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pcDNA3.1-HA-UBA6

Plasmid

#136995

Purpose

Expresses human UBA6 in mammalian cells

Depositor

Marcus Groettrup

Article

Pelzer et al J Biol Chem. 2007 Aug 10;282(32):23010-4

Insert

UBA6 (UBA6 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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mNG2(1-10)_tdTomato

Plasmid

#184169

Purpose

Lentiviral vector for expression and selection of the mNG2(1-10) split fluorescent protein.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mNG2(1-10)
tdTomato

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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BII-ChBtW-iresFUCCI

Plasmid

#133371

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

Clover-Geminin-IRES-mKO2-Cdt1

Use

Tags

Flag

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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GZnP2 in pcDNA3.1+

Plasmid

#161739

Purpose

Cytosolic fluorescent reporter for Zn²⁺ (Kd = 352 pM, ex/em = 488 nm/515 nm)

Depositor

Yan Qin

Article

Fudge et al ACS Chem Biol. 2018 Jul 20;13(7):1897-19

Insert

GZnP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PB1-AG(Y63L)-LOV2

Plasmid

#178876

Purpose

Mammalian expression of PB1-AzamiGreen(Y63L)-LOV2

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen(Y63L)-LOV2

Use

Tags

Expression

Mammalian

Mutation

AzamiGreen: Y63L

Promoter

CAG

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pGene-TAP-GBP1

Plasmid

#138087

Purpose

Plasmid with TAP-GBP1 ORF

Depositor

Eva Frickel

Article

Fisch et al EMBO J. 2019 Jul 1;38(13):e100926. doi:

Insert

human GBP1 (GBP1 Human)

Use

Tags

Tandem affinity purification (TAP) tag

Expression

Mammalian

Mutation

Promoter

Gal4-E1b

Available since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pSL0426 (CRISPR with spc3)

Plasmid

#130642

Purpose

Expresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA with spacer 3 from the native CRISPR.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

CRISPR(spacer-3)

Use

CRISPR; Transposon

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pZIP-hCMV-ZsGreen-P2A-Puro-NT4

Plasmid

#123328

Purpose

Lentiviral vector for expression of non-targeting control shRNA #4 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insert

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

non-targeting control shRNA embedded in miR30 backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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RFP-(SUMO FAAA)3

Plasmid

#130703

Purpose

Mammalian expression plasmid for negative control of mCherry-(SUMO)3.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

3 copies of human SUMO1 separated by (GGS)4

Use

Tags

Expression

Mammalian

Mutation

FKVK sequence in each SUMO1 module changed to FAAA

Promoter

CMV

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pTopo_Ex2Donor_pPGK-PURO

Plasmid

#198862

Purpose

Donor template for CRISPR/Cas9 targeting of TERT Ex2

Depositor

Agnel Sfeir

Article

Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

Insert

PURO

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCiPS-NIFV

Plasmid

#110807

Purpose

Non-Integrating Foamy Virus pol-expressing helper plasmid with mutations in the DDE catalyic core motif of the integrase sequence, remaining as unintegated episomes in transduced cells

Depositor

David Russell

Article

Deyle et al J Virol. 2010 Sep;84(18):9341-9. doi: 10

Insert

Pol

Use

Tags

Expression

Mammalian

Mutation

2 mutations in the DDE catalyic core motif of the…

Promoter

CMV

Available since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3-TYK2

Plasmid

#139354

Purpose

Expression of TYK2 protein fused with GFP

Depositor

Sandra Pellegrini

Article

Ragimbeau et al J Biol Chem. 2001 Aug 17;276(33):30812-8

Insert

TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV/3' Box_(GLuc)_TOP1

Plasmid

#68434

Purpose

Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

TOP1 construct

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ141D-gRNA2.1

Plasmid

#167161

Purpose

Express single gRNA with gRNA2.1 scaffold (with four MS2 binding sites) under OsU3 promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

OsU3

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV4a-SIRT6-FLAG

Plasmid

#102322

Purpose

Human SIRT6 with FLAG tag for mammalian expression

Depositor

Hening Lin

Article

Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

Insert

SIRT6 (SIRT6 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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mLPx-shZMPSTE24

Plasmid

#69067

Purpose

encodes a shRNA against the protease ZMPSTE24

Depositor

Gerardo Ferbeyre

Article

Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d

Insert

shRNA against ZMPSTE24 (ZMPSTE24 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-mCherry-CyrCR1

Plasmid

#128768

Purpose

Expresses mCherry in mammalian cells. ~200 nt of Cyrano inserted in 3'UTR with miR-7 site replaced by miR-17 site.

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

Cyrano conserved region (CR1) with intact miR-7 site (OIP5-AS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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BII-ChPtW-PLAGL2

Plasmid

#133380

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

PLAGL2 (PLAGL2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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KA1775_pPBCAG-rtTAM2-IH

Plasmid

#124167

Purpose

piggyBac-based expression of rtTA-M2

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

rtTAM2-IRES-Hph

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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mTagRFP-pBAD

Plasmid

#54539

Purpose

Localization: Protein Expression Vector , Excitation: 555, Emission: 584

Depositor

Michael Davidson, Dorus Gadella, Dmitriy Chudakov

Article

Merzlyak et al Nat Methods. 2007 Jul;4(7):555-7. Epub 2

Type

Empty backbone

Use

Tags

6xHis and mTagRFP

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits