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Plasmid#63404PurposeEncodes RVD positions 7-9 in TALEN assemblyDepositorInsertNG NN NN
UseTALENTagsExpressionMutationnonePromoterAvailable sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T Crkl SH2SH3
Plasmid#36404DepositorInsertCrkl SH2 and N-terminal SH3 domains (CRKL Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
enz.043-SETD7-Y305F
Plasmid#154045PurposeExpresses SETD7 (Y305F) in E. coliDepositorInsertHistone-lysine N-methyltransferase SETD7 (SETD7 Human)
UseTagsHis, MBPExpressionBacterialMutationchanged tyrosine 305 to phenylalaninePromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
PA-DAD-dark
Plasmid#53096PurposeExpresses dark mutant PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light with LOV2 in the closed conformationDepositorInsertLOV(dark)-6aa-DAD
UseTags6x His and mVenusExpressionBacterial, Insect, and Mamm…MutationL47F and C49S in Venus; C450M mutation in LOV2PromoterAvailable sinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEE035
Plasmid#176792PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert404
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLuc-Optop38i-dsm
Plasmid#89748PurposeExpression of light-regulated p38 inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor MK3BD3-13FDepositorInsertOptop38i dark-state mutant
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationC450A mutation of AsLov2JalphaPromoterCMVAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-36: AAVS1-mEGFP (PGK)
Plasmid#114404PurposeHomology arms and linker-mEGFP sequence for internal insertion of PGK-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFPDepositorInsertAAVS1 Homology Arms with PGK driven mEGFP (AAVS1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceSept. 4, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pLenti-Lifeact-tdTomato
Plasmid#64048PurposeFluorescent reporter for F-actin labeling in living cellsDepositorInsertLifeact
UseLentiviralTagstdTomatoExpressionMammalianMutationPromoterUbiquitinAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP1-10-miniCMV-GFP11×11-GFP-tDeg
Plasmid#185404PurposeExpresses GFP1-10 and GFP11×11-GFP-tDegDepositorInsertGFP1-10
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDV09_pCMV-ADAR1-p110
Plasmid#200404PurposeExpresses ADAR1 (p110 isoform) constitutively in mammalian cellsDepositorInsertADAR-p110
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_INSR
Plasmid#187772PurposeMAC-tagged gene expression of human INSRDepositorInsertINSR (INSR Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_EPHB4
Plasmid#187768PurposeMAC-tagged gene expression of human EPHB4DepositorInsertEPHB4 (EPHB4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pLminP_Luc2P_RE59
Plasmid#90404PurposeSTAT3 - Sis-inducible element (SIE) gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterIRF1Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
CS2 Flag-Smad2
Plasmid#14042DepositorInsertSmad2 (SMAD2 Human)
UseXenopus/mammalian/avian/zebrafishTagsFlagExpressionMutationPromoterAvailable sinceMay 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_LMTK3
Plasmid#187773PurposeMAC-tagged gene expression of human LMTK3DepositorInsertLMTK3 (LMTK3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYZ033
Plasmid#98404PurposeEntry vector for S. pombe CRISPR-Cas9 system. The gRNA can be integrated easily through Gibson Assembly with the plasmid backbone digested by Not1DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbE5a-Opto-Cre-Vvd
Plasmid#134404PurposeLight-inducible Cre recombinase split with Vivid photodimers for bacterial expression.DepositorInsertOpto-Cre-Vvd
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterlacUV5Available sinceJan. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDFDuet-trAtCCD7-OsD27(pYL726 )
Plasmid#178285PurposeCo-Expresses OsD27 and trAtCCD7 in E. coli. pCDFDuet-1 carrying D27 from Oryza sativa and N-terminus 31 amino-acid truncated CCD7 from ArabidopsisDepositorInsertsOsD27
trAtCCD7
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only