Showing: 10841 - 10860 of 20580 results
-
Plasmid#122132PurposeExpress a dCas9, 10X GCN4, and mCherry cassette from the TRE3G promoterDepositorInsertdCas9-10X GCN4-P2A-mCherry
UseCRISPR and LentiviralTagsExpressionMammalianMutationdCas9 D10A & H840APromoterTRE3GAvailabilityAcademic Institutions and Nonprofits only -
pHR-PGK-scFv GCN4-sfGFP-VP64
Plasmid#122133PurposeExpress a scFv which binds to GCN4, sfGFP and VP64 cassette from the PGK promoterDepositorInsertscFv GCN4-sfGFP-VP64
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterPGKAvailabilityAcademic Institutions and Nonprofits only -
pBA950
Plasmid#122239PurposeCROP-seq vector optimized for sgRNA expression and CRISPRi activity. This vector expresses a eGFP-NT2 sgRNA with modified U6 promotor and sgRNA constant region.DepositorInsertBFP selectable marker, plus a modified mU6 promoter and sgRNA constant region for optimized CRISPRi activity
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCAG-hMbCas12a-NLS(nucleoplasmin)-3xHA (AAS2134)
Plasmid#114090PurposeCAG promoter expression plasmid for human codon optimized MbCas12a nuclease with C-terminal NLS and HA tagDepositorInserthuman codon optimized MbCas12a
UseTagsNLS(nucleoplasmin) and 3xHAExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTE3330
Plasmid#107534PurposeExpresses Lb crRNA and human codon optimized LbCpf1(RVR mutant) in mammalian cells.DepositorInsertsLb crRNA
hLbCpf1(RVR mutant)
UseTags3xHA and NLSExpressionMammalianMutationG532R, K538V, Y542RPromoterCMV and human U6AvailabilityAcademic Institutions and Nonprofits only -
pTE3327
Plasmid#107535PurposeExpresses As crRNA and human codon optimized AsCpf1(RVR mutant) in mammalian cells.DepositorInsertsAs crRNA
hAsCpf1(RVR mutant)
UseTags3xHA, NLS, and SV40 NLSExpressionMammalianMutationS542R, K548V, N552RPromoterCMV and human U6AvailabilityAcademic Institutions and Nonprofits only -
pTE3331
Plasmid#107536PurposeExpresses Fn crRNA and human codon optimized FnCpf1(RVR mutant) in mammalian cells.DepositorInsertsFn crRNA
hFnCpf1(RVR mutant)
UseTags3xHA and NLSExpressionMammalianMutationN607R, K613V, N617RPromoterCMV and human U6AvailabilityAcademic Institutions and Nonprofits only -
pTE3334
Plasmid#107537PurposeExpresses Mb crRNA and human codon optimized MbCpf1(RVR mutant) in mammalian cells.DepositorInsertsMb crRNA
hMbCpf1(RVR mutant)
UseTags3xHA and NLSExpressionMammalianMutationN576R, K582V, N586RPromoterCMV and human U6AvailabilityAcademic Institutions and Nonprofits only -
pTE3329
Plasmid#107538PurposeExpresses Lb crRNA and human codon optimized LbCpf1(RR mutant) in mammalian cells.DepositorInsertsLb crRNA
hLbCpf1(RR mutant)
UseTags3xHA, NLS, and SV40 NLSExpressionMammalianMutationG532R, K595RPromoterCMV and human U6AvailabilityAcademic Institutions and Nonprofits only -
pTE3328
Plasmid#107539PurposeExpresses As crRNA and human codon optimized AsCpf1(RR mutant) in mammalian cells.DepositorInsertsAs crRNA
hAsCpf1(RR mutant)
UseTags3xHA, NLS, and SV40 NLSExpressionMammalianMutationS542R, K607RPromoterCMV and human U6AvailabilityAcademic Institutions and Nonprofits only -
pTE3333
Plasmid#107540PurposeExpresses Fn crRNA and human codon optimized FnCpf1(RR mutant) in mammalian cells.DepositorInsertsFn crRNA
hFnCpf1(RR mutant)
UseTags3xHA and NLSExpressionMammalianMutationN607R, K671RPromoterCMV and human U6AvailabilityAcademic Institutions and Nonprofits only -
pTE3336
Plasmid#107541PurposeExpresses Mb crRNA and human codon optimized MbCpf1(RR mutant) in mammalian cells.DepositorInsertsMb crRNA
hMbCpf1(RR mutant)
UseTags3xHA and NLSExpressionMammalianMutationN576R, K637RPromoterCMV and human U6AvailabilityAcademic Institutions and Nonprofits only -
pJEP310-pAAV-Mecp2P-SaCas9-P2A-HAFLAGHA-KASH-pA
Plasmid#113687PurposeSaCas9 driven by the neuron specific promoter Mecp2P. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACS.DepositorInsertSaCas9 (NEWENTRY )
UseAAV and CRISPRTagsFlag, HAx2, KASH, and NLSExpressionMutationPromoterCytomegalo Virus(CMV)AvailabilityAcademic Institutions and Nonprofits only -
pJEP312-pAAV-CMV-SaCas9-P2A-HAFLAGHA-KASH-pA
Plasmid#113689PurposeSaCas9 driven by CMV. SaCas9 is followed by the self cleaving P2A sequence, several tags, and then the KASH transmembrane domain to enable FACSDepositorInsertSaCas9 (NEWENTRY )
UseAAV and CRISPRTagsFlag, HAx2, KASH, and NLSExpressionMutationPromoterCytomegalo Virus(CMV)AvailabilityAcademic Institutions and Nonprofits only -
pJEP314-pAAV-U6SaCas9gRNA(SapI)-CMV-SaCas9-DIO-pA
Plasmid#113691PurposeU6 driven SaCas9 gRNA expression cassette followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.DepositorInsertSaCas9
UseAAV, CRISPR, and Cre/LoxTagsNLSExpressionMutationPromoterCytomegalo Virus(CMV)AvailabilityAcademic Institutions and Nonprofits only -
pJEP315-pAAV-U6SaCas9gRNA(CREB)-CMV-SaCas9-DIO-pA
Plasmid#113692PurposeU6 SaCas9 gRNA expression cassette containing a gRNA targeting the CREB gene, followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.DepositorUseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsNLSExpressionMutationPromoterCytomegalo Virus(CMV)AvailabilityAcademic Institutions and Nonprofits only -
pJEP321-pAAV-FullU6TO-SaCas9gRNAi(SapI)-CMV-TetR-P2A-GFP-KASH-WPRE-shortPA Empty Cassette
Plasmid#113698PurposeDox-inducible U6 SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.DepositorInsertsSaCas9 gRNA Cassete
GFP
UseAAV and CRISPRTagsKASHExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJEP322-pAAV-FullU6TO-SaCa9sgRNAi(emx1-sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA
Plasmid#113699PurposeDox-inducible U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.DepositorInsertsSaCas9 gRNA Cassete
GFP
UseAAV and CRISPRTagsKASHExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJEP323-pAAV-FullH1TO-SaCa9gRNAi(SapI)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA Empty Cassette
Plasmid#113700PurposeDox-inducible H1 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in a gRNAs.DepositorInsertsSaCas9 gRNA Cassete
GFP
UseAAV and CRISPRTagsKASHExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJEP324-pAAV-FullH1TO-SaCa9sgRNAi(emx1sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA
Plasmid#113701PurposeDox-inducible H1 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.DepositorInsertsSaCas9 gRNA Cassete
GFP
UseAAV and CRISPRTagsKASHExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 10841 - 10860 of 20580 results