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Addgene
Showing: 1081 - 1100 of 1204 results

  1. pFLIP39

    Plasmid
    #65507
    Purpose
    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Article
    Jones et al Elife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Use
    Tags
    6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMyc
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
    65507-plasmid-map-sequence-id-111416

    Enlarge

  2. pDRFLIP32

    Plasmid
    #65511
    Purpose
    FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Article
    Jones et al Elife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Use
    Tags
    6His-AFPt9-attR1 and attR2-t7CFPt9-cMyc
    Expression
    Yeast
    Mutation
    Promoter
    pPMA1
    Availability
    Academic Institutions and Nonprofits only
    65511-plasmid-map-sequence-id-111421

    Enlarge

  3. pDRFLIP39

    Plasmid
    #65517
    Purpose
    FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Article
    Jones et al Elife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Use
    Tags
    6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMyc
    Expression
    Yeast
    Mutation
    Promoter
    pPMA1
    Availability
    Academic Institutions and Nonprofits only
    65517-plasmid-map-sequence-id-111427

    Enlarge

  9. Stx1a-12aa-mCer

    Plasmid
    #53236
    Purpose
    Fluorescent probe: Syntaxin 1A, C-terminally-tagged with monomeric Cerulean, connected with a 12 amino acid linker. Expressed in mammalian cells, used with SNAP25B-12aa-mCit to measure FRET & dispers.
    Depositor
    Robert Zucker
    Article
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Insert
    Syntaxin 1A (Stx1a Rat)
    Use
    Tags
    12aa-linker and mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  16. CXXC1

    Plasmid
    #32045
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CXXC1 (CXXC1 Human)
    Use
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    CXXC domain (amino acids residues 161-222)
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 1081 - 1100 of 1204 results