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Plasmid#122016PurposeBaculovirus vector for parallel SLIC cloning containing gp67 signal sequence, N-terminal His6-Smt3Star (SumoStar*) and different C-tag optionsDepositorTypeEmpty backboneUseTagsHis6-Smt3Star and Multi3: depending on the LP2 pr…ExpressionInsectMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only
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pNAS1B split mCherry N-MESVD/TRAP(RK)-C
Plasmid#61967PurposeDuet plasmid for weak split mCherry assembly (as compared to N-ME(pS)VD/TRAP(RK)-C). N-mCherry is fused to the peptide MESVDDepositorInsertsN-mCherry-MESVD
TRAP(RK)-C-mCherry
UseTagsExpressionBacterialMutationPromoterPBAD and PLtetOAvailable sinceFeb. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTlpA-Wasabi-NF
Plasmid#86100PurposeNonfluorescent variant of mWasabi reporter gene gated by wild type TlpADepositorInsertsTlpA
mWasabi
UseTags6xHisExpressionBacterialMutationD341A and S71T, G73APromoterTlpA and TlpA, LacIAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
MS2-ecDHFR
Plasmid#65859PurposeExpresses RNA-binding protein MS2 with eDHFR fusion tagDepositorInsertsUseTagsHA and NLSExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330A_dCas9-1x6
Plasmid#63600PurposeExpresses dCas9 and gRNADepositorInserthumanized S. pyogenes dCas9
UseCRISPRTags3xFLAGExpressionMammalianMutationD1361YPromoterCBhAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
Thy1.2-Roxed-Cre
Plasmid#51276PurposeExpression of the Dre-dependent Roxed-Cre gene under the control of the Thy1.2 expression cassetteDepositorInsertRoxed-Cre
UseTagsExpressionMutationPromoterThy1.2Available sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJD427
Plasmid#154371PurposeExpresses FKBP12-XB and SspBcore-FRB from constitutive promotersDepositorInsertproB-RBS(B0032)-SspBcore-FRB-terminator(B0011)-proC-RBS(B0032)-FKBP12-sspBXB-terminator(B0011)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTlpAreverse-Wasabi
Plasmid#86114PurposemWasabi reporter gene driven by TlpA promoter in reverse orientationDepositorInsertsTlpA
mWasabi
UseTags6xHisExpressionBacterialMutationD341APromoterTlpA (in reverse orientation)Available sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330_HR_Prnp_3
Plasmid#78621PurposepX330 vector encoding SpCas9 and a chimeric guide RNA targeting Prnp coding sequenceDepositorInsertpX330_HR_Prnp_3
UseCRISPR and Mouse TargetingTagsExpressionMammalianMutationPromoterU6Available sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX330A_FokI-1x7
Plasmid#63607PurposeExpresses FokI-dCas9 and gRNADepositorInsertFokI-dCas9
UseCRISPRTagsExpressionMammalianMutationPromoterCBhAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TetR-RNF169UBD
Plasmid#119011PurposeMammalian expression of a fusion protein of single chain Tet repressor with the human RNF169 Ubiquitin binding domain and BFP expressionDepositorInsertTetR
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Pconst-rfp + PmutS-yfp
Plasmid#121444Purposeplasmid reporting the expression from constitutive and mutS promotersDepositorInsertsPmutS-yfp
Pconst-rfp
UseSynthetic Biology; Pbb vectorTagsExpressionBacterialMutationPromoterP-const and P-mutSAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
PacrAB-rfp + Pconst-yfp
Plasmid#121445Purposeplasmid reporting the expression from acrAB and constitutive promotersDepositorInsertsPconst-yfp
PacrAB-rfp
UseSynthetic Biology; Pbb vectorTagsExpressionBacterialMutationPromoterPacrAB and PconstAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYFP-x-1
Plasmid#140142PurposeEvaluating information transmission of a genetic sensor.DepositorInsertlacY (lacY )
UseTagsExpressionBacterialMutationD37, I38, and N39 are changed to nonsense (stop) …PromoterAvailable sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
KG#62
Plasmid#110874PurposeExpresses the C. elegans acy-1 cDNA pan-neuronallyDepositorInsertsrab-3 promoter
acy-1 cDNA
unc-54 3' control region with 1 intron just upstream and 1 intron in control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDHt-KS
Plasmid#63616PurposeThe multiple cloning site of pGreenII0000 cloned into binary vector pDHtDepositorTypeEmpty backboneUseFungal expressionTagsExpressionMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBT374_pattB-pCA-GFP-pA-attBSwa
Plasmid#52553Purposefor PhiC31 integrase-mediated cassette exchangeDepositorInsertsPhiC31o attB (full length)
GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDGE64
Plasmid#79446Purposerecipient plasmid [single nuclease]; pPcUbi:Cas9, nptIIDepositorInsertccdB (ccdB(letD) )
UseCRISPRTagsExpressionPlantMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBACgus_Sumostar/LRRK2 1867-2161_C2024S/C2025S
Plasmid#40394DepositorInsertsUseBaculovirusTags6xHis and HisTEVExpressionMutationaa1867-2161,C2024S/C2025SPromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
EE-SP!gIII
Plasmid#48667PurposeBacterial SP Cas9 targeting filamentous phage gene III at five protospacersDepositorInsertsCas9
anti-gIII tracrRNA
UseCRISPRTagsExpressionMutationPromoterproC and tracrRNA promoterAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only