Addgene

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pXP822

Plasmid

#46064

Depositor

Nancy DaSilva

Article

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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Ex1(-120/+3)

Plasmid

#30492

Depositor

Gerhart Ryffel

Article

Drews et al BMC Dev Biol. 2011 . 11():5.

Insert

lhx1 promoter

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pRB35

Plasmid

#72866

Purpose

template base vector for N-terminal tagging, constitutive Puromycin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

base vector for N-terminal tags, Puro

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pAC1822_pCR8-dCasRx

Plasmid

#118656

Purpose

Gateway Donor; dCasRx ORF; No promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

dCasRx

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

No promoter

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pRB36

Plasmid

#72861

Purpose

template base vector for N-terminal tagging, constitutive Blasticidin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

base vector for introduction of tags

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pYS104

Plasmid

#1190

Depositor

Susan Lindquist

Article

Patino et al Science 1996 Aug 2;273(5275):622-6.

Insert

Hsp104 (HSP104 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

wild-type Hsp104 sequence with 588bp of 5' p…

Promoter

Available since

June 23, 2005

Availability

Academic Institutions and Nonprofits only
circRAJ31

Plasmid

#69926

Purpose

Expresses RAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator.

Depositor

Alfonso Jaramillo

Article

Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

Insert

plTet-circRAJ31

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PltetO1-2

Available since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pJFRC156-21XUAS-B2RT>-dSTOP-B2RT>-myr::RFP

Plasmid

#32135

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

B2RT> STOP STOP B2RT>

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pXP831

Plasmid

#46065

Depositor

Nancy DaSilva

Article

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

CUP1

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pXP314

Plasmid

#26835

Depositor

Suzanne Sandmeyer, Nancy DaSilva

Article

Fang et al Yeast. 2010 Sep 10. ():.

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pGST1-Osh4 (2-434)

Plasmid

#17632

Depositor

James Hurley

Article

Im et al Nature. 2005 Sep 1. 437(7055):154-8.

Insert

Osh4 (KES1 Budding Yeast)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only
pFA6a-kanMX-tetO-Pcyc1-3xHA

Plasmid

#41024

Depositor

Michael Nick Boddy

Article

Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

Insert

tetO7-Pcyc1-3xHA

Use

N-terminal tagging/promoter replacement

Tags

Expression

Mutation

Promoter

Available since

Dec. 14, 2012

Availability

Academic Institutions and Nonprofits only

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pBlueScript NCX1.1

Plasmid

#66799

Depositor

Michela Ottolia, Kenneth Philipson

Article

Nicoll et al Science. 1990 Oct 26;250(4980):562-5.

Insert

NCX1.1

Use

T3 transcription of gene

Tags

Expression

Mutation

there are 3 silent mutations within the open read…

Promoter

T3

Available since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pSK15

Plasmid

#72855

Purpose

template for insertion of attP site (C-terminal tag plasmid design), Blasticidin-Resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

attP site

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3con-miR143Per

Plasmid

#21118

Depositor

Joshua Mendell

Article

Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 9. ()

Insert

hsa-miR-143 sensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pXP118

Plasmid

#26825

Depositor

Suzanne Sandmeyer, Nancy DaSilva

Article

Fang et al Yeast. 2010 Sep 10. ():.

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pKG204

Plasmid

#63078

Purpose

Retroviral expression of EGFP-TEV-S-tag HP1 alpha for protein localization and affinity purification (LAP)

Depositor

Iain Cheeseman

Insert

HP1 alpha (CBX5 Human)

Use

Retroviral

Tags

EGFP and TEV-S

Expression

Mammalian

Mutation

V151I mutation compared to GenBank reference sequ…

Promoter

Available since

Jan. 13, 2016

Availability

Academic Institutions and Nonprofits only

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SG-C-Int-5

Plasmid

#117034

Purpose

Constitutive integrase cassette - Bxb1-Tp901

Depositor

Jerome Bonnet

Article

Guiziou et al Nat Commun. 2019 Jan 28;10(1):456. doi:

Insert

Constitutive integrase cassette - Bxb1-Tp901

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pRB33

Plasmid

#72867

Purpose

template for N-terminal 3x FLAG tag, constitutive Puromycin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

3xFlag tag

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pVITRO1-SS-267

Plasmid

#68770

Purpose

Sensor pair 1 V1

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

C-terminal Sensor 1 V1
N-Terminal Sensor 1 V1

Use

Tags

NLS, flag, and nls

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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