Showing: 11181 - 11200 of 14149 results
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Plasmid#174539Purposeencodes AAV-S capsid for efficient gene delivery to the mammalian cochleaDepositorInsertVP1 of AAV9 containing the AAV-S 7-mer peptide insert
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Akt-Scramble
Plasmid#175014PurposeNegative-control construct for Akt-STOPS.DepositorInsertmCherry-Akt-Scramble
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pUC19-whiteCopyCatcher
Plasmid#174062PurposeCopyCatcher insertion donor for the white locusDepositorInsertwhite (w Synthetic, Fly)
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EKAR4
Plasmid#174437PurposeImproved cyan/yellow FRET-based ERK kinase activity reporter.DepositorInsertEKAR4
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-pmEKAR4
Plasmid#174440PurposePlasma membrane-targeted EKAR4.DepositorInsertpmEKAR4
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pBEL2265
Plasmid#175962PurposeFor expresson of LetA-[LetB PLL3->6] operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorInsertsLetA
LetB
UseTags8xHis 2xQH and TEV cleavage site on LetAExpressionBacterialMutationΔ347-361, replace with 702-716PromoteraraBADAvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-tetR-T2A-iRFP
Plasmid#176028Purposeconstruct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome iRFP720DepositorInsertstetR
iRFP
UseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSLQ7334: pHR-PGK-SV40_NLS-dCas12f-VPR-c- Myc_NLS-mCherry-WPRE
Plasmid#176268PurposeExpression of dCas12f-VPR-mCherry in mammalian cellsDepositorInsertdCas12f
UseLentiviralTagsHA-VPR-mCherryExpressionMammalianMutationD326A, D510APromoterPGKAvailabilityAcademic Institutions and Nonprofits only -
pUb PspCas13b + PspCas13b guide RNA
Plasmid#176305PurposeExpresses PspCas13b and its associated guide RNA in Drosophila cellsDepositorInsertPspCas13b
UseTagsHA tagExpressionInsectMutationE113K, N265S- please depositor commentsPromoterUbi-p63eAvailabilityAcademic Institutions and Nonprofits only -
pUb LwaCas13a + LwaCas13a Guide RNA
Plasmid#176307PurposeExpresses LwaCas13a and its associated guide RNA in Drosophila cellsDepositorInsertLwaCas13a
UseTagsHA tagExpressionInsectMutationE656G, I1060V, D1076G- please see depositor comme…PromoterUbi-p63eAvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-tetR-T2A-tSapphire
Plasmid#176312Purposeconstruct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome t-SapphireDepositorInsertstetR
T-sapphire
UseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGFP-Ub-VPS13D
Plasmid#176462Purposeexpressing GFP-Ub marked VPS13D in mammalian cells. GFP-Ub will be cleaved by DUBs to express untagged VPS13DDepositorInsertVPS13D (VPS13D Human)
UseTagsGFP-UbExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pJCC_075 AsCas12a D908A
Plasmid#171668PurposeFor bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tagDepositorInsertAsCas12a D908A
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged aspartate 908 to alaninePromoterAvailabilityAcademic Institutions and Nonprofits only -
pJCC_076 AsCas12a R1226A
Plasmid#171669PurposeFor bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tagDepositorInsertAsCas12a R1226A
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged arginine 1226 to alaninePromoterAvailabilityAcademic Institutions and Nonprofits only -
MCP-ADAR2-DD(E488Q)
Plasmid#170125PurposeLentiviral vector carrying the MCP-ADAR2(E488Q) with a nuclear export signalDepositorInsertMCP-ADAR2-DD(E488Q)-NES
UseLentiviralTagsExpressionMammalianMutationPromoterEf1a core promoterAvailabilityAcademic Institutions and Nonprofits only -
All-in-one split-ADAR2
Plasmid#170136PurposeAAV vector carrying ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN with a nuclear export signalDepositorInsertADAR2-DDC-λN-P2A-MCP-ADAR2-DDN
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
MCP-ADAR2-DD(E488Q, N496F)
Plasmid#170139PurposeAAV vector carrying the MCP-ADAR2-DD(E488Q, N496F) with a nuclear export signalDepositorInsertNES-MCP-ADAR2-DD(E488Q, N496F)
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Split-RESCUE-N
Plasmid#170153PurposeAAV vector carrying the MCP-RESCUE-DDN (RESCUE is the evolved ADAR2 variant for C-U editing) with a nuclear export signalDepositorInsertNES-MCP-RESCUE-DDN
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLV.U6gLacZ.EFS-NS.H2B-RFP
Plasmid#170363PurposeNegative control plasmid used for Crispr/cas9 based disruption. Expressing nuclear RFP.DepositorInsertH2B-RFP
UseLentiviralTagsExpressionMutationPromoterEFS-NSAvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.flex.H2B.GFP
Plasmid#170368PurposeCre-inducible AAV vector expressing nuclear GFP.DepositorInsertflex H2B GFP
UseAAVTagsExpressionMutationPromoterhuman SynapsinAvailabilityAcademic Institutions and Nonprofits only
Showing: 11181 - 11200 of 14149 results