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TFORF2546
Plasmid#144209PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCEBPA (CEBPA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-XRCC6
Plasmid#192302PurposeGateway entry vector for an inducible 3xFLAG-XRCC6DepositorInsertXRCC6 (XRCC6 Human)
UseGateway entry vectorTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBbA5a-sufABCDSE
Plasmid#195056PurposeExpresses SUF operon from E. coli from IPTG-inducible promoterDepositorInsertsufABCDSE
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3Luc-5XNF-kappaB
Plasmid#185695PurposeReporter plasmid with a double-strand oligonucleotides that contain five repeats of NF-kappaB binding consensus sequence (5'-GGGGAATTTCC-3').DepositorInsertNF-kappaB (NFKB1 Human)
UseLuciferaseTagsExpressionMutationPromoterNF-kappaB binding siteAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
LgBit-NbALFA
Plasmid#201484PurposeNanoBit detection of ALFA-tagged proteinDepositorInsertLgBit-NbALFA
UseTagsLgBitExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6-sp-sgRNA-RNF2_+41nick
Plasmid#135958PurposeS. pyogenes sgRNA for +41 nick in RNF2DepositorInsertRNF2_+41 nicking sgRNA
UseTagsExpressionMammalianMutationSee manuscriptPromoterU6Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMIEG3-JunDN
Plasmid#40350DepositorInsertJun dominant negative (JUN Human)
UseRetroviralTagsHA and IRES eGFPExpressionMammalianMutationfirst 122 amino acids deletedPromoterAvailable sinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNCS tdTomato
Plasmid#91767PurposeBacterial expression of a fluorescent protein, tandem dimer Tomato (tdTomato)DepositorInserttandem dimer Tomato
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationPromoterT7Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
6His-MBP-TEV-huAsCpf1
Plasmid#90095PurposeBacterial expression plasmid for protein purificationDepositorInserthuAsCpf1
UseTags3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…ExpressionBacterialMutationPromoterAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-UBR5 ∆HECT
Plasmid#52051PurposeCMV driven mammalian expression of ligase-dead UBR5 with an N-termianl GFP fusionDepositorInsertUBR5 ∆HECT (UBR5 Human)
UseTagsGFPExpressionMammalianMutationC2769APromoterCMVAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
JM110
Bacterial Strain#49763PurposeE.coli K-12 derivativeDepositorBacterial ResistanceStreptomycinAvailable sinceOct. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCIP-Flag-TfebAA
Plasmid#79014PurposeFor mammalian expression of a Flag-tagged, constitutively active version (S142A, S211A) of Tfeb.DepositorInsertTfeb (Tfeb Mouse)
UseTagsFlagExpressionMammalianMutationS142A, S211APromoterpCAGGsAvailable sinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP18
Plasmid#22572DepositorInsertUSP18 (USP18 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET23a-H6-TEV-cSrc
Plasmid#214233PurposeBacterial expression of the kinase domain of cSrc with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screeningsDepositorInsertcSrc Kinase domain (SRC Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pICSL01009::AtU6p
Plasmid#46968PurposeEncodes the Arabidopsis U6 promoter in a level 0 vector (Weber et al. PLoS One 6:e16765, 2011). It is used to place an sgRNA uder the Arabidopsis U6 promoter.DepositorInsertAtU6p
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTXB1.Tat(48-57)-Cre recombinase
Plasmid#165042PurposeExpression of Tat(48-57) conjugated Cre recombinase for protein purification (Intein-chitin binding domain fussion)DepositorInsertCre recombinase
UseTagsMxe Intein-Chitin binding domain and Tat(48-57)ExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AAV5cap
Plasmid#198015PurposeFor high-level expression of the AAV5 cap gene, suitable for use with pCMV-Rep78/68 SSM libraryDepositorInsertAAV5 cap
UseAAVTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
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ptf-Galectin3
Plasmid#64149PurposeExpression in mammalian cellsDepositorInsertGalectin3 (LGALS3 Human)
UseTagsEGFP and mRFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only
