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pCMV4-3HA

Plasmid

#24165

Depositor

Warner Greene

Article

Sun et al Mol Cell Biol. 1996 Mar . 16(3):1058-65.

Type

Empty backbone

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
pT3-EF1a-c-Met

Plasmid

#31784

Depositor

Xin Chen

Article

Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

Insert

Met (MET Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP:HA-mTREK-1

Plasmid

#224773

Purpose

Mammalian expression vector for generating mouse TREK-1

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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HBV 1.3-mer P-null replicon

Plasmid

#65462

Purpose

containing 1.3 units of the P-null HBV genome (subtype ayw)

Depositor

Wang-Shick Ryu

Article

Wang et al J Virol. 2009 Jun;83(11):5815-24. doi: 1

Insert

1.3 units of the P-null HBV genome

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pET21a-MetRS-His

Plasmid

#124115

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Methionine--tRNA ligase (metG Escherichia coli)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pICSL80005

Plasmid

#50322

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, variant of turboGFP codon-optimised for plants (Pontellina plumata)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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ER-mGreenLantern

Plasmid

#164463

Purpose

Localization and retention of mGreenLantern fluorescent protein in the ER

Depositor

Gregory Petsko

Article

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Use

Tags

Calreticulin and KDEL

Expression

Mammalian

Mutation

Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

Promoter

CMV

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-CMP-PE-V1

Plasmid

#202769

Purpose

Expresses CMP-PE-V1 in mammalian cells for prime editing

Depositor

Kyoungmi Kim

Article

Park et al Genome Biol. 2021 Jun 3;22(1):170. doi:

Insert

CMP-PE-V1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV14-3X-Flag-SARS-CoV-2 S

Plasmid

#145780

Purpose

Over expression of SARS-CoV-2 S (spike) in mammalian cell line

Depositor

Zhaohui Qian

Article

Ou et al Nat Commun. 2020 Mar 27;11(1):1620. doi:

Insert

SARS-CoV-2 S (S SARS CoV 2)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Codon optimized and C-terminal 19 amino acids del…

Promoter

CMV

Available since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-PP7v3

Plasmid

#107230

Purpose

Expresses 24x optimized PP7 stem loops (version 3) in bacterial cells

Depositor

Jeffrey Chao

Article

Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

Insert

24xPP7v3

Use

Tags

3x FLAG-tag and P2A site

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pTORKm43GWF

Plasmid

#112899

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mWasabi.

Depositor

Sebastian Schornack

Article

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Tags

Expression

Mutation

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pnlacZ-ACN

Plasmid

#20304

Depositor

Mario Capecchi

Article

Wu et al Nat Protoc. 2008 . 3(6):1056-76.

Insert

nlacZ-ACN

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2009

Availability

Academic Institutions and Nonprofits only

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UAS-u(M)Cas9

Plasmid

#127384

Purpose

Expresses Cas9 at medium level

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

uORF(M)-Cas9

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Puro

Plasmid

#167911

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Nan Cher Yeo, Alejandro Chavez

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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FUW-OSKM

Plasmid

#20328

Purpose

Lentiviral plasmid expressing mouse Oct4, Sox2, Klf4 and cMyc for iPS cell generation

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc (Myc, Sox2, Pou5f1, Klf4 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pST39-pNL29

Plasmid

#91696

Purpose

Expresses Mega GVs in E.Coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Protoc. 2017 Oct;12(10):2050-2080. d

Insert

Mega GV gene cluster (from pNL29) in pST39 backbone

Use

Tags

No tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pNX3b-Myc13

Plasmid

#78367

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

13xMyc
hygMX6

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pLR16-Phes*

Plasmid

#98783

Purpose

Suicide vector for allelic exchange in Listeria utilizing an engineered counterselection marker for Listeria monocytogenes based on a mutated phenylalanyl-tRNA synthetase gene (pheS*).

Depositor

Anat Herskovits

Article

Argov et al Appl Environ Microbiol. 2017 Mar 2;83(6)

Insert

pheS

Use

Tags

Expression

Bacterial

Mutation

A309G

Promoter

Available since

Jan. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SC35-cMyc

Plasmid

#44721

Depositor

Kathleen Scotto

Article

Shi et al Mol Cell Biol. 2008 Jan;28(2):883-95. Ep

Insert

SC35 (SRSF2 Human)

Use

Tags

his and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 29, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG_smFP HA

Plasmid

#59759

Purpose

encodes multiple copies of an epitope tag HA

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _HA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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