Addgene

We narrowed to 16,584 results for: multi

Showing: 11041 - 11060 of 16584 results

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pTK101

Plasmid

#59561

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) mKate-G8-L7Ae-PEST 4xFF4

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) mKate-G8-L7Ae-PEST 4xFF4

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-IndHom-Tol2-FRT-GalK-cryaa-dsRed

Plasmid

#73203

Purpose

BAC targeting cassette for pIndigoBAC-536 with GalK selection marker and lens-specific transgenesis marker dsRed

Depositor

Holger Knaut

Article

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

dsRed

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only

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LacI INCENP

Plasmid

#45236

Purpose

Target INCENP to lac operator sequence

Depositor

Michael Lampson

Article

Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

Insert

LacI, INCENP-Δcen, mCherry (lacI )

Use

Tags

LacI (lac repressor protein) and mCherry

Expression

Mammalian

Mutation

CEN box deleted from INCENP (aa1-46)

Promoter

t7

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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PP363

Plasmid

#78984

Purpose

255B. Promoter: scTEF1, minimal Promoter: GAP. Figure 5. Expresses rHGH and IFNa2b.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Inserts

rHGH
IFNa2b

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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p15aC-4D1D

Plasmid

#107864

Purpose

Bacterial expression of PIS and PI4K

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Inserts

PIS
phosphatidylinositol 4-kinase β

Use

Tags

myc

Expression

Bacterial

Mutation

Promoter

proD

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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XW54

Plasmid

#65839

Purpose

Punc-4c-NLS-QF-BD-DM-Nzipper-SL2-mCherry-3'UTR-unc-54 for Split Q intersectional method

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

NLS-QF-BD-DM-Zipper

Use

Tags

mCherry

Expression

Worm

Mutation

Promoter

Punc-4c

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pVD_85

Plasmid

#149604

Purpose

(parent plasmid pCFB257) pX - 3 -LoxP -KlLEU2 -pTEF1 - XylR

Depositor

Michael Jensen

Article

Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

Insert

XylR

Use

Synthetic Biology

Tags

SV40

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pX330A_dCas9-1x4

Plasmid

#63598

Purpose

Expresses dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

humanized S. pyogenes dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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px459 EQR

Plasmid

#101732

Purpose

Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 EQR

Use

Tags

Expression

Mammalian

Mutation

VQR

Promoter

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pPJ167(JPUB_006765)

Plasmid

#83831

Purpose

Carries NEW operon 3 final

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Inserts

Kuste2803
Kuste2804

Use

Tags

Expression

Mutation

Promoter

op3_2804_for and op3_BsmBI_2803_for

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-TarHom-Tol2-FRT-GalK-cryaa-CFP

Plasmid

#73200

Purpose

BAC targeting cassette for pTARBAC2 with GalK selection marker and lens-specific transgenesis marker Cerulean

Depositor

Holger Knaut

Article

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

CFP

Use

Tags

Expression

Mutation

Promoter

Available since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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pRVdG-EGFP-N-P-M-nucBFP-SynPhRFP-L

Plasmid

#52478

Purpose

Expresses EGFP, nuclear mTagBFP, and Synaptophysin-TagRFP-T

Depositor

Sebastian Seung

Article

Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

Inserts

EGFP
nuclear mTagBFP
Synaptophysin-TagRFP-T

Use

Rabies virus

Tags

TagRFP-T

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pML2003

Plasmid

#193255

Purpose

Propionate sensor expressing eYFP reporter

Depositor

Lauren Andrews

Article

Lebovich et al Front Bioeng Biotechnol. 2022 Aug 25;10:

Inserts

prpR
eYFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Pprp and Synthetic promoter P6

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLN592 (FuGW-S(USF1)p-mKate2)

Plasmid

#105204

Purpose

synthetic promoter USF1 drives mKate2 expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(USF1)p-mKate2

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pBT316_pattB-pCA-GFP-pA

Plasmid

#52548

Purpose

for Phic31 integrase-mediated transgene integration

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pDB026g

Plasmid

#109263

Purpose

Accessory plasmid for PACE of MjTyrRS variants

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Ppsp_pIII(P29*, P83*, T177*, Y184*); luxAB; PproK_TyrT(Opt, CUA);

Use

Tags

Expression

Bacterial

Mutation

amber codon at position 29, 83, 177, and 184 of g…

Promoter

Ppsp and PproK

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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Id2-Halo donor

Plasmid

#113116

Purpose

Donor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Id2 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Id2 donor region including homology arms and HaloTag

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoClover-SpHis5

Plasmid

#44857

Purpose

Yeast tagging vector to create gene fusion with yeast optimized Clover with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoClover

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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B108-GFP-IRES-AP

Plasmid

#73988

Purpose

B108 enhancer of Blimp1 gene drives EGFP expression.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

B108 enhancer

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-TetR-Rad18UBD

Plasmid

#119010

Purpose

Mammalian expression of a fusion protein of single chain Tet repressor with the human Rad18 Ubiquitin binding domain and BFP expression

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

TetR

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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