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Addgene
Showing: 10981 - 11000 of 21165 results

  1. pKSB- PUb-DsRedSV40 GGGG AAGA

    Plasmid
    #174538
    Purpose
    PUb-DsRed fluorescence marker cassette for gene knock-in in mosquitoes
    Depositor
    Eric Marois
    Insert
    PUb-DsRed BsaI cassette
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Aedes aegypti Polyubiquitin
    Availability
    Academic Institutions and Nonprofits only
    174538-plasmid-map-sequence-id-343230

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  2. pRDA_221

    Plasmid
    #169142
    Purpose
    Constitutive expression of EGFP and sgRNAs targeted to EGFP for measurement of activity of AsCas12a\EnAsCas12a.
    Depositor
    John Doench, David Root
    Insert
    EGFP , EGFP sgRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    169142-plasmid-map-sequence-id-334957

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  3. pSpCas9(BB)-2A-Hygro

    Plasmid
    #127763
    Purpose
    Used for expression of sgRNA in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    127763-plasmid-map-sequence-id-247560

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  4. XLone-PE2

    Plasmid
    #136463
    Purpose
    Expresses prime editing 2 (PE2) enzyme upon doxycycline treatment
    Depositor
    Xiaojun Lian
    Insert
    PE2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3GS
    Availability
    Academic Institutions and Nonprofits only
    136463-plasmid-map-sequence-id-272540

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  5. pCMV-ABE8e-NRCH

    Plasmid
    #165416
    Purpose
    Expresses the base editor ABE8e-NRCH in mammalian cells
    Depositor
    David Liu
    Insert
    ABE8e-NRCH
    Use
    Tags
    BP-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    165416-plasmid-map-sequence-id-334022

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  6. pRha-ABE8e-NRCH

    Plasmid
    #165417
    Purpose
    For bacterial purification of ABE8e-NRCH protein
    Depositor
    David Liu
    Insert
    ABE8e-NRCH
    Use
    Tags
    8xHIS and BP-NLS
    Expression
    Bacterial
    Mutation
    Promoter
    pRhaBAD
    Availability
    Academic Institutions and Nonprofits only
    165417-plasmid-map-sequence-id-334014

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  7. pHTganA-Cpf1

    Plasmid
    #158647
    Purpose
    Constitutive transcription of FnCpf1 and ganA crRNA
    Depositor
    Wenjing Cui
    Insert
    Cpf1, ganA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P43
    Availability
    Academic Institutions and Nonprofits only
    158647-plasmid-map-sequence-id-314144

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  8. pADsacA

    Plasmid
    #158649
    Purpose
    Constitutive transcription of sacA crRNA and GFP
    Depositor
    Wenjing Cui
    Insert
    sacA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pveg
    Availability
    Academic Institutions and Nonprofits only
    158649-plasmid-map-sequence-id-310569

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  9. pBsacA

    Plasmid
    #158650
    Purpose
    Constitutive transcription of sacA crRNA
    Depositor
    Wenjing Cui
    Insert
    sacA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pveg
    Availability
    Academic Institutions and Nonprofits only
    158650-plasmid-map-sequence-id-314145

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  10. pAAVS1-CAG-hCAS9-neo

    Plasmid
    #166026
    Purpose
    AAVS1 targeting vector carrying CAG-SpCas9-neo
    Depositor
    Kosuke Yusa
    Insert
    AAVS1-CAGCas9IRESneo
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    166026-plasmid-map-sequence-id-328056

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  11. HCP5

    Plasmid
    #166107
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNAs that targets the C-terminus of Cyr1.
    Depositor
    Matthias Heinemann
    Insert
    Cyr1CT-sgRNA (CYR1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    166107-plasmid-map-sequence-id-330320

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  12. pAct-Fok1:dCas9

    Plasmid
    #62211
    Purpose
    Expresses Fok1:dCas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous high specificity genome engineering in Drosophila melanogaster.
    Depositor
    Simon Bullock
    Insert
    Fok1-dCas9
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    act5C
    Availability
    Academic Institutions and Nonprofits only
    62211-plasmid-map-sequence-id-101870

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  13. pCASCADE-gltA2

    Plasmid
    #65817
    Purpose
    gltA targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    gltA2 guide
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    E . coli ugpB gene promoter, low phosphate induc…
    Availability
    Academic Institutions and Nonprofits only
    65817-plasmid-map-sequence-id-111972

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  14. pCASCADE-udhA

    Plasmid
    #65818
    Purpose
    udhA targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    udhA guide
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    E . coli ugpB gene promoter, low phosphate induc…
    Availability
    Academic Institutions and Nonprofits only
    65818-plasmid-map-sequence-id-111976

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  15. pCASCADE-zwf

    Plasmid
    #65825
    Purpose
    zwf targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    zwf guide
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    E . coli ugpB gene promoter, low phosphate induc…
    Availability
    Academic Institutions and Nonprofits only
    65825-plasmid-map-sequence-id-124656

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  16. TLCV2-LoxP

    Plasmid
    #127098
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system.
    Depositor
    Adam Karpf
    Insert
    Cas9-2A-eGFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    127098-plasmid-map-sequence-id-246495

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  17. TLCV2 sgAAVS1

    Plasmid
    #127099
    Purpose
    Encodes gRNA for human AAVS1.
    Depositor
    Adam Karpf
    Insert
    AAVS1 (PPP1R12C AAVS1 Human) (PPP1R12C Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    127099-plasmid-map-sequence-id-246497

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  20. hSLBP_mNG_FS_Donor

    Plasmid
    #191387
    Purpose
    Donor for C-terminal tagging of endogenous hSLBP
    Depositor
    Yannick Doyon
    Insert
    mNeonGreen(mNG) + FS(3xFLAG_TwinStrep)Tag for hSLBP tagging at the C-terminus (SLBP Synthetic, Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    191387-plasmid-map-sequence-id-378113

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Showing: 10981 - 11000 of 21165 results