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Plasmid#10708DepositorInsertFKHRL1 (FOXO3 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pTCF158 Human beta-2 microglobulin
Plasmid#180457PurposeE. coli expression of class I MHC light chainDepositorInsertHuman beta-2 microglobulin
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
FRB-mCherry-Sec61β
Plasmid#186574PurposeExpression of FRB-mCherry-tagged ER protein Sec61beta in mammalian cellsDepositorInsertSEC61B (SEC61B Human)
UseTagsFRB-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA5/FRT/TO V5 HSPA8
Plasmid#19514DepositorInsertheat shock 70kDa protein 8 (HSPA8 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pλPR cI-Elysis
Plasmid#176891PurposeBacterial expression plasmids for production of E. coli bacterial ghost (BG) capsulesDepositorInsertLysis E gene from PhiX174 and lambda PR-cI857 regulatory system
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ConVERGD-GC8m-W3SL
Plasmid#218762PurposeProvides AND intersectional (Cre+Flp) expression of GCaMP8mDepositorInsertGCaMP8m
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
FNLCR CRISPRa Cell Surface and FNLCR CRISPRa Soluble Libraries
Pooled Library#1000000228DepositorAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
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GreenT-EC - Display - CyRFP1
Plasmid#193943PurposeMammalian expression vector for a low affinity calcium indicator exposed to the cell surfaceDepositorInsertGreenT-EC
UseTagsIgk_secretion - HA-Flag and PDGFRExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCCLc-MND-A0201-SABR-Backbone
Plasmid#119050Purpose3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterMNDAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pERM2-nhpSer
Plasmid#201922PurposeEnables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.DepositorInsertsSepRS(2)
Sep-tRNA v2.0
SerB
EF-Sep
UseTagsNoneExpressionBacterialMutationE412P, E414F, T417K, P495M, I496W, F529S and H67R…PromoterGlnS (constitutive), OXB20 (constitutive), lpp (c…Available sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pODN-mNG-RHOA
Plasmid#183836PurposeRepair template for the N-terminal tagging of RhoA with mNeonGreen in human cells using CRISPR/Cas9.DepositorInsertRHOA homology arms with mNeonGreen-linker (RHOA Human)
UseCRISPR; Donor templateTagsmNeonGreen-linkerExpressionMammalianMutationHomology arms contain point mutations to remove t…PromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pDisplay-GRAB-rDA3h-IRES-EGFP-CAAX
Plasmid#208697PurposeExpresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h and a membrane-localized EGFP in mammalian cellsDepositorInsertGPCR activation based dopamine (DA) sensor GRAB_rDA3h
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_NTD-GFP-HiBiT
Plasmid#198317PurposeLoads HiBiT-tagged GFP into PVCs using Pdp1 packaging domain; also contains PnfDepositorInsertPdp1_NTD-GFP-HiBiT
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
AXBEv2
Plasmid#204606PurposeMammalian expression of evolved adenine transversion base editor AXBEv2DepositorInsertbpNLS-TadA-8e-nSpCas9-mAAG (R165E/Y179F)-bpNLS-P2A-GFP
UseCRISPRTags6*HisExpressionMammalianMutationmAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)PromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMXs-gw
Plasmid#18656DepositorInsertGateway cassette A
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
STICR_IndexE
Pooled Library#180483PurposeHigh Complexity Lentiviral Barcode LibraryDepositorExpressionMammalianSpeciesSyntheticUseLentiviralAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRPN265NB-LaG16-MNase
Plasmid#170978PurposeExpresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & RunDepositorInsertGlutathione-S-Transferase/LaG16/MNase
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only