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Plasmid#89062PurposeAAV-gRNA targeting the murine Apob geneDepositorInsertEmGFP
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterChicken beta actin with partial CMV enhancerAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-PRAS40 AA
Plasmid#86759PurposeN-terminal HA-tagged protein expression in mammalian cellsDepositorInserthuman PRAS40 (AKT1S1 Human)
UseTagsHAExpressionMammalianMutationAA (S183A, S221A)PromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-PRAS40 F129A
Plasmid#86763PurposeN-terminal HA-tagged protein expression in mammalian cellsDepositorInserthuman PRAS40 (AKT1S1 Human)
UseTagsHAExpressionMammalianMutationF129APromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-PRAS40 W10A
Plasmid#86761PurposeN-terminal HA-tagged protein expression in mammalian cellsDepositorInserthuman PRAS40 (AKT1S1 Human)
UseTagsHAExpressionMammalianMutationW10APromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTSara-N748D
Plasmid#60726PurposeExpresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation N748D. Contains a constitutive araC ORFDepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…PromoterAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO shMKP7.v1 puro
Plasmid#87794PurposeExpresses an inducible short hairpin targeting human MKP7 sequenceDepositorInsertshMKP7 (DUSP16 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE tightAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
hFlt1 V767A
Plasmid#86038Purposehuman VEGFR1 with V to A mutation at position 767DepositorInserthuman Flt1 V767A (FLT1 Human)
UseTagsFLAG, HA (please see depositor comments below), a…ExpressionMammalianMutationV767APromoterCMVAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO shDUSP19 puro
Plasmid#87796PurposeExpresses an inducible short hairpin targeting human DUSP19 sequenceDepositorInsertshDUSP19 (DUSP19 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE tightAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hs-3xHA-deltaBBox-LIN-41
Plasmid#52719Purposeretroviral expression of human LIN-41 / TRIM71 containing deletion of the BBox domain and 3 N-terminal HA tagsDepositorInsertLIN-41 (TRIM71 Human)
UseRetroviralTags3xHAExpressionMammalianMutationdeleted amino acids 194-320PromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hs-3xHA-deltaFilamin-LIN-41
Plasmid#52721Purposeretroviral expression of human LIN-41 / TRIM71 containing deletion of the Filamin domain and 3 N-terminal HA tagsDepositorInsertLIN-41 (TRIM71 Human)
UseRetroviralTags3xHAExpressionMammalianMutationdeleted amino acids 483–583PromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
2.3 hA33 luciferase
Plasmid#68148Purposeluciferase reporter for hA33 promoterDepositorInsertA33 (GPA33 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationstarts at -2270 relative to ATGPromoterhuman GPA33 promoterAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
CIP2Aprom27bp-pGL4.10Luc
Plasmid#60876PurposeLuc reporter driven by 27 bp of the CIP2A promoterDepositorInsert27 bp promoter fragment of Cancerous Inhibitor of PP2A (CIP2A Human)
UseLuciferase; PromoterlessTagsExpressionMutationPromoterAvailable sinceApril 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-WRAP53beta-N-terminus
Plasmid#64677Purposeexpresses only N terminal o WRAP53betaDepositorInsertWD40-encoding RNA antisense to P53 (WRAP53 Human)
UseTagsGFPExpressionMammalianMutationOnly N terminus amino acids 1-160PromoterCMVAvailable sinceMay 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-WRAP53beta-C-terminus
Plasmid#64679Purposeexpresses only C terminus of WRAP53betaDepositorInsertWD40-encoding RNA antisense to P53 (WRAP53 Human)
UseTagsGFPExpressionMammalianMutationonly C terminus amino acids 500-548PromoterCMVAvailable sinceMay 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFB-Flag-MyoD(QQQ)-E12
Plasmid#26003DepositorInsertMyoD(QQQ)-E12 Heterodimer Fusion (Myod1 Mouse)
UseBaculovirus transfer vectorTagsFlagExpressionMutationChanged Lysine 99 to Glutamine; Lysine 102 to Glu…PromoterAvailable sinceSept. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
Alt AlphaPS3
Plasmid#14070DepositorInsertDrosphila Alt Alpha PS3 Integrin (scb Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Sema4D RNAiR
Plasmid#51600Purposeexpresses RNAi-resistant mouse Sema4D with N-terminal myc tagDepositorInsertSema4D (Sema4d Mouse)
UseTagsmycExpressionMammalianMutationsilent mutations between amino acids (approx 275-…PromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP-TRAF4(K190R)
Plasmid#58319PurposeEGFP-tagged TRAF4 K190R mutantDepositorInsertTNF receptor associated factor 4 (Traf4 Mouse)
UseTagsGFPExpressionMammalianMutationK 190 to RPromoterCMVAvailable sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
3625 pSP72 Bim (genomic)
Plasmid#8805DepositorInsertBim genomic (Bcl2l11 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only