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Plasmid#102461PurposeExpresses a fusion protein of β2 adrenergic receptor/T4 lysozyme chimera and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.DepositorArticleInsertβ2AR
UseTagsHis tag and sfGFPExpressionBacterialMutationPromoterOR2-OR1-PrAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK371
Plasmid#73596PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-3) with C-terminal c-myc epitopeDepositorInsertsdelta-12 fatty acid acetylenase
delta-12 fatty acid desaturase (form 3)
UseTagsFLAG tag and c-myc tagExpressionYeastMutationPoint mutants in FAD2-3 ORF relative to deposited…PromoterGAL1 and GAL10Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
OA-986B
Plasmid#124999PurposeExpresses dCas9-VPR driven by the Ubiquitin-63E promoterDepositorInsertdCas9-VPR
UsePiggybackTagsExpressionInsectMutationPromoterUbiquitinAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYFP-lacY-1
Plasmid#140138PurposeEvaluating the impact of feedback on information transmission of a genetic sensor.DepositorInsertlacY (lacY )
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcryBFP_Umyo18bGFP
Plasmid#172527PurposealphaA-crystallin promoter (cry) drives GFP expression in the lens. The 600bp unc-45b muscle-specific promoter drives expression of GFP-tagged zebrafish Myo18b.DepositorInsertmyo18b
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBACgus_Sumostar/LRRK2 1867-2186_T2031E
Plasmid#40389DepositorInsertsUseBaculovirusTags6xHis and HisTEVExpressionMutationaa1867-2186, T2031EPromoterpolHAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJFRC7-RFPnls-PQRv2-mCD8:GFP
Plasmid#73974PurposeProtein Quantitation Reporter (PQR) to quantify a protein of interest in insect cells.DepositorInsertssfGFP
RFP
UseTagsmCD8 and nlsExpressionInsectMutationPromoter20XUAS-HSAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
C204
Plasmid#174358PurposeFor expressing siRNA-resistant human PABPC1 in human cell lines via transfectionDepositorInsertPABPC1 (PABPC1 Human)
UseTagsFLAGExpressionMammalianMutationMultiple silent mutations introduced to be resist…PromoterAvailable sinceFeb. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDGE62
Plasmid#79444Purposerecipient plasmid [single nuclease]; p35S:Cas9, nptIIDepositorInsertccdB (ccdB(letD) )
UseCRISPRTagsExpressionPlantMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
OA-1050S (UASt-dCasRx)
Plasmid#132419PurposeExpress dCasRx under UASt promoterDepositorInsertsCasRx
UASt+hsp70
UseTagsExpressionInsectMutationPromoterUAStAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
5n1 (560)
Plasmid#70730PurposeTetR/PhlF/SrpR/LambdaCI/LacI BCD7 5-node v41-2DepositorInsertssrpR-ssrA
lambdaCI-ssrA
phlF-ssrA
lacI-ssrA
tetR-ssrA
UseTagsExpressionMutationPromoterAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXCX-sGFAP-LMO-IRES-tdTomato
Plasmid#178312PurposeExpress the bacterial lactate 2-monooxygenase (LMO) enzyme in astrocytesDepositorInsertLactate 2-monooxygenase
UseAdenoviralTagstdTomatoExpressionMammalianMutationPromotersGFAPAvailable sinceJan. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
MS2_SC2_S_Alpha
Plasmid#175045PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Alpha SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationAlpha (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
MS2_SC2_S_Beta
Plasmid#175046PurposeGeneration of MS2 Virus-Like Particles packaged with the sequence for the WHO Beta SARS-CoV-2 S gene (Amino Acid 319-869)DepositorInsertsUseTagsExpressionBacterialMutationBeta (Amino Acid 319-869)PromoterT7Available sinceOct. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
EC2_1_dCas9_sgRNA
Plasmid#163710PurposeYeast low copy plasmid with dCas9 and sgRNA expression cassetteDepositorInsertdCas9
UseTagsExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ (M)-HT-Cbx7-ATLm
Plasmid#82522PurposeLentivirus vector. Expresses mutant Halotag-Cbx7-ATLm fusion proteins in mammalian cells. Cbx7 ATL mutation of RKR70-72, K75, and KR77-78 to AGA70-72, A75, and AG77-78.DepositorInsertChromobox Homolog 7 (CBX7 Human)
UseLentiviralTagsHaloTagExpressionMammalianMutationmutation of RKR70-72, K75, and KR77-78 to AGA70-7…PromoterCMVAvailable sinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCoofy65
Plasmid#125176PurposeMammalian vector for parallel SLIC cloning containing N-terminal His6-Smt3Star (SumoStar*) and different C-tag optionsDepositorTypeEmpty backboneUseTagsHis6-Smt3Star and Multi3: depending on the LP2 pr…ExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-p19-T7-EGFPFL
Plasmid#46310DepositorInsertsp19
EGFPFL DNA-1
linker
EGFPFL DNA-2
UsePro-sirnaTags6XHis and GSTExpressionBacterialMutationPromoterAvailable sinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
nonphosphorylatable Cenp-B-targted Aurora B FRET sensor
Plasmid#45230DepositorInsertnonphos Cenp-B targeted Aurora B FRET sensor
UseTagsExpressionMammalianMutationt-a mutation in substrate sequencePromoterAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
phpRL-BCL2-FL-pA
Plasmid#42595DepositorInserthairpin - renilla luciferase - BCL2 5'UTR - firefly luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only