GFP
Plasmid#183514PurposeThe plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in E. coliDepositorTypeEmpty backboneUseTags10x His-tag and folding reporter GFPExpressionBacterialMutationPromoterT7 lacAvailabilityAcademic Institutions and Nonprofits only -
pPyCAG-NE-EGFP-IRES-Pac
Plasmid#183617PurposeMammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered EGFP (NLS-EGFP-EmerinTMD). Confers puromycin resistance.DepositorInsertEGFP
UseTagsHuman EMD transmembrane domain and NLSExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-eBFP2-NLS-pA
Plasmid#183800PurposeAAV vector to express nuclear localized eBFP2 from CMV promoterDepositorInserteBFP2-NLS
UseAAVTagsNLSExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAAV-PAG-E2-Crimson-3XNLS-pA
Plasmid#183801PurposeAAV vector to express nuclear localized E2-Crimson from PAG promoterDepositorInsertE2-Crimson-NLS
UseAAVTagsNLSExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAAV-alphaCaMKII-dsRed-Express-3XNLS-pA
Plasmid#183802PurposeAAV vector to express nuclear localized dsRed-Express from CaMKIIa promoterDepositorInsertdsRed-Express
UseAAVTagsNLSExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAAV-alphaCaMKII-E2-Crimson-3XNLS-pA
Plasmid#183803PurposeAAV vector to express nuclear localized E2-Crimson from CaMKIIa promoterDepositorInsertE2-Crimson-NLS
UseAAVTagsNLSExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAAV-Gad65P-eGFP-3XNLS-WPRE-pA
Plasmid#183804PurposeAAV vector to express nuclear localized eGFP from a Gad65 promoterDepositorInserteGFP-NLS
UseAAVTagsNLSExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJCC_103 SpCas9 T1337C
Plasmid#179525PurposeFor bacterial expression of SpCas9 T1337C (for DNA cross-linking) with an N-terminal His-MBP tagDepositorInsertSpCas9 T1337C
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged threonine 1337 to cysteinePromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 11201 - 11220 of 14149 results