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Plasmid#105397PurposeSynthetic biologyDepositorInsertmYFP (GFP variant) fused to PKI nuclear export signal
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJOG143
Plasmid#105398PurposeSynthetic biologyDepositorInsertmYFP (GFP variant) fused to SV40 nuclear localization signal
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ121
Plasmid#99325PurposeExpress two TALE-VP64 activator linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequenceDepositorInsertTALE-VP64-T2A-TALE-VP64
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprtR161Q
Plasmid#92413PurposehQPRTase harboring R161Q mutationDepositorInserthQPRTase (QPRT Human)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationR161QPromoterT7Available sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEHIAhqprtR161G
Plasmid#92412PurposehQPRTase harbouring R161G mutationDepositorInserthQPRTase (QPRT Human)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationR161GPromoterT7Available sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprtR102Q
Plasmid#92407PurposehQPRTase harboring R102Q mutationDepositorInserthQPRTase (QPRT Human)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationR102QPromoterT7Available sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprtK171S
Plasmid#92404PurposehQPRTase harbouring K171S mutationDepositorInserthQPRTase (QPRT Human)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationK171SPromoterT7Available sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprtR138Q
Plasmid#92411PurposehQPRTase harbouring R138Q mutationDepositorInserthQPRTase (QPRT Human)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationR138QPromoterT7Available sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprtR102A
Plasmid#92405PurposehQPRTASE harbouring R102A mutationDepositorInserthQPRTase (QPRT Human)
UseTagsTEV protease cleavable 6xHisExpressionBacterialMutationR102APromoterT7Available sinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_38J
Plasmid#91154PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: nos:npt IIDepositorInsertEngineering Reagent: 35S:TALEN
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_39J
Plasmid#91155PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: nos:barDepositorInsertEngineering Reagent: 35S:TALEN
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
TF101-STTM-miR394
Plasmid#84813PurposeTurning off/down miR394 in SoybeanDepositorInsertSTTM-miR394
UseTagsExpressionPlantMutationbp8 is deleted- please see depositor comments bel…PromoterunknownAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - MPX
Plasmid#89619Purpose[Edit] Expresses recombinant 6HIS tagged T. cruzi mitochondrial tryparedoxin peroxidase protein in E. coli upon IPTG inductionDepositorInsertMitocondrial Tryparedoxin peroxidase
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - EtfA
Plasmid#89616PurposeExpresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein A in E. coli upon IPTG inductionDepositorInsertElectron transport flavoprotein alpha subunit
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - EtfB
Plasmid#89617PurposeExpresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein B in E. coli upon IPTG inductionDepositorInsertelectron-transfer-flavoprotein, alpha polypeptide
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - TDR
Plasmid#89627PurposeExpresses recombinant 6HIS tagged T. cruzi thiol dependent reductase in E. coli upon IPTG inductionDepositorInsertthiol dependent reductase
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceApril 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - NFDD
Plasmid#89624PurposeExpresses recombinant 6HIS tagged T. cruzi NAD-FAD–dependent dehydrogenase in E. coli upon IPTG inductionDepositorInsertNAD/FAD dependent dehydrogenase
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceApril 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-6NBC-GFP-PGK-Puro-Barcode9
Plasmid#83686Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode9
UseRetroviralTagsExpressionMutationplease view depositor comments belowPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
FCIV1-GR SS287AA
Plasmid#72703PurposeLentiviral expression of GR Phospho-deficient mutant (2nd generation)DepositorInsertGR (Nr3c1 Rat)
UseLentiviralTagsIRES-VenusExpressionMammalianMutationSS287AAPromoterubiquitinAvailable sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHL671
Plasmid#62825PurposeLim Lab plasmid: AmpR + PCon::glgC::gfp::T1T2 terminator + ColE1DepositorInsertgfp
UseSynthetic BiologyTagsglgC leaderExpressionBacterialMutationPromoterpConAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only