-
Plasmid#49864Purposefor Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 4 copies of the core DSR motif into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTagsyEGFPExpressionMutationPromoterAvailable sinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only
-
pAW8ENdeI-c3HA-8XDSR
Plasmid#49871Purposefor Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 8 copies of the core DSR motif into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTags3XHAExpressionMutationPromoterAvailable sinceDec. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAW8ENdeI-c3HA-4XDSR
Plasmid#49869Purposefor Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 4 copies of the core DSR motif into the S. pombe urg1 locusDepositorTypeEmpty backboneUseCre/LoxTags3XHAExpressionMutationPromoterAvailable sinceDec. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pX330S-2-PITCh
Plasmid#63670PurposeExpresses Cas9 nuclease and the PITCh-gRNADepositorInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRIS-PITChv2-FBL
Plasmid#63672PurposePITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locusDepositorInsertEGFP-2A-PuroR
UseCRISPRTagsExpressionMutationPromoterPromoterlessAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
p201N Cas9
Plasmid#59175PurposeCas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA ShuttleDepositorInsertsCas9
nptII
UseCRISPRTagsExpressionMutationPromoter2x35S and StUbi-3PAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
p201H Cas9
Plasmid#59176PurposeCas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA ShuttleDepositorInsertsCas9
hph
UseCRISPRTagsExpressionMutationPromoter2x35S and StUbi-3PAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX330A_D10A-1x2
Plasmid#58772PurposeExpresses Cas9 nickase and gRNADepositorInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTags3xFLAGExpressionMammalianMutationD10A nickase-converting mutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBBK24 pCAS-Tyr-[gRNA: 7=HIS3] (SplitHygR, AmpR)
Plasmid#179008PurposeSp.Cas9 and gRNA yeast expression vector with HIS3 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
miniTn7(Gm)PA1/04/03-ecfp-a
Plasmid#111619PurposeeCFP Fluorescent tagging of bacteria at their Tn7-siteDepositorInsertPA1/04/03-ecfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330A_dCas9-1x2
Plasmid#63596PurposeExpresses dCas9 and gRNADepositorInserthumanized S. pyogenes dCas9
UseCRISPRTags3xFLAGExpressionMammalianMutationD1361YPromoterCBhAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONRPEX18Km-SceI-pheS
Plasmid#111610PurposeGateway compatible allelic exchange vector for Burkholderia sp.DepositorTypeEmpty backboneUseAllelic exchange in burkholderia sp.TagsExpressionMutationPromoterAvailable sinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
miniTn7(Gm)PA1/04/03-eyfp-a
Plasmid#111620PurposeeYFP Fluorescent tagging of bacteria at their Tn7-siteDepositorInsertPA1/04/03-eyfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAurora
Plasmid#190158PurposeRegulates bacterial expression of target gene in blue-light-activated manner. Based on Nakamurella multipartita PAL.DepositorTypeEmpty backboneUseTagsHis6 tagExpressionBacterialMutationPromoterAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCrepusculo
Plasmid#190156PurposeRegulates bacterial expression of target gene in blue-light-repressed manner. Based on Nakamurella multipartita PAL.DepositorTypeEmpty backboneUseTagsHis6 tagExpressionBacterialMutationPromoterAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330A_D10A-1x7
Plasmid#58777PurposeExpresses Cas9 nickase and gRNADepositorInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTags3xFLAGExpressionMammalianMutationD10A nickase-converting mutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pD864-LacZ
Plasmid#109380PurposeRhamnose inducible LacZ expression. Used to test burden response in E. coliDepositorInsertpRHAM-LacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SCP1-dSa-VPR mini.
Plasmid#99685PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoterDepositorArticleInsertdCas9
UseAAVTagsVPR miniExpressionMutationdead Cas9PromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
Level 1 P4 TaU6 guide acceptor
Plasmid#165600PurposeGoldenGate (MoClo) Level 1 Position 4 TaU6 guide acceptor plasmidInsertTaU6 promoter::LacZ::sgRNA scaffold
UseCRISPR and Synthetic Biology; Sgrna acceptor with…TagsExpressionMutationPromoterAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only