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GFP-Sharpin

Plasmid

#195092

Purpose

Gene Overexpression of Human SHARPIN

Depositor

Jukka Westermarck

Publication

Butt et al J Cell Sci. 2022 Oct 15;135(20):jcs26062

Insert

SHARPIN (SHARPIN Human)

Expression

Mammalian

Available since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAD6

Plasmid

#134349

Purpose

E. coli mfd gene cloned into pSKB2 with an N-terminal, ppx-cleavable His6 tag

Depositor

Seth Darst

Publication

Deaconescu et al Acta Crystallogr Sect F Struct Biol Crys

Insert

mfd (mfd E. coli)

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0328

Plasmid

#133489

Purpose

Expression of nucleo-cytoplasmic mCherry

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(act1)-mCherry-terminator(tdh1)

Expression

Yeast

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAV0749

Plasmid

#133483

Purpose

Expression in fission yeast from promoter ptdh1 , integrated at ura4 locus

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(tdh1)-sfGFP-terminator(ScCYC1)

Expression

Yeast

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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tISR

Plasmid

#122664

Purpose

Express tISR protein in E. coli

Depositor

Robert Cohen

Publication

Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Insert

tISR (OCLM Human)

Tags

His-tag

Expression

Bacterial

Promoter

T7 promoter

Available since

July 17, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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FFiBlast p5

Plasmid

#68470

Purpose

Inverted and floxed blasticidin resistance

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Blasticidin Resistance

Use

Cre/Lox

Expression

Mammalian

Mutation

Inverted

Promoter

LTR

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pSicoR-RNF40i2

Plasmid

#59595

Purpose

Expression of shRNA against human RNF40

Depositor

Miguel Ramalho-Santos

Publication

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

RNF40 (RNF40 Human)

Use

RNAi

Expression

Mammalian

Promoter

u

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
pET21a.MJ0135

Plasmid

#11412

Depositor

Sung-Hou Kim

Publication

Lai et al Structure. 2000 Aug 15. 8(8):897-904.

Insert

RNase HII (MJ_RS00700 M. jannaschii)

Expression

Bacterial

Promoter

T7

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

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[#GS32-LV] EF1a-MitoTRACER-Donor

Plasmid

#253760

Purpose

Lentiviral MitoTRACER donor construct under EF1a Promoter

Depositor

Simon Grelet

Publication

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

MitoTRACER-Donor

Tags

GFP11 and HA Tag

Promoter

EF1a

Available since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-20nm-EBFP2

Plasmid

#231185

Purpose

Expresses a synthetic linker, tagged with EBFP2, Stabilizing the ER-mitochondrial distance at 20 nm

Depositor

Dmitry Lim

Publication

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

20nm-EBFP2

Expression

Mammalian

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p15A-OptoCre-knt-P-R

Plasmid

#189849

Purpose

Cre-inducible activation of knt for kanamycin resistance.

Depositor

Mary Dunlop

Publication

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Insert

P-loxP-TT-loxP-R-knt

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

P (T7 phage gene 10)

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Sharpin

Plasmid

#195092

Purpose

Gene Overexpression of Human SHARPIN

Depositor

Jukka Westermarck

Publication

Butt et al J Cell Sci. 2022 Oct 15;135(20):jcs26062

Insert

SHARPIN (SHARPIN Human)

Expression

Mammalian

Available since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAD6

Plasmid

#134349

Purpose

E. coli mfd gene cloned into pSKB2 with an N-terminal, ppx-cleavable His6 tag

Depositor

Seth Darst

Publication

Deaconescu et al Acta Crystallogr Sect F Struct Biol Crys

Insert

mfd (mfd E. coli)

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV0328

Plasmid

#133489

Purpose

Expression of nucleo-cytoplasmic mCherry

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(act1)-mCherry-terminator(tdh1)

Expression

Yeast

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pAV0749

Plasmid

#133483

Purpose

Expression in fission yeast from promoter ptdh1 , integrated at ura4 locus

Depositor

Sophie Martin

Publication

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(tdh1)-sfGFP-terminator(ScCYC1)

Expression

Yeast

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
tISR

Plasmid

#122664

Purpose

Express tISR protein in E. coli

Depositor

Robert Cohen

Publication

Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Insert

tISR (OCLM Human)

Tags

His-tag

Expression

Bacterial

Promoter

T7 promoter

Available since

July 17, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
FFiBlast p5

Plasmid

#68470

Purpose

Inverted and floxed blasticidin resistance

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Blasticidin Resistance

Use

Cre/Lox

Expression

Mammalian

Mutation

Inverted

Promoter

LTR

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSicoR-RNF40i2

Plasmid

#59595

Purpose

Expression of shRNA against human RNF40

Depositor

Miguel Ramalho-Santos

Publication

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

RNF40 (RNF40 Human)

Use

RNAi

Expression

Mammalian

Promoter

u

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only
pET21a.MJ0135

Plasmid

#11412

Depositor

Sung-Hou Kim

Publication

Lai et al Structure. 2000 Aug 15. 8(8):897-904.

Insert

RNase HII (MJ_RS00700 M. jannaschii)

Expression

Bacterial

Promoter

T7

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

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pTH10

Plasmid

#188783

Purpose

Carries a NAT marker and C. albicans RBT5 promoter in a pGEM-T backbone

Depositor

Aaron Mitchell

Insert

RBT5 promoter

Use

Unspecified

Available since

Sept. 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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