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Showing: 10941 - 10960 of 14800 results

  2. MARS-nGFP

    Plasmid
    #205234
    Purpose
    Expresses PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells
    Depositor
    Jeremy Baskin
    Insert
    PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody (PLEKHA5 Human)
    Use
    Tags
    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    CMV
    Available since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    205234-plasmid-map-sequence-id-404747

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  3. pCDNA3-2xMARS-PLD

    Plasmid
    #205237
    Purpose
    Expresses two repeats of PLEKAH5 aa 143-271 (K163A and R164A) fused to phospholipase D from Streptomyces sp. PMF
    Depositor
    Jeremy Baskin
    Inserts
    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    phospholipase D (PLD)
    Use
    Tags
    Nuclear Export Sequence and mCherry
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    CMV
    Available since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    205237-plasmid-map-sequence-id-404742

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  7. pET21b-hHNF4aORF-1

    Plasmid
    #206959
    Purpose
    The ORF of HNF4α (variant 2) cDNA amplified from the RT human liver RNA was cloned at the NheI/NotI sites of the pET21b(+), the bacterial expression vector.
    Depositor
    Reza Zolfaghari, Catharine Ross
    Insert
    HNF4a (Hepatocyte Nuclear Factor alpha4 Variant 2) (HNF4A Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    206959-plasmid-map-sequence-id-422372

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  10. MLKS2ec

    Plasmid
    #130855
    Purpose
    Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology
    Depositor
    Hank Bass
    Article
    Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10
    Insert
    MLKS2
    Use
    Gateway entry vector
    Tags
    eGFP-FLAG-HA
    Expression
    Mutation
    Promoter
    Available since
    Sept. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    130855-plasmid-map-sequence-id-255563

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  11. MLKS2exp

    Plasmid
    #130856
    Purpose
    Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology
    Depositor
    Hank Bass
    Article
    Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10
    Insert
    MLKS2
    Use
    Tags
    eGFP-FLAG-HA
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    130856-plasmid-map-sequence-id-255566

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  16. pMOD_C2911

    Plasmid
    #161764
    Purpose
    MODULE C plasmid compatible with the Voytas Plant Genome Engineering Tool kit with an Agrobacterium promoter rolD expressing the TREX2 exonuclease.
    Depositor
    Shaun Curtin
    Article
    Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6
    Insert
    rolD:TREX2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rolD
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    161764-plasmid-map-sequence-id-393578

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  18. BPTI-21

    Plasmid
    #195058
    Purpose
    simplyfied amino acid sequence by alanine scanning
    Depositor
    Peter Kim, Yutaka Kuroda
    Article
    Kuroda et al J Mol Biol. 2000 May 5;298(3):493-501. d
    Insert
    bovine pancreatic trypsin inhibitor
    Use
    Tags
    His tag and trpΔLE leader sequences
    Expression
    Bacterial
    Mutation
    21 amino acids are Alanine out of 58 amino acids
    Promoter
    T7 promotor
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195058-plasmid-map-sequence-id-388061

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  20. NAT-TET-OFF – ubiquitin – LEU – 4MYC

    Plasmid
    #193314
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    Ubiquitin - Leucine - 4MYC N-terminal tagging
    Use
    Tags
    4MYC
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    193314-plasmid-map-sequence-id-382366

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Showing: 10941 - 10960 of 14800 results