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Plasmid#74244PurposeCancer-associated ASXL1 truncation mutant in mammalian expression vectorDepositorInsertASXL1 (ASXL1 Human)
UseTagsExpressionMammalianMutationContains ASXL1 amino acids 1-1304PromoterCMVAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBeta-lacZ-attBx2
Plasmid#124445PurposePlasmid for in vivo enhancer reporter assay. Plasmid contains a β-globin minimal promoter followed by a lacZ reporter gene derived from pRS16, with the entire reporter cassette flanked by attB sites.DepositorTypeEmpty backboneUseEnhancer reporter assay constructTagsLacZExpressionMammalianMutationPromoterbeta globin minimal promoterAvailable sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGW_PSD95.FingR-YFP
Plasmid#46299DepositorInsertPSD95.FingR (DLG4 Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag Jnk1a1
Plasmid#13798DepositorInsertJnk1a1 (MAPK8 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pssGFP-hCRT
Plasmid#80022PurposeMammalian expression of signal peptide-EGFP-humanCRTDepositorInsertCRT (CALR Human)
UseTagsEGFP and signal peptideExpressionMammalianMutationsignal peptide 17aa added before GFP-CRT, will ta…PromoterCMVAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLight1.2
Plasmid#111054PurposeExpresses dLight1.2 in mammalian cellsDepositorInsertdLight1.2
UseTagsFlag tagExpressionMammalianMutationPromoterCAGAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
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mTagRFP-T-Gamma-Tubulin-17
Plasmid#58017PurposeLocalization: Centrosomes, Excitation: 555, Emission: 584DepositorInsertGamma-Tubulin (TUBG1 Human)
UseTagsmTagRFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSicoR-Ef1a-mCh-Puro
Plasmid#31845DepositorTypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNA; Ef1alpha for mCherry-puromycinAvailable sinceJan. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-MCU-GFP
Plasmid#31732DepositorInsertMCU (MCU Human)
UseTagsGFPExpressionMammalianMutation*PromoterCMVAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-FLAG-Jhdm2a
Plasmid#38136DepositorInsertJhdm2a (Kdm3a Mouse)
UseTagsFLAGExpressionMammalianMutationprotein starts at aa115PromoterCMVAvailable sinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
BPK2101
Plasmid#65770PurposeBacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNADepositorInsertmammalian codon-optimized SaCas9, and SaCas9 gRNA
UseCRISPRTagsNLS-3xFlagExpressionBacterialMutationPromoterT7 (x2)Available sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-OTULIN (full-length, aa 1-352)
Plasmid#61464PurposeExpresses human OTULIN (full-length) in E. coli.DepositorInsertOTULIN (OTULIN Human)
UseTagsHis6-3CExpressionBacterialMutationPromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX458-ECFP
Plasmid#112220PurposeCloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFPDepositorInsertECFP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO_EGFP-RandE3
Plasmid#79872PurposeFor generating adeno-associated virus to express RandE3 under the EF1a promoter in a 'Cre-on' dependent manner.DepositorInsertGFP-RandE3
UseAAV, Cre/Lox, and Synthetic BiologyTagsGFP and HAExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti HRE mutant-Luc pGK Hygro
Plasmid#118707Purposereporter plasmid, HIF-responsive promoter (3xHRE) mutant- fused to firefly luciferaseDepositorInsertHREmutant-Luc
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLight1.4
Plasmid#111057PurposeExpresses dLight1.4 in mammalian cellsDepositorInsertdLight1.4
UseTagsFlag tagExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX ShcD-SH2
Plasmid#46488DepositorInsertShcD (SHC4 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 512-623PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
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mEmerald-Caveolin-N-10
Plasmid#54026PurposeLocalization: Membrane, Excitation: 487, Emission: 509DepositorInsertCaveolin (CAV1 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits