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Plasmid#177011PurposeGenerate baculovirus for insect cell expression of CDK1 with N-terminal GST-3C (PreScission)DepositorInsertCDK1 (CDK1 Human)
UseTagsGST-3CExpressionInsectMutationcodon-optimised for insect cell expressionPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-GFP(39TAG)
Plasmid#217360PurposeExpression of reporter EGFP with a TAG stop codon at 39 aa position; can be packaged into bacmidDepositorInsertEnhanced green fluorescent protein
UseBaculoviralTagsExpressionInsect and MammalianMutationY39TAGPromoterCMVAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
MultiMate 5 colors
Plasmid#206266PurposeExpression of 5 fluorescently labelled proteins in mammalian cells. Can be used as a CRE-donor (MultiBac system)DepositorInsertH2B (H. Sapiens), Actin and Tubulin (B. taurus), mito mCherry (Synthetic), CyOFP1 (E. quadricolor)
UseRecombinant baculovirus production, cre acceptor…TagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTA-Mob 2.0 Tp
Plasmid#188619PurposeMutated pTA-Mob 2.0 Backbone in TraJ Promoter RegionDepositorTypeEmpty backboneUseSynthetic Biology; Conjugation helper plasmidTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual α1B/β2B Tubulin
Plasmid#170553PurposeEncodes codon-optimized human α1B and β2B tubulin for expression in and purification from insect cells.DepositorUseTagsAP linker, GS linker (GGSGG), His10 tag, L21 enha…ExpressionInsectMutationPromoterAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMB5-PSMB6-PSMB7-TEV-2xSTII
Plasmid#217896PurposeInsect cell expression of human 20S CP proteasome subunit beta type-5 (PSMB5), beta type-6 (PSMB6), and beta type-7 (PSMB7) with C-terminal Twin-Strep-tagDepositorUseTags2xSTIIExpressionInsectMutationPromoterAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMA5-PSMA6-PSMA7
Plasmid#217894PurposeInsect cell expression of human 20S CP proteasome subunit alpha type-5 (PSMA5), alpha type-6 (PSMA6), and alpha type-7 (PSMA7)DepositorUseTagsExpressionInsectMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual α1B/β3 Tubulin
Plasmid#170552PurposeEncodes codon-optimized human α1B and β3 tubulin for expression in and purification from insect cells.DepositorUseTagsAP linker, GS linker (GGSGG), His10 tag, L21 enha…ExpressionInsectMutationPromoterAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-KPNB1
Plasmid#192301PurposeGateway entry vector for an inducible 3xFLAG-KPNB1DepositorInsertKPNB1 (KPNB1 Human)
UseGateway entry vectorTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACE-K2P10.1-StrepTagII
Plasmid#191475PurposeExpresses K2P10.1 with C-terminal StrepTagIIDepositorUseTagsExpressionInsectMutationN144Q (uniprot P57789-1)PromoterpolHAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-KPNA2
Plasmid#192306PurposeGateway entry vector for an inducible 3xFLAG-KPNA2DepositorInsertKPNA2 (KPNA2 Human)
UseGateway entry vectorTags3xFLAGExpressionMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBdest-GST-PI3Kc1
Plasmid#187992PurposePlasmid for the protein expression of GST-3C-ATG14/Vps34/Vps15/BECN1 in a bacterial and insect expression system. Internal_Reference: SMC1326DepositorUseTagsGSTExpressionBacterial and InsectMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas9-beta-mmr
Plasmid#169241PurposeCas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutationsDepositorInsertscas9
beta
mutL-E36K
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationChanged glutamic acid 36 to lysinePromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
SPRR2A-pFastBac I
Plasmid#182460PurposeExpression His-tagged SPRR2A in insect cellsDepositorInsertsmall proline rich protein 2A (SPRR2A Human)
UseTags6xHisExpressionInsectMutationPromoterPolyhedrinAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
ArpC4_pLib
Plasmid#173681PurposeArpC4 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expressionDepositorInsertArpC4 (ARPC4 Human)
UseTagsExpressionInsectMutationPromoterpolyhedrinAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT JunD-HA
Plasmid#58498PurposeGateway entry vector for an inducible HA-tagged mouse JunDDepositorInsertJunD (Jund Mouse)
UseEntry vectorTagsHAExpressionMutationPromoterAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt13C(S473D)
Plasmid#86577PurposeExpresses cleavable human full-length Akt1(S473D) phosphomimeticDepositorInsertAkt1 (AKT1 Human)
UseTags10xHisExpressionInsectMutation3C cleavage site inserted at pos. 130; S473D phos…PromoterAvailable sinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
ArpC5_pLib
Plasmid#173682PurposeArpC5 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expressionDepositorInsertArpC5 (ARPC5 Human)
UseTagsExpressionInsectMutationPromoterpolyhedrinAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
ArpC1_pLib
Plasmid#173678PurposeArpC1 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expressionDepositorInsertArpC1 (ARPC1B Human)
UseTagsExpressionInsectMutationPromoterpolyhedrinAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
His-ZZ-TEV-BICDR1
Plasmid#111859PurposeFull length BICDR1 for expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8x-His-ZZ.DepositorInsertBICDR1 (Bicdl1 Mouse)
UseTagsHis-tag, TEV site, and ZZExpressionInsectMutationPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only