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Showing: 10961 - 10980 of 14800 results

  1. HYG-TET-OFF – ubiquitin – LEU – 4FLAG

    Plasmid
    #193315
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    Ubiquitin - Leucine - 4FLAG N-terminal tagging
    Use
    Tags
    4FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    193315-plasmid-map-sequence-id-382367

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  2. HYG-TET-OFF – ubiquitin – LEU – 4MYC

    Plasmid
    #193316
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    Ubiquitin - Leucine - 4MYC N-terminal tagging
    Use
    Tags
    4MYC
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    193316-plasmid-map-sequence-id-382501

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  8. hPCNA-S228I

    Plasmid
    #190938
    Purpose
    untagged human PCNA with a hypomorphic disease-associated serine to isoleucine mutation at residue 228
    Depositor
    Brian Kelch
    Article
    Duffy et al J Mol Biol. 2016 Mar 27;428(6):1023-1040
    Insert
    Proliferating Cell Nuclear Antigen (PCNA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed serine 228 to isoleucine
    Promoter
    T7
    Available since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    190938-plasmid-map-sequence-id-376466

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  10. p663-UBC-miniTurbo-V5-HIPK4_IDG-K

    Plasmid
    #158185
    Purpose
    Gateway destination clone of HIPK4 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-HIPK4 (HIPK4 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    A670T, H813Y- please see depositor comment
    Promoter
    Ubiquitin
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    158185-plasmid-map-sequence-id-372366

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  11. FnRPR9-10

    Plasmid
    #182568
    Purpose
    Human fibronectin fragment, type-III repeats 9-10, R1374A, P1376A, R1379A mutant
    Depositor
    Timothy Springer
    Article
    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi
    Insert
    Human fibronectin fragment, type-III repeats 9-10
    Use
    Tags
    His and TEV cleavage site after His tag
    Expression
    Bacterial
    Mutation
    R1374A, P1376A, R1379A
    Promoter
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182568-plasmid-map-sequence-id-357182

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  12. FnRPR8-10

    Plasmid
    #182570
    Purpose
    Human fibronectin fragment, type-III repeats 8-10, R1374A, P1376A, R1379A mutant
    Depositor
    Timothy Springer
    Article
    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi
    Insert
    Human fibronectin fragment, type-III repeats 8-10
    Use
    Tags
    His and TEV cleavage site after His tag
    Expression
    Bacterial
    Mutation
    R1374A, P1376A, R1379A
    Promoter
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182570-plasmid-map-sequence-id-357184

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  13. mouse KANK4-Y801H Ankyrin Repeats pET151

    Plasmid
    #164058
    Purpose
    Expresses mouse KANK4 Ank domain -Y801H mutant in bacterial cells
    Depositor
    Ben Goult
    Insert
    Kidney Ankyrin Repeat-Containing Protein 4 Y801H mutant (Kank4 Mouse)
    Use
    Tags
    His-tag, TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    164058-plasmid-map-sequence-id-327667

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  17. pCEP4_HA-Halo-cmyc-GRM3 C127A

    Plasmid
    #140458
    Purpose
    Mammalian expression plasmid for N-terminal Halo- and c-myc-tagged mGluR3 C127A
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Halo, Signal/leader sequence from influenza hemag…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, Threonine 174 to alanine
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    140458-plasmid-map-sequence-id-292298

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Showing: 10961 - 10980 of 14800 results