Addgene

We narrowed to 14,800 results for: LEA

Showing: 10921 - 10940 of 14800 results

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pET28a-hfYFP-TEV-Bcx

Plasmid

#191195

Purpose

Fusion of hfYFP and Bacillus circulans xylanase separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

hfYFP & Bcx fusion

Use

Tags

6xHis and Bacillus circulans xylanase

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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L469R USP7 catalytic

Plasmid

#227212

Purpose

Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

L469R; aa 207-560

Promoter

T7-promoter

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pRSET_SynPspA

Plasmid

#223812

Purpose

Bacterial expression of SynPspA with N-terminal 10xHis-tag and EK cleavage site

Depositor

Carsten Sachse

Article

Junglas et al Cell. 2021 Jul 8;184(14):3674-3688.e18.

Insert

SynPspA

Use

Tags

10xHis and NusA

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-PKP3(305-797)

Plasmid

#202599

Purpose

Expresses ARM domain of plakophilin3

Depositor

Tina Izard

Article

Gupta et al Int J Mol Sci. 2023 May 29;24(11):9458.

Insert

plakophilin3 (PKP3 Human)

Use

Tags

His8 tag with precision protease cleavage site

Expression

Bacterial

Mutation

Deleted amino acids 1 to 304

Promoter

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD-AE

Plasmid

#226968

Purpose

Expressing Olsenella uli indoleacetate decarboxylase activating enzyme (IAD-AE) in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD-AE

Use

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD

Plasmid

#226967

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD

Use

Tags

His6 (on backbone)

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET24a_SMOX

Plasmid

#186345

Purpose

C-terminal His-Tag and a TEV cleaveage site, to transform into E. coli BL21 (DE3) cells

Depositor

Sandra Gabelli

Article

Dunston et al Acta Naturae. 2020 Jul-Sep;12(3):140-144

Insert

Spermine oxidase (SMOX Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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L299A USP7 catalytic

Plasmid

#227213

Purpose

Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

L299A; aa 207-560

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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V485G USP7 catalytic

Plasmid

#227209

Purpose

Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

V485G; aa 207-560

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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P273H USP7 catalytic

Plasmid

#227208

Purpose

Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

P273H; aa 207-560

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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Y331H USP7 catalytic

Plasmid

#227205

Purpose

Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

Y331H; aa 207-560

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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M225I USP7 catalytic

Plasmid

#227206

Purpose

Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

M225I; aa 207-560

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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USP7 N418S catalytic

Plasmid

#227202

Purpose

Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

N418Sl; aa 207-560

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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K420E USP7 catalytic

Plasmid

#227210

Purpose

Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

K420E; aa 207-560

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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USP7 G392D catalytic

Plasmid

#227203

Purpose

Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

G392D; aa 207-560

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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E345K USP7 catalytic

Plasmid

#227211

Purpose

Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

E345K; aa 207-560

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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L373F USP7 catalytic

Plasmid

#227207

Purpose

Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

L373F; aa 207-560

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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G392A USP7 catalytic

Plasmid

#227204

Purpose

Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

G392A; aa 207-560

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a:ChrH

Plasmid

#225084

Purpose

Expresses multinuclear non-heme iron dependent oxidative enzyme ChrH in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrH

Use

Tags

His6-Thrombin

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLVUT-spGFP

Plasmid

#225486

Purpose

Tet-on lentiviral vector for controlled expression of secretable and permeable EGFP

Depositor

Robert Gross

Article

Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d

Insert

Igκ leader and TAT peptide

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Human ubiquitin C

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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