Showing: 10801 - 10820 of 14149 results
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Plasmid#42337PurposeDual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.DepositorArticleInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET30-GBF-E6N
Plasmid#21699DepositorInsertProtein G domain B1 fused N-terminal zinc binding domain of human papillomavirus type 16 (residues 2-74) (E6 Human papillomavirus type 16)
UseTagsProtein G B1 domainExpressionBacterialMutationGB1 (1-56aa) fusion tag with mutations at three p…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-Flag-hDcp2
Plasmid#72213Purposemammalian expression of Flag-tagged human Dcp2DepositorInsertDcp2 (DCP2 Human)
UseTagsFlagExpressionMammalianMutationQ170R (please see depositor's comment below)PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-hDcp2
Plasmid#72216Purposebacterial expression of GST-tagged human Dcp2DepositorInsertDcp2 (DCP2 Human)
UseTagsGSTExpressionBacterialMutationF146Y and E198K (please see depositor's comm…PromotertacAvailabilityAcademic Institutions and Nonprofits only -
MSP2262
Plasmid#70703PurposeBacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1DepositorInsertsmammalian codon-optimized Staphylococcus aureus dCas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationD10A & H557A in SaCas9PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
MSP2279
Plasmid#70706PurposeBacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2DepositorInsertsmammalian codon-optimized Staphylococcus aureus dCas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationD10A and H557A in SaCas9PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pLEGFP-S727A-STAT3
Plasmid#71446PurposeRetroviral expression of S727A-STAT3. Please note that this plasmid contains S727A-STAT3 tagged with FLAG, not GFP.DepositorInsertSTAT3 (STAT3 Human)
UseRetroviralTagsFLAGExpressionMutationS727APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLEGFP-K49R-STAT3
Plasmid#71447PurposeRetroviral expression of K49R-STAT3. Please note that this plasmid contains K49R-STAT3 tagged with FLAG, not GFP.DepositorInsertSTAT3 (STAT3 Human)
UseRetroviralTagsFLAGExpressionMutationK49RPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLEGFP-K140R-STAT3
Plasmid#71448PurposeRetroviral expression of K140R-STAT3. Please note that this plasmid contains K140R-STAT3 tagged with FLAG, not GFP.DepositorInsertSTAT3 (STAT3 Human)
UseRetroviralTagsFLAGExpressionMutationK140RPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLEGFP-K685R-STAT3
Plasmid#71449PurposeRetroviral expression of K685R-STAT3. Please note that this plasmid contains K685R-STAT3 tagged with FLAG, not GFP.DepositorInsertSTAT3 (STAT3 Human)
UseRetroviralTagsFLAGExpressionMutationK685RPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLEGFP-WT-STAT3
Plasmid#71450PurposeRetroviral expression of WT-STAT3. Please note that this plasmid contains WT-STAT3 tagged with FLAG, not GFP.DepositorInsertSTAT3 (STAT3 Human)
UseRetroviralTagsFLAGExpressionMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pNUT N6His mICA NG
Plasmid#70162PurposeExpresses N-His tagged nonglycosylated mouse inhibitor of carbonic anhydraseDepositorInsertmouse inhibitor of carbonic anhydrase
UseTagsN-terminal signal peptide, 4 aa link, 6 His, Fact…ExpressionMammalianMutationAsn470Asp and Asn645AspPromoterSV40AvailabilityAcademic Institutions and Nonprofits only -
pCMV-calpainsensor
Plasmid#36182Purposedetection of activity of ubiquitous calpainsDepositorInsertalpha-Fodrin cleavage site (Sptan1 Mouse)
UseTagseCFP and eYFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
GRLF1
Plasmid#36195PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertGRLF1 (Codon Devices Synthesized) (ARHGAP35 Human)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationContains the Ras homolog domain only (amino acid …PromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pShuttle-FEN1DA
Plasmid#35028DepositorInsertFEN1DA (FEN1 Human)
UseAdenoviralTagsFlagExpressionMammalianMutationD181A--NUCLEASE DEAD POINT MUTANTPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
L3MBTL3 (3UT1)
Plasmid#36901PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertL3MBTL3 (L3MBTL3 Human)
UseTagsHis tag and Streptavidin-Binding Peptide (SBP)-Ta…ExpressionBacterialMutationcontains amino acid residues 228-551 onlyPromoterAvailabilityIndustry, Academic Institutions, and Nonprofits -
pEG593
Plasmid#40119DepositorInsertMex-5 (ATG through 648 bp of 3'UTR) R274E K318E (RNAi res) (mex-5 Nematode)
UseGateway destination vectorTagsDendra2 PAFP (sequence optimized for worm express…ExpressionWormMutationR274E, K318E, Exon 2 recoded to be RNAi-resistantPromoter4.4 kb mex-5 promoter fragmentAvailabilityAcademic Institutions and Nonprofits only -
pEG628
Plasmid#40120DepositorInsertMex-5 (aa1-244) (RNAi resistant) (mex-5 Nematode)
UseGateway destination vectorTagsDendra2 PAFP (sequence optimized for worm express…ExpressionWormMutationcontains aa1-244, Exon 2 recoded to be RNAi-resis…Promoter4.4 kb mex-5 promoter fragmentAvailabilityAcademic Institutions and Nonprofits only -
pEG610
Plasmid#40195DepositorInsertMex-5 (aa1-355) (RNAi resistant) (mex-5 Nematode)
UseTagsDendra2 PAFP (sequence optimized for worm express…ExpressionWormMutationcontains aa 1-355; exon 2 recoded to be RNAi-resi…Promoter4.4 kb mex-5 promoter fragmentAvailabilityAcademic Institutions and Nonprofits only -
pTH734-CEN-minHIS3
Plasmid#38227DepositorInsertHIS3 (HIS3 Budding Yeast, Synthetic)
UseTagsHAExpressionYeastMutationEvery codon of the coding sequence has been repla…PromoterTDH3AvailabilityAcademic Institutions and Nonprofits only
Showing: 10801 - 10820 of 14149 results