Addgene

We narrowed to 25,972 results for: LEA

Showing: 10801 - 10820 of 25972 results

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pBB0405-TEV-His12

Plasmid

#137040

Purpose

expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0405 with C-terminal TEV-protease-cleavable His12

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Insert

AAC66795.1 (BB_0405 Borrelia burgdorferi B31)

Use

Tags

His12 (TEV protease cleavable)

Expression

Bacterial

Mutation

wild type, full-length protein, contains signal p…

Promoter

T7

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pRevA-TEV-His12

Plasmid

#137034

Purpose

expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi RevA with C-terminal TEV-protease-cleavable His12

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Insert

AAF07416.1 (revA Borrelia burgdorferi B31)

Use

Tags

His12 (TEV protease cleavable)

Expression

Bacterial

Mutation

wild type, full-length protein, contains signal p…

Promoter

T7

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pP13-TEV-His12

Plasmid

#137035

Purpose

expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi P13 with C-terminal TEV-protease-cleavable His12

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Insert

AAC66426.1 (BB_0034 Borrelia burgdorferi B31)

Use

Tags

His12 (TEV protease cleavable)

Expression

Bacterial

Mutation

wild type, full-length protein, contains signal p…

Promoter

T7

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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ID1GFP Donor

Plasmid

#119282

Purpose

Mouse ID1-eGFP for HR to create reporter

Depositor

Joan Massague

Article

Huang et al Cancer Discov. 2019 Oct 3. pii: 2159-829

Insert

Id1GFP + homology arms (Id1 Mouse)

Use

Reporter

Tags

eGFP

Expression

Mutation

Promoter

Available since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-NOS-IN133.3xFLAG-WPRE

Plasmid

#127869

Purpose

pAAV plasmid expressing an NOS-IN133.3xFLAG fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

Nos1 (Nos1 Mouse)

Use

AAV

Tags

3xFLAG

Expression

Mutation

Amino acids 1-133 only

Promoter

hSyn

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-KAT14-547-782

Plasmid

#118376

Purpose

Gateway compatible donor vector with KAT14-547-782 truncation.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

KAT14 (KAT14 Human)

Use

Donor-plasmid

Tags

Expression

Mutation

Truncation (aa 547-782)

Promoter

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-KAT14-1-546

Plasmid

#118377

Purpose

Gateway compatible donor vector with KAT14-1-546 truncation.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

KAT14 (KAT14 Human)

Use

Donor-plasmid

Tags

Expression

Mutation

Truncation (aa 1-546)

Promoter

Available since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pTKan-p35S::Csy4-pC4H::cogRFP (C90, JBEI-15908)

Plasmid

#110143

Purpose

Transformation and expression of Csy4 and cogDsRed proteins in plants

Depositor

Dominique Loque

Article

Liang et al ACS Synth Biol. 2017 May 19;6(5):806-816

Insert

Csy4 (ATCS Mustard Weed)

Use

Binary vector

Tags

cogDsRed

Expression

Bacterial and Plant

Mutation

Promoter

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTKan-p35S::Csy4-pC4H::cogGFP (C44, JBEI-16338)

Plasmid

#110144

Purpose

Transformation and expression of Csy4 and cogGFP proteins in plants

Depositor

Dominique Loque

Article

Liang et al ACS Synth Biol. 2017 May 19;6(5):806-816

Insert

Csy4 (ATCS Mustard Weed)

Use

Tags

cogGFP

Expression

Bacterial and Plant

Mutation

Promoter

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pQCXIX_sh_hMIB2_#9

Plasmid

#75255

Purpose

retroviral shRNA vector against human MIB2, expresses GFP for transduction control

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

shRNA against MIB2 (human) (MIB2 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

June 20, 2016

Availability

Academic Institutions and Nonprofits only

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mGDF8 donor CFP-hDF

Plasmid

#72590

Purpose

Plasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

Depositor

Renzhi Han

Article

Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

Insert

dysferlin (DYSF Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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hGDF8 donor CFP-hDF

Plasmid

#72591

Purpose

Plasmid contains left and right homology arms for human GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

Depositor

Renzhi Han

Article

Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

Insert

dysferlin (DYSF Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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Hoxb6-3'UTR

Plasmid

#31523

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

Hoxb6-3'UTR (Hoxb6 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only
pLVX-XBP1 mNeonGreen NLS

Plasmid

#115968

Purpose

ER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreen

Depositor

David Andrews

Article

Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

Insert

X-box binding protein 1 (XBP1 Human, Synthetic)

Use

Lentiviral

Tags

HA tag, c-myc NLS, and mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Grb10-WT

Plasmid

#74501

Purpose

Overexpression of Grb10 in mammalian cells

Depositor

Lars Rönnstrand

Article

Kazi et al Mol Oncol. 2013 Jun;7(3):402-18. doi: 10

Insert

Grb10 (GRB10 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

L358S, V522A, and V524L

Promoter

CMV

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
Lentiviral-S-tdTomato

Plasmid

#112579

Purpose

Expressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vector

Depositor

Jeffrey Rothstein

Article

Zhang et al Nature. 2015 Sep 3;525(7567):56-61. doi:

Insert

NLS-tdTomato-NES

Use

Lentiviral

Tags

nuclear export sequence and nuclear localization …

Expression

Mammalian

Mutation

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin

Plasmid

#134868

Purpose

Expresses EGFP-tagged lysosomal marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Lysosomal-associated membrane protein 1 (LAMP1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD81-(TEV)-FKBP-SmBiT

Plasmid

#182651

Purpose

human CD81-TEV cleavage site-FKBP-SmBiT tag

Depositor

Masaharu Somiya

Article

Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

Insert

human CD81-TEVp cleavage site-FKBP-SmBiT (CD81 Human, Synthetic)

Use

Tags

SmBiT

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pZT-C13-R1

Plasmid

#62197

Purpose

Chr.13/CLYBL TALEN expression vector

Depositor

Jizhong Zou

Article

Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

Insert

Right CLYBL TALEN

Use

TALEN

Tags

Expression

Mutation

Promoter

CMV

Available since

April 22, 2015

Availability

Academic Institutions and Nonprofits only

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pZT-C13-L1

Plasmid

#62196

Purpose

Chr.13/CLYBL TALEN expression vector

Depositor

Jizhong Zou

Article

Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

Insert

Left CLYBL TALEN

Use

TALEN

Tags

Expression

Mutation

Promoter

CMV

Available since

April 22, 2015

Availability

Academic Institutions and Nonprofits only

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