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Plasmid#123930PurposeEncodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator and constitutive NLS::tagBFP as a type 0 part to be used in the MTK systemDepositorInsertPB Destination - Insulator + tagBFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK0_017
Plasmid#123932PurposeEncodes the BxBI attB GFP dropout destination vector constitutive NLS::tagBFP as a type 0 part to be used in the MTK systemDepositorInsertBxBI attB tagBFP destination vector
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
CytERM-mAvicFP1
Plasmid#129510PurposeExpresses CytERM-mAvicFP1 in mammalian cells (for OSER assay)DepositorInsertCytERM-mAvicFP1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10)
Plasmid#86421PurposeN-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter and KAR2 signal sequenceDepositorInsertCDC42pr-KAR2SS-GFP1-10
UseTagsGFP1-10ExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
HSP70l-APEX2-GBP in pDEST-Tol2-pA2-acrys-mCherry
Plasmid#71282PurposeZebrafish expression vector for heat-shock inducible expression of APEX2-GBPDepositorInsertAPEX2-GBP
UseZebrafish expression with tol2TagsExpressionMutationPromoterHSP70lAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-MD2-HA
Plasmid#133065PurposeRetroviral expression of mouse MD2 tagged with HADepositorInsertMD2 (Ly96 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
Sha2Cas9-HF
Plasmid#192142PurposeExpresses highly specific Sha2Cas9, and cloning backbone for sgRNADepositorInsertSha2Cas9-HF
UseAAVTagsExpressionMammalianMutationSha2Cas9-HF (R247A and N415A)PromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
KIF5C(1-560)-2xmCherry-EF(C)
Plasmid#61664PurposeTandem mCherry-labeled, truncated kinesin-1 fused to EF Hand linkerDepositorInsertKIF5C(1-560)-2xmCherry-EF(C) (Kif5c Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
BTK-APEX2-P2A-mKate2
Plasmid#67653PurposeMammalian expression vector driving BTK-APEX2 (Human BTK-PH domain, [marks PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.DepositorInsertBTK-PH-APEX2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
MTK3_001
Plasmid#123952PurposeEncodes nuclear localized PHR domain of CRY2 fused to activating domain of p65 as Type 3 part to be used in the MTK systemDepositorInsert3XNLS::CRY2PHR::p65
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-HA-GFP
Plasmid#120903PurposeExpresses Ptch1-B for function assaysDepositorInsertptch1 (Ptch1 Mouse)
UseTagsGFP and HAExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291PromoterCMVAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
KIF5C(1-560)-GFP11
Plasmid#61670PurposeNon-fluorescent, truncated kinesin-1 fused to split GFP linkerDepositorInsertKIF5C(1-560)-GFP11 (Kif5c Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
MTK0_043
Plasmid#123943PurposeEncodes the piggyBac GFP dropout destination vector with the A1 insulator as a type 0 part to be used in the MTK systemDepositorInsertPB Destination - A1 Insulator - HygroR
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
KIF5C(1-560)-2xmCitrine-GFP11
Plasmid#61667PurposeTandem mCitrine-labeled, truncated kinesin-1 fused to split GFP linkerDepositorInsertKIF5C(1-560)-2xmCitrine-GFP11 (Kif5c Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-JNKsub(TA)DD-YPet-NES
Plasmid#84634PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertJNKsub(TA)DD-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
MTK0_012
Plasmid#123967PurposeEncodes the hROSA26 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK systemDepositorInserthRosa26-BxBI landing pad
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
JPF0522
Plasmid#124013PurposeEncodes crippled pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vectorDepositorInsertPB_pEF1ac-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ERKsub(TA)DD-Venus-NES
Plasmid#84631PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targetedDepositorInsertERKsub(TA)DD-Venus-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationMutated target Thr residue to Ala.PromoterCMVAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
JPF0454
Plasmid#124039PurposeEncodes pPGK1 driving expression of spCas9 P2A mAzamiGreen in a 2nd gen. Lenti Virus destination vectorDepositorInsertpHR_pPGK1-spCAS9-P2A-mAzamiGreen
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK8b_009
Plasmid#123896PurposeEncodes the 3' 500 bp hThal5.10-2-hg18 locus homology arm as a type 8b part to be used in the MTK systemDepositorInsertLA_hThal5.10-2-hg18
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only