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pLenti_CMV_Puro_SFB_GNB1L

Plasmid

#224381

Purpose

Lenti plasmid for generating GNB1L expressing stable cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pX459_TELO2_gRNA

Plasmid

#224379

Purpose

Targets TELO2 for dTAG N terminal knock-in cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

TELO2 (TELO2 Human)

Use

CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanII-56

Plasmid

#225073

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-56 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-56)

Use

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-mCRT-GPI-IRES-DsRed

Plasmid

#223711

Purpose

Mammalian expression of mouse CRT-GPI

Depositor

Judy Lieberman

Article

Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

Insert

Calreticulin-GPI (Calr Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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His-PHD2-181-426

Plasmid

#223551

Purpose

His-tagged human PHD2 catalytic domain with TEV cleavage site between His and PHD2 for PHD2 purification

Depositor

Somshuvra Mukhopadhyay

Article

Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

Insert

PHD2 (EGLN1 Human)

Use

Tags

6x His

Expression

Bacterial

Mutation

Deleted amino acids 1-180.

Promoter

trc

Available since

Sept. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-859

Plasmid

#218009

Purpose

mCerulean fluorescent protein fused to Factor Xa Protease cleavage site and Strep-Tag II

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

mCerulean

Use

Synthetic Biology

Tags

Factor Xa protease cleavage site and Strep-tag II

Expression

Mutation

Promoter

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-839

Plasmid

#217995

Purpose

sAP1 protein secretion signal

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

Secretion signal peptide derived from the secretory protein secreted acid phosphatase 1 (sAP1)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-845

Plasmid

#218000

Purpose

Strep-Tag II fused to PreScission Protease cleavage site for protein purification and cleavage

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

Strep-tag II

Use

Synthetic Biology

Tags

PreScission protease cleavage site

Expression

Mutation

Promoter

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-849

Plasmid

#218004

Purpose

8xHis-tag fused to PreScission Protease cleavage site for protein purification and cleavage

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

8xHis-tag

Use

Synthetic Biology

Tags

PreScission protease cleavage site

Expression

Mutation

Promoter

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28b C7H1G6_6H N-terminal

Plasmid

#223434

Purpose

C7H1G6 GNAT expression vector with N-terminal His6 tag

Depositor

Emily Balskus

Article

Mehta et al Nat Med. 2023 Mar;29(3):700-709. doi: 10

Insert

C7H1G6 GNAT

Use

Tags

His6

Expression

Mutation

Promoter

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28b Salmonella NAT_6H C-terminal

Plasmid

#223436

Purpose

Salmonella NAT expression vector with C-terminal His6 tag

Depositor

Emily Balskus

Article

Mehta et al Nat Med. 2023 Mar;29(3):700-709. doi: 10

Insert

Salmonella NAT

Use

Tags

His6

Expression

Mutation

Promoter

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28b R6CZ24_6H C-terminal

Plasmid

#223435

Purpose

R6CZ24 Thiolase expression vector with C-terminal His6 tag

Depositor

Emily Balskus

Article

Mehta et al Nat Med. 2023 Mar;29(3):700-709. doi: 10

Insert

R6CZ24 Thiolase

Use

Tags

His6

Expression

Mutation

Promoter

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-pCAG-donor(R2Tg)-GFP(intron)

Plasmid

#223248

Purpose

Mammalian expression of the R2Tg RNA donor

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg donor

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1_Zeo_TetO_FLAG-AT1R

Plasmid

#222856

Purpose

Mammalian expression plasmid for FLAG-tagged AT1R

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT1R (AGTR1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Blasticidin XLone-eGFP

Plasmid

#159754

Purpose

AAVS1 donor plasmid for targeted inducible eGFP expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Biomaterials. 2022 May 6;285:121569. doi

Insert

all-in-one tet-on system

Use

CRISPR and TALEN; Donor plasmid for targeted knoc…

Tags

Expression

Mammalian

Mutation

Promoter

TRE3GS inducible promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-eGFP_KDEL

Plasmid

#221411

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

eGFP_KDEL

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-eGFP_myr

Plasmid

#221412

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

eGFP_myr

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-eGFP

Plasmid

#221413

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-eGFP_mito

Plasmid

#221414

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

eGFP_mito

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-SLC66A2_STOP

Plasmid

#221419

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC66A2 (PQLC1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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