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Plasmid#212150PurposePlasmid expressing alanine dehydrogenase under inducible promoter PT7 control. The enzyme is thermophilic and works best at 50 C.DepositorInsertalanine dehydrogenase
UseTags6x HisExpressionBacterialMutationPromoterPT7Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-ODN
Plasmid#208642PurposeEmpty vector for long single strand DNA (up to 1.5k) preparation. This plasmid is a pUC19 with 6 point mutations that remove BspQI, BtsI, BsrDI sites. Mutations adopted from Patent WO/2018/015995.DepositorTypeEmpty backboneUseLong single strand dna productionTagsExpressionMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV MP-135 mRFP-3MREs (AAV-alpha)
Plasmid#206929PurposeAlpha cell-specific gene expression from AAV in human pancreatic isletsDepositorInsertmRFP
UseAAVTagsExpressionMammalianMutationPromoterMP-135Available sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-YES1-6stop
Plasmid#203476PurposeExpresses human YES1 kinase from a GAL10 promoter with a URA3 markerDepositorInsertYES1 (YES1 Human)
UseTagsExpressionYeastMutationPromoterGAL10Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-Bat-SL-CoVZC45_PLP
Plasmid#201939PurposeExpresses Bat-SL-CoVZC45 papain-like protease from a GAL promoter with a URA3 markerDepositorInsertBat-SL-CoVZC45 PLP
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-HCV_NS3-GS-NS4A
Plasmid#203504PurposeExpresses HCV NS3-NS4A protease (with GS linker) from a GAL promoter with a URA3 markerDepositorInsertHCV NS3-GS-NS4A
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-HRV-B14_3C
Plasmid#203466PurposeExpresses HRV-B14 3C protease from a GAL10 promoter with a URA3 markerDepositorInsertHRV-B14 3C protease (HRVBgp1 )
UseTagsExpressionYeastMutationPromoterGAL10Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-DUGV_OTU
Plasmid#203463PurposeExpresses DUGV OTU domain of L protein from a GAL10 promoter with a URA3 markerDepositorInsertDUGV OTU domain of L protein (DUGVsLgp1 )
UseTagsExpressionYeastMutationPromoterGAL10Available sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-HCoV-NL63_PLP2
Plasmid#203502PurposeExpresses HCoV-NL63 PLP2 from a GAL promoter with a URA3 markerDepositorInsertHCoV-NL63 PLP2 (ORF1ab )
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-deltaUBA-short-UBASH3A-Ctag
Plasmid#195397PurposeHuman short-form UBASH3A lacking the UBA domain in Gateway pDONR221 (for C-terminal tag)DepositorInsertUBASH3A (UBASH3A Human)
UseGateway donor vectorTagsExpressionMutationUBASH3A mutant lacking the UBA domainPromoterAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
NXPH1_22-271_AviN_HisC
Plasmid#195872PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertNXPH1 (NXPH1 Human)
UsePfhmsp-avin-lic-c, baculovirus expressionTagsExpressionMutationPromoterPolyhedrinAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmeEF1A_R
Plasmid#147347PurposeInsect Expression of DmeIF1ADepositorInsertDmeIF1A (eIF-1A Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-deltaUBA-short-UBASH3A-Ntag
Plasmid#195398PurposeHuman short-form UBASH3A lacking the UBA domain in Gateway pDONR221 (for N-terminal tag)DepositorInsertUBASH3A (UBASH3A Human)
UseGateway donor vectorTagsExpressionMutationUBASH3A mutant lacking the UBA domainPromoterAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
deltaUBA-short-UBASH3A-V5-6xHis
Plasmid#195404PurposeMammalian expression of human short-form UBASH3A lacking the UBA domain with V5-6xHis tagDepositorInsertUBASH3A (UBASH3A Human)
UseTagsV5-6xHisExpressionMammalianMutationUBASH3A mutant lacking the UBA domainPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGB-05-08-Vps15
Plasmid#187990PurposePlasmid for the protein expression of Vps15 in a bacterial and insect expression system. Internal_Reference: SMC1324DepositorInsertVps15 (PIK3R4 Human)
UseTagsExpressionBacterial and InsectMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
hIgG1-F1-Fc
Plasmid#194597PurposeMammalian expression of F1 nanobody upstream of human IgG1; F1 nanobody targets the receptor binding domain (RBD) of SARS-CoV-2 spikeDepositorInsertF1-Fc (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
hIgG1-E2-Fc
Plasmid#194595PurposeMammalian expression of E2 nanobody upstream of human IgG1; E2 nanobody targets the receptor binding domain (RBD) of SARS-CoV-2 spikeDepositorInsertE2-Fc (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
hIgG1-G6-Fc
Plasmid#194594PurposeMammalian expression of G6 nanobody upstream of human IgG1; G6 nanobody targets the receptor binding domain (RBD) of SARS-CoV-2 spikeDepositorInsertG6-Fc (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
hIgG1-C7-Fc
Plasmid#194596PurposeMammalian expression of C7 nanobody upstream of human IgG1; C7 nanobody targets the receptor binding domain (RBD) of SARS-CoV-2 spikeDepositorInsertC7-Fc (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEXP1-DEST-Noa1-HIS
Plasmid#191972PurposeDestination vector containing HIS-tagged NOA1 coding sequence.DepositorInsertNoa1-HIS (NOA1 Human)
UseSynthetic BiologyTagsHisExpressionMutationPromoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only