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Plasmid#184724PurposeEP2-KO in MDCKDepositorInsertA gRNA targeting the dog EP2 gene.
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-bleo-PLA2G4A_3
Plasmid#184725PurposecPLA2-KO in MDCKDepositorInsertA gRNA targeting the dog cPLA2 gene.
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-hyg2-PTGER4
Plasmid#184726PurposeEP4-KO in MDCKDepositorInsertA gRNA targeting the dog EP4 gene.
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX459-canis_PTGS1-3
Plasmid#184727PurposeCOX1-KO in MDCKDepositorInsertA gRNA targeting the dog COX1 gene.
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX459-canis_PTGS1-4
Plasmid#184728PurposeCOX1-KO in MDCKDepositorInsertA gRNA targeting the dog COX1 gene.
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX459-canis_PTGS2-3
Plasmid#184729PurposeCOX2-KO in MDCKDepositorInsertA gRNA targeting the dog COX2 gene.
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-4493NES(Booster-PKA)
Plasmid#184710PurposePKA biosensorDepositorInsertBooster-PKA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-UbC-4493NES(Booster-PKA)
Plasmid#184711PurposePKA biosensorDepositorInsertBooster-PKA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCSIIhyg-iRFP670-P2Av3-RGECO1.0
Plasmid#184713PurposeCalcium biosensorDepositorInsertGCaMP6s
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-pOTC-GFP
Plasmid#205724PurposeExpression of GFP fused with pOTC, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylaseDepositorInsertpOTC-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
6xABRE_R
Plasmid#185804Purposea synthetic ABA signaling reporter designed by multimerization of ABRE (7bp) and its flanking sequences from the RD29A promoterDepositorInsert6xABRE(RD29A)+-86MP+erGFP
UseTagsExpressionPlantMutationPromoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMOSyt1C2AB(80-421)
Plasmid#209005PurposeRecombinant protein expression of truncated syt1 constructDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-SUMOExpressionBacterialMutationaa 80-421 onlyPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-ODN-hsRPL3-CterTwStrep
Plasmid#208644Purposefor long single strand DNA donnor preparation for Twin Strep tag knock-in at C ter of human RPL3. Digest with nt.BspQI and nb.BsrDI to get ssDNA. This plasmid will cause re-edit and fail knock-in.DepositorInsertTwin Strep tag with RPL3 genome HomoArm (RPL3 Synthetic, Human)
UseCRISPR; Ssdna donnor preparationTagsTwin StrepExpressionMutationPromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-msEGFP1.8
Plasmid#208619PurposeMammalian expression of a EGFP variant. Mutations were adopted from (Addgene#135301, PMC7508230), named msEGFP1.8 because N-ter and C-ter features of that in msGFP2 (PMC7508230) not included.DepositorInsertmsEGFP1.8
UseTagsExpressionMammalianMutation(compared with pEGFP-C1, protein level) S30R, Y39…PromoterCMVAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCALPS-EGFP-Cre
Plasmid#207132PurposeLentiviral vector to express EGFP-CreDepositorInsertEGFP-Cre
UseLentiviralTagsExpressionMutationPromoterSSFVAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZa pLtetO CsgA-SpyTag
Plasmid#204503PurposeBacterial expression of CsgA with C-terminal SpyTag peptide and GS linkerDepositorInsertCsgA
UseSynthetic BiologyTags6xHis, SpyTagExpressionBacterialMutationPromoterpLtetOAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
RPO41 deltaN380
Plasmid#205076PurposeRPO41 protein expressionDepositorInsertRPO41 (RPO41 Budding Yeast)
UseTagsExpressionBacterialMutationLacks N Terminal 380 amino acidsPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
RPO41 deltaN296
Plasmid#205075PurposeRPO41 protein expressionDepositorInsertRPO41 (RPO41 Budding Yeast)
UseTagsExpressionBacterialMutationLacks N Terminal 296 amino acidsPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETSyt1C2AB(80-421)msGFP
Plasmid#209009PurposeRecombinant protein expression of truncated syt1 tagged with GFPDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis and msfGFPExpressionBacterialMutationaa 80-421 onlyPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETSUMOSyt1C2AB(96-421)
Plasmid#209006PurposeRecombinant protein expression of truncated syt1 constructDepositorInsertsyt1 (Syt1 Rat)
UseTags6xHis-SUMOExpressionBacterialMutationaa 96-421 onlyPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only