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Plasmid#222421PurposeTo express a label of ER-plasma membrane contacts based on GFP-MAPPER (#117721), but with mScarletI3 tag and replacing FRB with a protein of similar structure and sequence length (Neoleukin-2/15)DepositorInsertMAPPER
UseTagsmScarletI3-6DG5ExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMX-Gal4-TEAD4
Plasmid#33105DepositorInsertTEAD4 (TEAD4 Human)
UseTagsGal4ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFLAG UBR1 SBX
Plasmid#24506DepositorInsertUBR1 (UBR1 Budding Yeast)
UseTagsFLAGExpressionYeastMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Merlin
Plasmid#84293PurposeExpression of wt MerlinDepositorInsertMerlin (NF2) (NF2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2-Opti
Plasmid#163126PurposeLentiCRISPRv2 variant with modified sgRNA scaffold from Chen et al. 2013 Cell.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLGB29
Plasmid#126619PurposeSuicide vector for allelic replacement in Bacteroides, inulin selection and aTC-inducible ss-Bfe1 counterselectionDepositorTypeEmpty backboneUseAllellic exchange in bacteriaTagsExpressionMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
ER-mRFP
Plasmid#62236PurposePlasmid expresses endoplasmic reticulum targeted mRFP in mammalian cells.DepositorInsertER-mRFP
UseTagsmRFP1ExpressionMammalianMutationPromotercmvAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CRISPR-Puro
Plasmid#208646PurposepcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.DepositorTypeEmpty backboneUseCRISPRTagsClawed frog nucleoplasmin NLS, Flag, Puromycin re…ExpressionMammalianMutationPromoterCMV and U6 in tandemAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-GFP1-10
Plasmid#78591PurposeExpress mCherry-GFP1-10 in mammalian cellsDepositorInsertGFP 1-10
UseTagsmCherry2ExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
p1321 HPV-16 E6/E7
Plasmid#8641DepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
XOR gate-V2.0
Plasmid#44453DepositorInsertXOR-gate
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
XNOR-gate-V2.0
Plasmid#44454DepositorInsertXNOR-gate
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCL20C-ddIPpoI
Plasmid#49053PurposeExpresses the restriction enzyme IPpoI under the control of Shield1DepositorInsertDD-HA-ER-IPpoI
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti-puro-LacZ
Plasmid#39477DepositorInsertLacZ
UseLentiviralTags6xHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJYDNp
Plasmid#162451PurposePositive control plasmid carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid serves as an expression control.DepositorInsertAga2p-eUnaG2
UseShuttle vector bacteria/yeastTagsHA and mycExpressionMutationPromoterGAL1Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-N-WASP
Plasmid#47406DepositorInsertN-WASP
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-sfGFP
Plasmid#199171Purposeused to express a superfolder GFP in E. coli for comparison to supercharged variantsDepositorInsertsuperfolder GFP
UseTagsMGHHHHHHGGAExpressionMutationPromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
p5953 pcDNA-Xpress-His-USP15
Plasmid#23216DepositorInsertUSP15 (USP15 Human)
UseTagsHis and XpressExpressionMammalianMutationPromoterAvailable sinceApril 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
PSD95-TS:YFP
Plasmid#42225DepositorInsertPSD95
UseTagsTS:YFPExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pXF87
Plasmid#139027PurposeMosSCI backbone for MosSCI-SapTrap assemblyDepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only