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Addgene
Showing: 10841 - 10860 of 14149 results

  1. p5A7-T

    Plasmid
    #92020
    Purpose
    Expresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain and a second in the C-terminal domain, anhydrotetracycline inducible TEV protease
    Depositor
    Brian Pfleger
    Article
    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do
    Inserts
    TALE targeting lac operator with N and C-terminal TEV protease cleavage sites
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Expression
    Mutation
    S219V
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    92020-plasmid-map-sequence-id-190036

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  2. p5B3-T

    Plasmid
    #92021
    Purpose
    Expresses TALE targeted to lysA promoter with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease
    Depositor
    Brian Pfleger
    Article
    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do
    Inserts
    TALE targeting lysA with N-terminal TEV protease cleavage site
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Expression
    Mutation
    S219V
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    92021-plasmid-map-sequence-id-181444

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  3. p5C3-T

    Plasmid
    #92022
    Purpose
    Expresses TALE targeted to sucA with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease
    Depositor
    Brian Pfleger
    Article
    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do
    Inserts
    TALE targeting sucA with N-terminal TEV protease cleavage site
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Expression
    Mutation
    S219V
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    92022-plasmid-map-sequence-id-190017

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  4. AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep

    Plasmid
    #92099
    Purpose
    Doyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.
    Depositor
    Yannick Doyon
    Article
    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15
    Type
    Empty backbone
    Use
    CRISPR and TALEN; Zfn
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tet-On 3G Bidirectional
    Availability
    Academic Institutions and Nonprofits only
    92099-plasmid-map-sequence-id-180428

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  8. pLTR.gp140/EGFP.Revdelta38/DsRed

    Plasmid
    #115775
    Purpose
    HIV splicing reporter allows the assessment of the effect of LRAs on HIV-1 transcription and splicing.
    Depositor
    Damian Purcell
    Article
    Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37
    Insert
    Envelope fused to EGFP (gp140-EGFP) and non-functional Rev protein fused to DsRed fluorescent protein (Rev-delta38-DsRed).
    Use
    Tags
    EGFP; dsReD
    Expression
    Mammalian
    Mutation
    gp140 uncleaved (1190-91aa, KR > TG; truncated…
    Promoter
    HIV-1 (LTR)
    Availability
    Academic Institutions and Nonprofits only
    115775-plasmid-map-sequence-id-224438

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Showing: 10841 - 10860 of 14149 results