Addgene

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pAG665 MAP4-pFAST

Plasmid

#172865

Purpose

Expresses MAP4-pFAST in mammalian cells (microtubule labeling)

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

MAP4-pFAST

Use

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pVRa03_1198

Plasmid

#49655

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF03_1198

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-mEos3.1 (mEosFP3.1-A69T)

Plasmid

#99221

Purpose

Bacterial expression of the primed conversion capable protein pr-mEos3.1 (mEos3.1-A69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos3.1

Use

Tags

Expression

Bacterial

Mutation

A69T for primed conversion applications

Promoter

T7

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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Semaphorin7A-CD4d3+4-bio

Plasmid

#73115

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Semaphorin7A with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Semaphorin 7A (SEMA7A Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG HDAC5 S259A/S498A

Plasmid

#32216

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

HDAC5 (HDAC5 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Ser259Ala, Ser498Ala

Promoter

CMV

Available since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-RNF185-GFP

Plasmid

#113498

Purpose

Expression of human RNF185 with C-terminal GFP tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

RNF185 (RNF185 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-miR30_shBrd9_1061-PGK-NeoR-IRES-mCherry

Plasmid

#75132

Purpose

Retroviral expression plasmid encoding shBrd9_1061 (targeting murine Brd9)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBrd9_1061

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
pMSCVhyg-H3.3K4M-FLAG/HA

Plasmid

#128741

Purpose

Expression of histone H3.3K4M-FLAG/HA

Depositor

Kai Ge

Article

Jang et al Nucleic Acids Res. 2019 Jan 25;47(2):607

Insert

H3.3K4M (H3-3A Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mutation

K4M

Promoter

MSCV

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2+/C-Halo

Plasmid

#184418

Purpose

vector backbone to clone insert downstream of Halo tag (Halo tag codon optimized for Xenopus), for mRNA synthesis

Depositor

Ann Miller

Article

Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do

Type

Empty backbone

Use

For mrna synthesis

Tags

Halo-tag

Expression

Mutation

Codon optimized for expression in Xenopus laevis …

Promoter

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-MAPTau-N-10

Plasmid

#55312

Purpose

Localization: Microtubules, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

MAPTau (MAPT Human)

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pJEP300-pAAV-CMV-MCS2-pA

Plasmid

#112138

Purpose

CMV driven Multi Cloning Site-2

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

N/A

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pTA231-p.Syn-PAmCherry1Hfx

Plasmid

#164661

Purpose

expression of H. volcanii codon-optimized PAmCherry1

Depositor

Anita Marchfelder

Article

Turkowyd et al Front Microbiol. 2020 Nov 20;11:583010.

Insert

PAmCherry1Hfx

Use

Archaeal expression

Tags

Expression

Mutation

codon-optimization for Haloferax volcanii

Promoter

p.Syn

Available since

Feb. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pGEXtev-HMG54kd

Plasmid

#26030

Depositor

Johann Deisenhofer

Article

Istvan et al EMBO J. 2000 Mar 1. 19(5):819-30.

Insert

3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Catalytic domain (amino acids 426-488) Methion…

Promoter

Available since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only

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PTPN13_PDZ_4

Plasmid

#103922

Purpose

Protein expression and purification of PTN13 PDZ4 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PTPN13 (PTPN13 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV5-rat ERK2-K52R

Plasmid

#40813

Depositor

Natalie Ahn

Article

Emrick et al Proc Natl Acad Sci U S A. 2006 Nov 28;10

Insert

MAPK1 (Mapk1 Rat)

Use

Tags

His6

Expression

Mammalian

Mutation

K52R

Promoter

CMV

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-PTK7DN-VSV

Plasmid

#65251

Purpose

Expression of VSV tagged dominant negative PTK7 in mammalian cells

Depositor

Axel Ullrich

Article

Gartner et al PLoS One. 2014 Jan 7;9(1):e84472. doi: 1

Insert

PTK7DN (PTK7 Human)

Use

Tags

VSV

Expression

Mammalian

Mutation

deleted AA 737-1071

Promoter

CMV

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only
pCDFNKI-strepII-3C-LIC-strep

Plasmid

#108708

Purpose

Expression of a StrepII-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Use

Tags

StrepII-3C protease cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-hCas9-T2A-miRFP670

Plasmid

#187223

Purpose

Expresses human Cas9 nuclease and miRFP670 via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

Cas9

Use

Tags

Inserted T2A-miRFP670 at 3' terminal of MCS.

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pRQ-hRasV12

Plasmid

#18113

Depositor

Alex Swarbrick

Article

Swarbrick et al Proc Natl Acad Sci U S A. 2008 Apr 8. 10

Insert

h-RasV12G (HRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

G12V, constitutively active

Promoter

Available since

Feb. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-KIF20A

Plasmid

#192297

Purpose

Gateway entry vector for an inducible 3xFLAG-KIF20A

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

KIF20A (KIF20A Human)

Use

Gateway entry vector

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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